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Genetically Encoded Biosensors Based on Fluorescent Proteins

Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, differ...

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Detalles Bibliográficos
Autores principales: Kim, Hyunbin, Ju, Jeongmin, Lee, Hae Nim, Chun, Hyeyeon, Seong, Jihye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865379/
https://www.ncbi.nlm.nih.gov/pubmed/33504068
http://dx.doi.org/10.3390/s21030795
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author Kim, Hyunbin
Ju, Jeongmin
Lee, Hae Nim
Chun, Hyeyeon
Seong, Jihye
author_facet Kim, Hyunbin
Ju, Jeongmin
Lee, Hae Nim
Chun, Hyeyeon
Seong, Jihye
author_sort Kim, Hyunbin
collection PubMed
description Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs.
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spelling pubmed-78653792021-02-07 Genetically Encoded Biosensors Based on Fluorescent Proteins Kim, Hyunbin Ju, Jeongmin Lee, Hae Nim Chun, Hyeyeon Seong, Jihye Sensors (Basel) Review Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time monitoring of molecular dynamics in space and time, which are crucial for the proper functioning and regulation of complex cellular processes. Depending on the types of molecular events to be monitored, different sensing strategies need to be applied for the best design of FP-based biosensors. Here, we review genetically encoded biosensors based on FPs with various sensing strategies, for example, translocation, fluorescence resonance energy transfer (FRET), reconstitution of split FP, pH sensitivity, maturation speed, and so on. We introduce general principles of each sensing strategy and discuss critical factors to be considered if available, then provide representative examples of these FP-based biosensors. These will help in designing the best sensing strategy for the successful development of new genetically encoded biosensors based on FPs. MDPI 2021-01-25 /pmc/articles/PMC7865379/ /pubmed/33504068 http://dx.doi.org/10.3390/s21030795 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kim, Hyunbin
Ju, Jeongmin
Lee, Hae Nim
Chun, Hyeyeon
Seong, Jihye
Genetically Encoded Biosensors Based on Fluorescent Proteins
title Genetically Encoded Biosensors Based on Fluorescent Proteins
title_full Genetically Encoded Biosensors Based on Fluorescent Proteins
title_fullStr Genetically Encoded Biosensors Based on Fluorescent Proteins
title_full_unstemmed Genetically Encoded Biosensors Based on Fluorescent Proteins
title_short Genetically Encoded Biosensors Based on Fluorescent Proteins
title_sort genetically encoded biosensors based on fluorescent proteins
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865379/
https://www.ncbi.nlm.nih.gov/pubmed/33504068
http://dx.doi.org/10.3390/s21030795
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