Cargando…

Radical Hemithoracic Radiotherapy Induces Systemic Metabolomics Changes That Are Associated with the Clinical Outcome of Malignant Pleural Mesothelioma Patients

SIMPLE SUMMARY: Radical hemithoracic radiotherapy represents a promising new advance in the field of radiation oncology and encouraging results have been achieved in the treatment of malignant pleural mesothelioma patients. This study showed that this radiotherapy modality produces significant chang...

Descripción completa

Detalles Bibliográficos
Autores principales: Di Gregorio, Emanuela, Miolo, Gianmaria, Saorin, Asia, Muraro, Elena, Cangemi, Michela, Revelant, Alberto, Minatel, Emilio, Trovò, Marco, Steffan, Agostino, Corona, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866164/
https://www.ncbi.nlm.nih.gov/pubmed/33572739
http://dx.doi.org/10.3390/cancers13030508
_version_ 1783648017451057152
author Di Gregorio, Emanuela
Miolo, Gianmaria
Saorin, Asia
Muraro, Elena
Cangemi, Michela
Revelant, Alberto
Minatel, Emilio
Trovò, Marco
Steffan, Agostino
Corona, Giuseppe
author_facet Di Gregorio, Emanuela
Miolo, Gianmaria
Saorin, Asia
Muraro, Elena
Cangemi, Michela
Revelant, Alberto
Minatel, Emilio
Trovò, Marco
Steffan, Agostino
Corona, Giuseppe
author_sort Di Gregorio, Emanuela
collection PubMed
description SIMPLE SUMMARY: Radical hemithoracic radiotherapy represents a promising new advance in the field of radiation oncology and encouraging results have been achieved in the treatment of malignant pleural mesothelioma patients. This study showed that this radiotherapy modality produces significant changes in serum metabolomics profile mainly affecting arginine and polyamine biosynthesis pathways. Interestingly, individual metabolomics alterations were found associated with the clinical overall survival outcome of the radiotherapy treatment. These results highlight metabolomics profile analysis as a powerful prognostic tool useful to better understand the mechanisms underlying the interpatients variability and to identify patients who may receive the best benefit from this specific radiotherapy treatment. ABSTRACT: Radical hemithoracic radiotherapy (RHRT) represents an advanced therapeutic option able to improve overall survival of malignant pleural mesothelioma patients. This study aims to investigate the systemic effects of this radiotherapy modality on the serum metabolome and their potential implications in determining the individual clinical outcome. Nineteen patients undergoing RHRT at the dose of 50 Gy in 25 fractions were enrolled. Serum targeted metabolomics profiles were investigated at baseline and the end of radiotherapy by liquid chromatography and tandem mass spectrometry. Univariate and multivariate OPLS-DA analyses were applied to study the serum metabolomics changes induced by RHRT while PLS regression analysis to evaluate the association between such changes and overall survival. RHRT was found to affect almost all investigated metabolites classes, in particular, the amino acids citrulline and taurine, the C14, C18:1 and C18:2 acyl-carnitines as well as the unsaturated long chain phosphatidylcholines PC ae 42:5, PC ae 44:5 and PC ae 44:6 were significantly decreased. The enrichment analysis showed arginine metabolism and the polyamine biosynthesis as the most perturbed pathways. Moreover, specific metabolic changes encompassing the amino acids and acyl-carnitines resulted in association with the clinical outcome accounting for about 60% of the interpatients overall survival variability. This study highlighted that RHRT can induce profound systemic metabolic effects some of which may have a significant prognostic value. The integration of metabolomics in the clinical assessment of the malignant pleural mesothelioma could be useful to better identify the patients who can achieve the best benefit from the RHRT treatment.
format Online
Article
Text
id pubmed-7866164
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78661642021-02-07 Radical Hemithoracic Radiotherapy Induces Systemic Metabolomics Changes That Are Associated with the Clinical Outcome of Malignant Pleural Mesothelioma Patients Di Gregorio, Emanuela Miolo, Gianmaria Saorin, Asia Muraro, Elena Cangemi, Michela Revelant, Alberto Minatel, Emilio Trovò, Marco Steffan, Agostino Corona, Giuseppe Cancers (Basel) Article SIMPLE SUMMARY: Radical hemithoracic radiotherapy represents a promising new advance in the field of radiation oncology and encouraging results have been achieved in the treatment of malignant pleural mesothelioma patients. This study showed that this radiotherapy modality produces significant changes in serum metabolomics profile mainly affecting arginine and