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Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity

Pharmacodynamic interactions between plant isolated compounds are important to understand the mode of action of an herbal extract to formulate or create better standardized extracts, phytomedicines, or phytopharmaceuticals. In this work, we propose binary mixtures using a leader compound to found ph...

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Autores principales: Arango-De la Pava, Luis David, Zamilpa, Alejandro, Trejo-Espino, José Luis, Domínguez-Mendoza, Blanca Eda, Jiménez-Ferrer, Enrique, Pérez-Martínez, Leonor, Trejo-Tapia, Gabriela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865359/
https://www.ncbi.nlm.nih.gov/pubmed/33494340
http://dx.doi.org/10.3390/molecules26030547
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author Arango-De la Pava, Luis David
Zamilpa, Alejandro
Trejo-Espino, José Luis
Domínguez-Mendoza, Blanca Eda
Jiménez-Ferrer, Enrique
Pérez-Martínez, Leonor
Trejo-Tapia, Gabriela
author_facet Arango-De la Pava, Luis David
Zamilpa, Alejandro
Trejo-Espino, José Luis
Domínguez-Mendoza, Blanca Eda
Jiménez-Ferrer, Enrique
Pérez-Martínez, Leonor
Trejo-Tapia, Gabriela
author_sort Arango-De la Pava, Luis David
collection PubMed
description Pharmacodynamic interactions between plant isolated compounds are important to understand the mode of action of an herbal extract to formulate or create better standardized extracts, phytomedicines, or phytopharmaceuticals. In this work, we propose binary mixtures using a leader compound to found pharmacodynamic interactions in inhibition of the NF-κB/AP-1 pathway using RAW-Blue™ cells. Eight compounds were isolated from Castilleja tenuiflora, four were new furofuran-type lignans for the species magnolin, eudesmin, sesamin, and kobusin. Magnolin (60.97%) was the most effective lignan inhibiting the NF-κB/AP-1 pathway, followed by eudesmin (56.82%), tenuifloroside (52.91%), sesamin (52.63%), and kobusin (45.45%). Verbascoside, a major compound contained in wild C. tenuiflora showed an inhibitory effect on NF-κB/AP-1. This polyphenol was chosen as a leader compound for binary mixtures. Verbacoside-aucubin and verbascoside-kobusin produced synergism, while verbascoside-tenuifloroside had subadditivity in all concentrations. Verbascoside-kobusin is a promising mixture to use on NF-κB/AP-1 related diseases and anti-inflammatory C. tenuiflora-based phytomedicines.
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spelling pubmed-78653592021-02-07 Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity Arango-De la Pava, Luis David Zamilpa, Alejandro Trejo-Espino, José Luis Domínguez-Mendoza, Blanca Eda Jiménez-Ferrer, Enrique Pérez-Martínez, Leonor Trejo-Tapia, Gabriela Molecules Article Pharmacodynamic interactions between plant isolated compounds are important to understand the mode of action of an herbal extract to formulate or create better standardized extracts, phytomedicines, or phytopharmaceuticals. In this work, we propose binary mixtures using a leader compound to found pharmacodynamic interactions in inhibition of the NF-κB/AP-1 pathway using RAW-Blue™ cells. Eight compounds were isolated from Castilleja tenuiflora, four were new furofuran-type lignans for the species magnolin, eudesmin, sesamin, and kobusin. Magnolin (60.97%) was the most effective lignan inhibiting the NF-κB/AP-1 pathway, followed by eudesmin (56.82%), tenuifloroside (52.91%), sesamin (52.63%), and kobusin (45.45%). Verbascoside, a major compound contained in wild C. tenuiflora showed an inhibitory effect on NF-κB/AP-1. This polyphenol was chosen as a leader compound for binary mixtures. Verbacoside-aucubin and verbascoside-kobusin produced synergism, while verbascoside-tenuifloroside had subadditivity in all concentrations. Verbascoside-kobusin is a promising mixture to use on NF-κB/AP-1 related diseases and anti-inflammatory C. tenuiflora-based phytomedicines. MDPI 2021-01-21 /pmc/articles/PMC7865359/ /pubmed/33494340 http://dx.doi.org/10.3390/molecules26030547 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arango-De la Pava, Luis David
Zamilpa, Alejandro
Trejo-Espino, José Luis
Domínguez-Mendoza, Blanca Eda
Jiménez-Ferrer, Enrique
Pérez-Martínez, Leonor
Trejo-Tapia, Gabriela
Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity
title Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity
title_full Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity
title_fullStr Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity
title_full_unstemmed Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity
title_short Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity
title_sort synergism and subadditivity of verbascoside-lignans and -iridoids binary mixtures isolated from castilleja tenuiflora benth. on nf-κb/ap-1 inhibition activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865359/
https://www.ncbi.nlm.nih.gov/pubmed/33494340
http://dx.doi.org/10.3390/molecules26030547
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