Cargando…
Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids
An organoid is a three-dimensional multicellular structure that shows realistic micro-anatomy of an organ. This in vitro model mimics the in vivo environment, architecture and multi-lineage differentiation of the original organs and allows to answer many interesting biological questions. For these r...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Journal of Biological Methods
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865078/ https://www.ncbi.nlm.nih.gov/pubmed/33564693 http://dx.doi.org/10.14440/jbm.2020.334 |
_version_ | 1783647771606122496 |
---|---|
author | Lallemant, Louison Lebreton, Corinne Garfa-Traoré, Meriem |
author_facet | Lallemant, Louison Lebreton, Corinne Garfa-Traoré, Meriem |
author_sort | Lallemant, Louison |
collection | PubMed |
description | An organoid is a three-dimensional multicellular structure that shows realistic micro-anatomy of an organ. This in vitro model mimics the in vivo environment, architecture and multi-lineage differentiation of the original organs and allows to answer many interesting biological questions. For these reasons, they are widely used in stem cell, regenerative medicine, toxicology, pharmacology, and host-microbe interactions research. In order to study organoids, microscopy is very useful: It is possible to make three-dimensional reconstruction of serial sections but it is time consuming and error-prone. Here we propose an alternative solution: Tissue clearing reduces the dispersion of light because it homogenizes the refractive index of the tissue, allowing sample observation throughout its thickness. We have compared different clearing techniques on mouse intestinal organoids using different acquisition methods. |
format | Online Article Text |
id | pubmed-7865078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Journal of Biological Methods |
record_format | MEDLINE/PubMed |
spelling | pubmed-78650782021-02-08 Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids Lallemant, Louison Lebreton, Corinne Garfa-Traoré, Meriem J Biol Methods Benchmark An organoid is a three-dimensional multicellular structure that shows realistic micro-anatomy of an organ. This in vitro model mimics the in vivo environment, architecture and multi-lineage differentiation of the original organs and allows to answer many interesting biological questions. For these reasons, they are widely used in stem cell, regenerative medicine, toxicology, pharmacology, and host-microbe interactions research. In order to study organoids, microscopy is very useful: It is possible to make three-dimensional reconstruction of serial sections but it is time consuming and error-prone. Here we propose an alternative solution: Tissue clearing reduces the dispersion of light because it homogenizes the refractive index of the tissue, allowing sample observation throughout its thickness. We have compared different clearing techniques on mouse intestinal organoids using different acquisition methods. Journal of Biological Methods 2020-12-28 /pmc/articles/PMC7865078/ /pubmed/33564693 http://dx.doi.org/10.14440/jbm.2020.334 Text en © 2013-2020 The Journal of Biological Methods, All rights reserved. http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License: http://creativecommons.org/licenses/by-nc-sa/4.0 |
spellingShingle | Benchmark Lallemant, Louison Lebreton, Corinne Garfa-Traoré, Meriem Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids |
title | Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids |
title_full | Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids |
title_fullStr | Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids |
title_full_unstemmed | Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids |
title_short | Comparison of different clearing and acquisition methods for 3D imaging of murine intestinal organoids |
title_sort | comparison of different clearing and acquisition methods for 3d imaging of murine intestinal organoids |
topic | Benchmark |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865078/ https://www.ncbi.nlm.nih.gov/pubmed/33564693 http://dx.doi.org/10.14440/jbm.2020.334 |
work_keys_str_mv | AT lallemantlouison comparisonofdifferentclearingandacquisitionmethodsfor3dimagingofmurineintestinalorganoids AT lebretoncorinne comparisonofdifferentclearingandacquisitionmethodsfor3dimagingofmurineintestinalorganoids AT garfatraoremeriem comparisonofdifferentclearingandacquisitionmethodsfor3dimagingofmurineintestinalorganoids |