Cargando…

The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells

Pigment epithelium derived factor (PEDF) is a multifunctional secretory soluble glycoprotein that belongs to the serine protease inhibitor (serpin) family. It was reported to have neurotrophic, anti-angiogenic and anti-tumorigenic activity. Recently, PEDF was found in testicular peritubular cells an...

Descripción completa

Detalles Bibliográficos
Autores principales: Bagdadi, Noy, Sawaied, Alaa, AbuMadighem, Ali, Lunenfeld, Eitan, Huleihel, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865766/
https://www.ncbi.nlm.nih.gov/pubmed/33498962
http://dx.doi.org/10.3390/ijms22031147
_version_ 1783647923974701056
author Bagdadi, Noy
Sawaied, Alaa
AbuMadighem, Ali
Lunenfeld, Eitan
Huleihel, Mahmoud
author_facet Bagdadi, Noy
Sawaied, Alaa
AbuMadighem, Ali
Lunenfeld, Eitan
Huleihel, Mahmoud
author_sort Bagdadi, Noy
collection PubMed
description Pigment epithelium derived factor (PEDF) is a multifunctional secretory soluble glycoprotein that belongs to the serine protease inhibitor (serpin) family. It was reported to have neurotrophic, anti-angiogenic and anti-tumorigenic activity. Recently, PEDF was found in testicular peritubular cells and it was assumed to be involved in the avascular nature of seminiferous tubules. The aim of this study was to determine the cellular origin, expression levels and target cells of PEDF in testicular tissue of immature and adult mice under physiological conditions, and to explore its possible role in the process of spermatogenesis in vitro. Using immunofluorescence staining, we showed that PEDF was localized in spermatogenic cells at different stages of development as well as in the somatic cells of the testis. Its protein levels in testicular homogenates and Sertoli cells supernatant showed a significant decrease with age. PEDF receptor (PEDF-R) was localized within the seminiferous tubule cells and in the interstitial cells compartment. Its RNA expression levels showed an increase with age until 8 weeks followed by a decrease. RNA levels of PEDF-R showed the opposite trend of the protein. Addition of PEDF to cultures of isolated cells from the seminiferous tubules did not changed their proliferation rate, however, a significant increase was observed in number of meiotic/post meiotic cells at 1000 ng/mL of PEDF; indicating an in vitro differentiation effect. This study may suggest a role for PEDF in the process of spermatogenesis.
format Online
Article
Text
id pubmed-7865766
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78657662021-02-07 The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells Bagdadi, Noy Sawaied, Alaa AbuMadighem, Ali Lunenfeld, Eitan Huleihel, Mahmoud Int J Mol Sci Article Pigment epithelium derived factor (PEDF) is a multifunctional secretory soluble glycoprotein that belongs to the serine protease inhibitor (serpin) family. It was reported to have neurotrophic, anti-angiogenic and anti-tumorigenic activity. Recently, PEDF was found in testicular peritubular cells and it was assumed to be involved in the avascular nature of seminiferous tubules. The aim of this study was to determine the cellular origin, expression levels and target cells of PEDF in testicular tissue of immature and adult mice under physiological conditions, and to explore its possible role in the process of spermatogenesis in vitro. Using immunofluorescence staining, we showed that PEDF was localized in spermatogenic cells at different stages of development as well as in the somatic cells of the testis. Its protein levels in testicular homogenates and Sertoli cells supernatant showed a significant decrease with age. PEDF receptor (PEDF-R) was localized within the seminiferous tubule cells and in the interstitial cells compartment. Its RNA expression levels showed an increase with age until 8 weeks followed by a decrease. RNA levels of PEDF-R showed the opposite trend of the protein. Addition of PEDF to cultures of isolated cells from the seminiferous tubules did not changed their proliferation rate, however, a significant increase was observed in number of meiotic/post meiotic cells at 1000 ng/mL of PEDF; indicating an in vitro differentiation effect. This study may suggest a role for PEDF in the process of spermatogenesis. MDPI 2021-01-24 /pmc/articles/PMC7865766/ /pubmed/33498962 http://dx.doi.org/10.3390/ijms22031147 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bagdadi, Noy
Sawaied, Alaa
AbuMadighem, Ali
Lunenfeld, Eitan
Huleihel, Mahmoud
The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells
title The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells
title_full The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells
title_fullStr The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells
title_full_unstemmed The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells
title_short The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells
title_sort expression levels and cellular localization of pigment epithelium derived factor (pedf) in mouse testis: its possible involvement in the differentiation of spermatogonial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865766/
https://www.ncbi.nlm.nih.gov/pubmed/33498962
http://dx.doi.org/10.3390/ijms22031147
work_keys_str_mv AT bagdadinoy theexpressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT sawaiedalaa theexpressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT abumadighemali theexpressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT lunenfeldeitan theexpressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT huleihelmahmoud theexpressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT bagdadinoy expressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT sawaiedalaa expressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT abumadighemali expressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT lunenfeldeitan expressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells
AT huleihelmahmoud expressionlevelsandcellularlocalizationofpigmentepitheliumderivedfactorpedfinmousetestisitspossibleinvolvementinthedifferentiationofspermatogonialcells