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Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock
Unlike in most eukaryotic cells, the genetic information of budding yeast in the exponential growth phase is only present in the form of decondensed chromatin, a configuration that does not allow its visualization in cell nuclei conventionally prepared for transmission electron microscopy. In this w...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866036/ https://www.ncbi.nlm.nih.gov/pubmed/33498839 http://dx.doi.org/10.3390/ijms22031132 |
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author | Thelen, Nicolas Defourny, Jean Lafontaine, Denis L. J. Thiry, Marc |
author_facet | Thelen, Nicolas Defourny, Jean Lafontaine, Denis L. J. Thiry, Marc |
author_sort | Thelen, Nicolas |
collection | PubMed |
description | Unlike in most eukaryotic cells, the genetic information of budding yeast in the exponential growth phase is only present in the form of decondensed chromatin, a configuration that does not allow its visualization in cell nuclei conventionally prepared for transmission electron microscopy. In this work, we studied the distribution of chromatin and its relationships to the nucleolus using different cytochemical and immunocytological approaches applied to yeast cells subjected to hyperosmotic shock. Our results show that osmotic shock induces the formation of heterochromatin patches in the nucleoplasm and intranucleolar regions of the yeast nucleus. In the nucleolus, we further revealed the presence of osmotic shock-resistant DNA in the fibrillar cords which, in places, take on a pinnate appearance reminiscent of ribosomal genes in active transcription as observed after molecular spreading (“Christmas trees”). We also identified chromatin-associated granules whose size, composition and behaviour after osmotic shock are reminiscent of that of mammalian perichromatin granules. Altogether, these data reveal that it is possible to visualize heterochromatin in yeast and suggest that the yeast nucleus displays a less-effective compartmentalized organization than that of mammals. |
format | Online Article Text |
id | pubmed-7866036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78660362021-02-07 Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock Thelen, Nicolas Defourny, Jean Lafontaine, Denis L. J. Thiry, Marc Int J Mol Sci Article Unlike in most eukaryotic cells, the genetic information of budding yeast in the exponential growth phase is only present in the form of decondensed chromatin, a configuration that does not allow its visualization in cell nuclei conventionally prepared for transmission electron microscopy. In this work, we studied the distribution of chromatin and its relationships to the nucleolus using different cytochemical and immunocytological approaches applied to yeast cells subjected to hyperosmotic shock. Our results show that osmotic shock induces the formation of heterochromatin patches in the nucleoplasm and intranucleolar regions of the yeast nucleus. In the nucleolus, we further revealed the presence of osmotic shock-resistant DNA in the fibrillar cords which, in places, take on a pinnate appearance reminiscent of ribosomal genes in active transcription as observed after molecular spreading (“Christmas trees”). We also identified chromatin-associated granules whose size, composition and behaviour after osmotic shock are reminiscent of that of mammalian perichromatin granules. Altogether, these data reveal that it is possible to visualize heterochromatin in yeast and suggest that the yeast nucleus displays a less-effective compartmentalized organization than that of mammals. MDPI 2021-01-24 /pmc/articles/PMC7866036/ /pubmed/33498839 http://dx.doi.org/10.3390/ijms22031132 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Thelen, Nicolas Defourny, Jean Lafontaine, Denis L. J. Thiry, Marc Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock |
title | Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock |
title_full | Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock |
title_fullStr | Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock |
title_full_unstemmed | Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock |
title_short | Visualization of Chromatin in the Yeast Nucleus and Nucleolus Using Hyperosmotic Shock |
title_sort | visualization of chromatin in the yeast nucleus and nucleolus using hyperosmotic shock |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866036/ https://www.ncbi.nlm.nih.gov/pubmed/33498839 http://dx.doi.org/10.3390/ijms22031132 |
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