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Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization

This work is devoted to the development and optimization of the parameters of graphene-based sensors. The graphene films used in the present study were grown on semi-insulating 6H-SiC substrates by thermal decomposition of SiC at the temperature of ~1700 °C. The results of measurements by Auger and...

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Autores principales: Lebedev, Alexander A., Davydov, Sergey Yu, Eliseyev, Ilya A., Roenkov, Alexander D., Avdeev, Oleg, Lebedev, Sergey P., Makarov, Yurii, Puzyk, Mikhail, Klotchenko, Sergey, Usikov, Alexander S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865904/
https://www.ncbi.nlm.nih.gov/pubmed/33513840
http://dx.doi.org/10.3390/ma14030590
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author Lebedev, Alexander A.
Davydov, Sergey Yu
Eliseyev, Ilya A.
Roenkov, Alexander D.
Avdeev, Oleg
Lebedev, Sergey P.
Makarov, Yurii
Puzyk, Mikhail
Klotchenko, Sergey
Usikov, Alexander S.
author_facet Lebedev, Alexander A.
Davydov, Sergey Yu
Eliseyev, Ilya A.
Roenkov, Alexander D.
Avdeev, Oleg
Lebedev, Sergey P.
Makarov, Yurii
Puzyk, Mikhail
Klotchenko, Sergey
Usikov, Alexander S.
author_sort Lebedev, Alexander A.
collection PubMed
description This work is devoted to the development and optimization of the parameters of graphene-based sensors. The graphene films used in the present study were grown on semi-insulating 6H-SiC substrates by thermal decomposition of SiC at the temperature of ~1700 °C. The results of measurements by Auger and Raman spectroscopies confirmed the presence of single-layer graphene on the silicon carbide surface. Model approach to the theory of adsorption on epitaxial graphene is presented. It is demonstrated that the Green-function method in conjunction with the simple substrate models permit one to obtain analytical results for the charge transfer between adsorbed molecules and substrate. The sensor structure was formed on the graphene film by laser. Initially, a simpler gas sensor was made. The sensors developed in this study demonstrated sensitivity to the NO(2) concentration at the level of 1–0.01 ppb. The results obtained in the course of development and the results of testing of the graphene-based sensor for detection of protein molecules are also presented. The biosensor was fabricated by the technology previously developed for the gas sensor. The working capacity of the biosensor was tested with an immunochemical system constituted by fluorescein and monoclonal antibodies (mAbs) binding this dye.
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spelling pubmed-78659042021-02-07 Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization Lebedev, Alexander A. Davydov, Sergey Yu Eliseyev, Ilya A. Roenkov, Alexander D. Avdeev, Oleg Lebedev, Sergey P. Makarov, Yurii Puzyk, Mikhail Klotchenko, Sergey Usikov, Alexander S. Materials (Basel) Article This work is devoted to the development and optimization of the parameters of graphene-based sensors. The graphene films used in the present study were grown on semi-insulating 6H-SiC substrates by thermal decomposition of SiC at the temperature of ~1700 °C. The results of measurements by Auger and Raman spectroscopies confirmed the presence of single-layer graphene on the silicon carbide surface. Model approach to the theory of adsorption on epitaxial graphene is presented. It is demonstrated that the Green-function method in conjunction with the simple substrate models permit one to obtain analytical results for the charge transfer between adsorbed molecules and substrate. The sensor structure was formed on the graphene film by laser. Initially, a simpler gas sensor was made. The sensors developed in this study demonstrated sensitivity to the NO(2) concentration at the level of 1–0.01 ppb. The results obtained in the course of development and the results of testing of the graphene-based sensor for detection of protein molecules are also presented. The biosensor was fabricated by the technology previously developed for the gas sensor. The working capacity of the biosensor was tested with an immunochemical system constituted by fluorescein and monoclonal antibodies (mAbs) binding this dye. MDPI 2021-01-27 /pmc/articles/PMC7865904/ /pubmed/33513840 http://dx.doi.org/10.3390/ma14030590 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lebedev, Alexander A.
Davydov, Sergey Yu
Eliseyev, Ilya A.
Roenkov, Alexander D.
Avdeev, Oleg
Lebedev, Sergey P.
Makarov, Yurii
Puzyk, Mikhail
Klotchenko, Sergey
Usikov, Alexander S.
Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization
title Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization
title_full Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization
title_fullStr Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization
title_full_unstemmed Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization
title_short Graphene on SiC Substrate as Biosensor: Theoretical Background, Preparation, and Characterization
title_sort graphene on sic substrate as biosensor: theoretical background, preparation, and characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7865904/
https://www.ncbi.nlm.nih.gov/pubmed/33513840
http://dx.doi.org/10.3390/ma14030590
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