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Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus

Proteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim to characterize the proteomic profile of gastric cancer (GC) in patients with diabetes mellitus (DM) type 2. Forty GC tissue samples including 19 cases from diabetic patients and 21 cases from indivi...

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Autores principales: Osório, Hugo, Silva, Cátia, Ferreira, Marta, Gullo, Irene, Máximo, Valdemar, Barros, Rita, Mendonça, Fernando, Oliveira, Carla, Carneiro, Fátima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866049/
https://www.ncbi.nlm.nih.gov/pubmed/33494396
http://dx.doi.org/10.3390/jcm10030407
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author Osório, Hugo
Silva, Cátia
Ferreira, Marta
Gullo, Irene
Máximo, Valdemar
Barros, Rita
Mendonça, Fernando
Oliveira, Carla
Carneiro, Fátima
author_facet Osório, Hugo
Silva, Cátia
Ferreira, Marta
Gullo, Irene
Máximo, Valdemar
Barros, Rita
Mendonça, Fernando
Oliveira, Carla
Carneiro, Fátima
author_sort Osório, Hugo
collection PubMed
description Proteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim to characterize the proteomic profile of gastric cancer (GC) in patients with diabetes mellitus (DM) type 2. Forty GC tissue samples including 19 cases from diabetic patients and 21 cases from individuals without diabetes (control group) were selected for the proteomics analysis. Gastric tissues were processed following the single-pot, solid-phase-enhanced sample preparation approach—SP3 and enzymatic digestion with trypsin. The resulting peptides were analyzed by LC-MS Liquid Chromatography—Mass Spectrometry (LC-MS). The comparison of protein expression levels between GC samples from diabetic and non-diabetic patients was performed by label-free quantification (LFQ). A total of 6599 protein groups were identified in the 40 samples. Thirty-seven proteins were differentially expressed among the two groups, with 16 upregulated and 21 downregulated in the diabetic cohort. Statistical overrepresentation tests were considered for different annotation sets including the Gene Ontology(GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, and Disease functional databases. Upregulated proteins in the GC samples from diabetic patients were particularly enriched in respiratory electron transport and alcohol metabolic biological processes, while downregulated proteins were associated with epithelial cancers, intestinal diseases, and cell–cell junction cellular components. Taken together, these results support the data already obtained by previous studies that associate diabetes with metabolic disorders and diabetes-associated diseases, such as Alzheimer’s and Parkinson’s, and also provide valuable insights into seven GC-associated protein targets, claudin-3, polymeric immunoglobulin receptor protein, cadherin-17, villin-1, transglutaminase-2, desmoglein-2, and mucin-13, which warrant further investigation.
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spelling pubmed-78660492021-02-07 Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus Osório, Hugo Silva, Cátia Ferreira, Marta Gullo, Irene Máximo, Valdemar Barros, Rita Mendonça, Fernando Oliveira, Carla Carneiro, Fátima J Clin Med Article Proteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim to characterize the proteomic profile of gastric cancer (GC) in patients with diabetes mellitus (DM) type 2. Forty GC tissue samples including 19 cases from diabetic patients and 21 cases from individuals without diabetes (control group) were selected for the proteomics analysis. Gastric tissues were processed following the single-pot, solid-phase-enhanced sample preparation approach—SP3 and enzymatic digestion with trypsin. The resulting peptides were analyzed by LC-MS Liquid Chromatography—Mass Spectrometry (LC-MS). The comparison of protein expression levels between GC samples from diabetic and non-diabetic patients was performed by label-free quantification (LFQ). A total of 6599 protein groups were identified in the 40 samples. Thirty-seven proteins were differentially expressed among the two groups, with 16 upregulated and 21 downregulated in the diabetic cohort. Statistical overrepresentation tests were considered for different annotation sets including the Gene Ontology(GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, and Disease functional databases. Upregulated proteins in the GC samples from diabetic patients were particularly enriched in respiratory electron transport and alcohol metabolic biological processes, while downregulated proteins were associated with epithelial cancers, intestinal diseases, and cell–cell junction cellular components. Taken together, these results support the data already obtained by previous studies that associate diabetes with metabolic disorders and diabetes-associated diseases, such as Alzheimer’s and Parkinson’s, and also provide valuable insights into seven GC-associated protein targets, claudin-3, polymeric immunoglobulin receptor protein, cadherin-17, villin-1, transglutaminase-2, desmoglein-2, and mucin-13, which warrant further investigation. MDPI 2021-01-21 /pmc/articles/PMC7866049/ /pubmed/33494396 http://dx.doi.org/10.3390/jcm10030407 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Osório, Hugo
Silva, Cátia
Ferreira, Marta
Gullo, Irene
Máximo, Valdemar
Barros, Rita
Mendonça, Fernando
Oliveira, Carla
Carneiro, Fátima
Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus
title Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus
title_full Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus
title_fullStr Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus
title_full_unstemmed Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus
title_short Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus
title_sort proteomics analysis of gastric cancer patients with diabetes mellitus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866049/
https://www.ncbi.nlm.nih.gov/pubmed/33494396
http://dx.doi.org/10.3390/jcm10030407
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