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Genetically Encoded Biosensors Based on Fluorescent Proteins
Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, differ...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865379/ https://www.ncbi.nlm.nih.gov/pubmed/33504068 http://dx.doi.org/10.3390/s21030795 |
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author | Kim, Hyunbin Ju, Jeongmin Lee, Hae Nim Chun, Hyeyeon Seong, Jihye |
author_facet | Kim, Hyunbin Ju, Jeongmin Lee, Hae Nim Chun, Hyeyeon Seong, Jihye |
author_sort | Kim, Hyunbin |
collection | PubMed |
description | Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs. |
format | Online Article Text |
id | pubmed-7865379 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78653792021-02-07 Genetically Encoded Biosensors Based on Fluorescent Proteins Kim, Hyunbin Ju, Jeongmin Lee, Hae Nim Chun, Hyeyeon Seong, Jihye Sensors (Basel) Review Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs. MDPI 2021-01-25 /pmc/articles/PMC7865379/ /pubmed/33504068 http://dx.doi.org/10.3390/s21030795 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Kim, Hyunbin Ju, Jeongmin Lee, Hae Nim Chun, Hyeyeon Seong, Jihye Genetically Encoded Biosensors Based on Fluorescent Proteins |
title | Genetically Encoded Biosensors Based on Fluorescent Proteins |
title_full | Genetically Encoded Biosensors Based on Fluorescent Proteins |
title_fullStr | Genetically Encoded Biosensors Based on Fluorescent Proteins |
title_full_unstemmed | Genetically Encoded Biosensors Based on Fluorescent Proteins |
title_short | Genetically Encoded Biosensors Based on Fluorescent Proteins |
title_sort | genetically encoded biosensors based on fluorescent proteins |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865379/ https://www.ncbi.nlm.nih.gov/pubmed/33504068 http://dx.doi.org/10.3390/s21030795 |
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