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Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels
The temperature- and pH-responsive poly(N-isopropylacrylamide-co-acrylic acid), p(NIPAM-co-AA), copolymer was synthesized by free radical polymerization and examined as a carrier for modified release of biochanin A. Biochanin A is a biologically active methoxylated isoflavone which exhibits estrogen...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865815/ https://www.ncbi.nlm.nih.gov/pubmed/33572749 http://dx.doi.org/10.3390/polym13030426 |
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author | Gajić, Ivana Ilić-Stojanović, Snežana Dinić, Ana Zdravković, Aleksandar Stanojević, Ljiljana Nikolić, Vesna Nikolić, Ljubiša |
author_facet | Gajić, Ivana Ilić-Stojanović, Snežana Dinić, Ana Zdravković, Aleksandar Stanojević, Ljiljana Nikolić, Vesna Nikolić, Ljubiša |
author_sort | Gajić, Ivana |
collection | PubMed |
description | The temperature- and pH-responsive poly(N-isopropylacrylamide-co-acrylic acid), p(NIPAM-co-AA), copolymer was synthesized by free radical polymerization and examined as a carrier for modified release of biochanin A. Biochanin A is a biologically active methoxylated isoflavone which exhibits estrogenic and other pharmacological activities. Due to its poor aqueous solubility and extensive first-pass metabolism, biochanin A has low bioavailability. The aim of this work was to incorporate biochanin A into the synthesized p(NIPAM-co-AA) copolymer and to examine its release at the body temperature and pH values that correspond to pH values of vaginal and rectal cavities. The amount of released biochanin A was monitored by the ultra-visible spectroscopy (UV-Vis) method. The structure of synthesized p(NIPAM-co-AA) copolymer and copolymer with incorporated biochanin A were characterized by using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. The content of residual monomers in the synthesized copolymer was analyzed by using the high-pressure liquid chromatography (HPLC) method. The swelling behavior of p(NIPAM-co-AA) copolymer was monitored in relation to the temperature and pH values of the surrounding medium. For modelling the process of p(NIPAM-co-AA) copolymer swelling, the full three-level factorial design was applied. |
format | Online Article Text |
id | pubmed-7865815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78658152021-02-07 Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels Gajić, Ivana Ilić-Stojanović, Snežana Dinić, Ana Zdravković, Aleksandar Stanojević, Ljiljana Nikolić, Vesna Nikolić, Ljubiša Polymers (Basel) Article The temperature- and pH-responsive poly(N-isopropylacrylamide-co-acrylic acid), p(NIPAM-co-AA), copolymer was synthesized by free radical polymerization and examined as a carrier for modified release of biochanin A. Biochanin A is a biologically active methoxylated isoflavone which exhibits estrogenic and other pharmacological activities. Due to its poor aqueous solubility and extensive first-pass metabolism, biochanin A has low bioavailability. The aim of this work was to incorporate biochanin A into the synthesized p(NIPAM-co-AA) copolymer and to examine its release at the body temperature and pH values that correspond to pH values of vaginal and rectal cavities. The amount of released biochanin A was monitored by the ultra-visible spectroscopy (UV-Vis) method. The structure of synthesized p(NIPAM-co-AA) copolymer and copolymer with incorporated biochanin A were characterized by using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. The content of residual monomers in the synthesized copolymer was analyzed by using the high-pressure liquid chromatography (HPLC) method. The swelling behavior of p(NIPAM-co-AA) copolymer was monitored in relation to the temperature and pH values of the surrounding medium. For modelling the process of p(NIPAM-co-AA) copolymer swelling, the full three-level factorial design was applied. MDPI 2021-01-29 /pmc/articles/PMC7865815/ /pubmed/33572749 http://dx.doi.org/10.3390/polym13030426 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gajić, Ivana Ilić-Stojanović, Snežana Dinić, Ana Zdravković, Aleksandar Stanojević, Ljiljana Nikolić, Vesna Nikolić, Ljubiša Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
title | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
title_full | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
title_fullStr | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
title_full_unstemmed | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
title_short | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
title_sort | modified biochanin a release from dual ph- and thermo-responsive copolymer hydrogels |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865815/ https://www.ncbi.nlm.nih.gov/pubmed/33572749 http://dx.doi.org/10.3390/polym13030426 |
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