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Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice

Numerous evidence corroborates roles of gap junctions/hemichannels in proper kidney development. We analyzed how Dab1 gene functional silencing influences expression and localization of Cx37, Cx40, Cx43, Cx45, Panx1 and renin in postnatal kidneys of yotari mice, by using immunohistochemistry and ele...

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Autores principales: Lozić, Mirela, Filipović, Natalija, Jurić, Marija, Kosović, Ivona, Benzon, Benjamin, Šolić, Ivana, Kelam, Nela, Racetin, Anita, Watanabe, Koichiro, Katsuyama, Yu, Ogata, Masaki, Saraga-Babić, Mirna, Vukojević, Katarina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865779/
https://www.ncbi.nlm.nih.gov/pubmed/33525532
http://dx.doi.org/10.3390/ijms22031284
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author Lozić, Mirela
Filipović, Natalija
Jurić, Marija
Kosović, Ivona
Benzon, Benjamin
Šolić, Ivana
Kelam, Nela
Racetin, Anita
Watanabe, Koichiro
Katsuyama, Yu
Ogata, Masaki
Saraga-Babić, Mirna
Vukojević, Katarina
author_facet Lozić, Mirela
Filipović, Natalija
Jurić, Marija
Kosović, Ivona
Benzon, Benjamin
Šolić, Ivana
Kelam, Nela
Racetin, Anita
Watanabe, Koichiro
Katsuyama, Yu
Ogata, Masaki
Saraga-Babić, Mirna
Vukojević, Katarina
author_sort Lozić, Mirela
collection PubMed
description Numerous evidence corroborates roles of gap junctions/hemichannels in proper kidney development. We analyzed how Dab1 gene functional silencing influences expression and localization of Cx37, Cx40, Cx43, Cx45, Panx1 and renin in postnatal kidneys of yotari mice, by using immunohistochemistry and electron microscopy. Dab1 Δ102/221 might lead to the activation of c-Src tyrosine kinase, causing the upregulation of Cx43 in the medulla of yotari mice. The expression of renin was more prominent in yotari mice (p < 0.001). Renin granules were unusually present inside the vascular walls of glomeruli capillaries, in proximal and distal convoluted tubules and in the medulla. Disfunction of Cx40 is likely responsible for increased atypically positioned renin cells which release renin in an uncontrolled fashion, but this doesn’t rule out simultaneous involvement of other Cxs, such as Cx45 which was significantly increased in the yotari cortex. The decreased Cx37 expression in yotari medulla might contribute to hypertension reduction provoked by high renin expression. These findings imply the relevance of Cxs/Panx1 as markers of impaired kidney function (high renin) in yotari mice and that they have a role in the preservation of intercellular signaling and implicate connexopathies as the cause of premature death of yotari mice.
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spelling pubmed-78657792021-02-07 Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice Lozić, Mirela Filipović, Natalija Jurić, Marija Kosović, Ivona Benzon, Benjamin Šolić, Ivana Kelam, Nela Racetin, Anita Watanabe, Koichiro Katsuyama, Yu Ogata, Masaki Saraga-Babić, Mirna Vukojević, Katarina Int J Mol Sci Article Numerous evidence corroborates roles of gap junctions/hemichannels in proper kidney development. We analyzed how Dab1 gene functional silencing influences expression and localization of Cx37, Cx40, Cx43, Cx45, Panx1 and renin in postnatal kidneys of yotari mice, by using immunohistochemistry and electron microscopy. Dab1 Δ102/221 might lead to the activation of c-Src tyrosine kinase, causing the upregulation of Cx43 in the medulla of yotari mice. The expression of renin was more prominent in yotari mice (p < 0.001). Renin granules were unusually present inside the vascular walls of glomeruli capillaries, in proximal and distal convoluted tubules and in the medulla. Disfunction of Cx40 is likely responsible for increased atypically positioned renin cells which release renin in an uncontrolled fashion, but this doesn’t rule out simultaneous involvement of other Cxs, such as Cx45 which was significantly increased in the yotari cortex. The decreased Cx37 expression in yotari medulla might contribute to hypertension reduction provoked by high renin expression. These findings imply the relevance of Cxs/Panx1 as markers of impaired kidney function (high renin) in yotari mice and that they have a role in the preservation of intercellular signaling and implicate connexopathies as the cause of premature death of yotari mice. MDPI 2021-01-28 /pmc/articles/PMC7865779/ /pubmed/33525532 http://dx.doi.org/10.3390/ijms22031284 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lozić, Mirela
Filipović, Natalija
Jurić, Marija
Kosović, Ivona
Benzon, Benjamin
Šolić, Ivana
Kelam, Nela
Racetin, Anita
Watanabe, Koichiro
Katsuyama, Yu
Ogata, Masaki
Saraga-Babić, Mirna
Vukojević, Katarina
Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice
title Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice
title_full Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice
title_fullStr Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice
title_full_unstemmed Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice
title_short Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice
title_sort alteration of cx37, cx40, cx43, cx45, panx1, and renin expression patterns in postnatal kidneys of dab1-/- (yotari) mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865779/
https://www.ncbi.nlm.nih.gov/pubmed/33525532
http://dx.doi.org/10.3390/ijms22031284
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