polyamine biosynthesis pathways. Interestingly, individual metabolomics alterations were found associated with the clinical overall survival outcome of the radiotherapy treatment. These results highlight metabolomics profile analysis as a powerful prognostic tool useful to better understand the mechanisms underlying the interpatients variability and to identify patients who may receive the best benefit from this specific radiotherapy treatment. ABSTRACT: Radical hemithoracic radiotherapy (RHRT) represents an advanced therapeutic option able to improve overall survival of malignant pleural mesothelioma patients. This study aims to investigate the systemic effects of this radiotherapy modality on the serum metabolome and their potential implications in determining the individual clinical outcome. Nineteen patients undergoing RHRT at the dose of 50 Gy in 25 fractions were enrolled. Serum targeted metabolomics profiles were investigated at baseline and the end of radiotherapy by liquid chromatography and tandem mass spectrometry. Univariate and multivariate OPLS-DA analyses were applied to study the serum metabolomics changes induced by RHRT while PLS regression analysis to evaluate the association between such changes and overall survival. RHRT was found to affect almost all investigated metabolites classes, in particular, the amino acids citrulline and taurine, the C14, C18:1 and C18:2 acyl-carnitines as well as the unsaturated long chain phosphatidylcholines PC ae 42:5, PC ae 44:5 and PC ae 44:6 were significantly decreased. The enrichment analysis showed arginine metabolism and the polyamine biosynthesis as the most perturbed pathways. Moreover, specific metabolic changes encompassing the amino acids and acyl-carnitines resulted in association with the clinical outcome accounting for about 60% of the interpatients overall survival variability. This study highlighted that RHRT can induce profound systemic metabolic effects some of which may have a significant prognostic value. The integration of metabolomics in the clinical assessment of the malignant pleural mesothelioma could be useful to better identify the patients who can achieve the best benefit from the RHRT treatment. MDPI 2021-01-29 /pmc/articles/PMC7866164/ /pubmed/33572739 http://dx.doi.org/10.3390/cancers13030508 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Gregorio, Emanuela
Miolo, Gianmaria
Saorin, Asia
Muraro, Elena
Cangemi, Michela
Revelant, Alberto
Minatel, Emilio
Trovò, Marco
Steffan, Agostino
Corona, Giuseppe
Radical Hemithoracic Radiotherapy Induces Systemic Metabolomics Changes That Are Associated with the Clinical Outcome of Malignant Pleural Mesothelioma Patients
title Radical Hemithoracic Radiotherapy Induces Systemic Metabolomics Changes That Are Associated with the Clinical Outcome of Malignant Pleural Mesothelioma Patients
title_full Radical Hemithoracic Radiotherapy Induces Systemic Metabolomics Changes That Are Associated with the Clinical Outcome of Malignant Pleural Mesothelioma Patients
title_fullStr Radical Hemithoracic Radiotherapy Induces Systemic Metabolomics Changes That Are Associated with the Clinical Outcome of Malignant Pleural Mesothelioma Patients
title_full_unstemmed Radical Hemithoracic Radiotherapy Induces Systemic Metabolomics Changes That Are Associated with the Clinical Outcome of Malignant Pleural Mesothelioma Patients
title_short Radical Hemithoracic Radiotherapy Induces Systemic Metabolomics Changes That Are Associated with the Clinical Outcome of Malignant Pleural Mesothelioma Patients
title_sort radical hemithoracic radiotherapy induces systemic metabolomics changes that are associated with the clinical outcome of malignant pleural mesothelioma patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866164/
https://www.ncbi.nlm.nih.gov/pubmed/33572739
http://dx.doi.org/10.3390/cancers13030508
work_keys_str_mv AT digregorioemanuela radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT miologianmaria radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT saorinasia radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT muraroelena radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT cangemimichela radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT revelantalberto radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT minatelemilio radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT trovomarco radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT steffanagostino radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients
AT coronagiuseppe radicalhemithoracicradiotherapyinducessystemicmetabolomicschangesthatareassociatedwiththeclinicaloutcomeofmalignantpleuralmesotheliomapatients