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Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection

Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological compl...

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Autores principales: Vlcnovska, Marcela, Stossova, Aneta, Kuchynka, Michaela, Dillingerova, Veronika, Polanska, Hana, Masarik, Michal, Hrstka, Roman, Adam, Vojtech, Kanicky, Viktor, Vaculovic, Tomas, Vaculovicova, Marketa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865668/
https://www.ncbi.nlm.nih.gov/pubmed/33530345
http://dx.doi.org/10.3390/molecules26030630
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author Vlcnovska, Marcela
Stossova, Aneta
Kuchynka, Michaela
Dillingerova, Veronika
Polanska, Hana
Masarik, Michal
Hrstka, Roman
Adam, Vojtech
Kanicky, Viktor
Vaculovic, Tomas
Vaculovicova, Marketa
author_facet Vlcnovska, Marcela
Stossova, Aneta
Kuchynka, Michaela
Dillingerova, Veronika
Polanska, Hana
Masarik, Michal
Hrstka, Roman
Adam, Vojtech
Kanicky, Viktor
Vaculovic, Tomas
Vaculovicova, Marketa
author_sort Vlcnovska, Marcela
collection PubMed
description Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1–14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.
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spelling pubmed-78656682021-02-07 Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection Vlcnovska, Marcela Stossova, Aneta Kuchynka, Michaela Dillingerova, Veronika Polanska, Hana Masarik, Michal Hrstka, Roman Adam, Vojtech Kanicky, Viktor Vaculovic, Tomas Vaculovicova, Marketa Molecules Article Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1–14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein. MDPI 2021-01-26 /pmc/articles/PMC7865668/ /pubmed/33530345 http://dx.doi.org/10.3390/molecules26030630 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vlcnovska, Marcela
Stossova, Aneta
Kuchynka, Michaela
Dillingerova, Veronika
Polanska, Hana
Masarik, Michal
Hrstka, Roman
Adam, Vojtech
Kanicky, Viktor
Vaculovic, Tomas
Vaculovicova, Marketa
Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
title Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
title_full Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
title_fullStr Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
title_full_unstemmed Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
title_short Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
title_sort comparison of metal nanoparticles (au, ag, eu, cd) used for immunoanalysis using la-icp-ms detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865668/
https://www.ncbi.nlm.nih.gov/pubmed/33530345
http://dx.doi.org/10.3390/molecules26030630
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