Cargando…
New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium)
BACKGROUND: MADS-box transcription factors (TFs) are the key regulators of multiple developmental processes in plants; among them, a chrysanthemum MADS-box TF CmANR1 has been isolated and described as functioning in root development in response to high nitrate concentration signals. However, how CmA...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866475/ https://www.ncbi.nlm.nih.gov/pubmed/33549046 http://dx.doi.org/10.1186/s12870-021-02860-7 |
_version_ | 1783648084142587904 |
---|---|
author | Sun, Cui-Hui Wang, Jia-Hui Gu, Kai-Di Zhang, Peng Zhang, Xin-Yi Zheng, Cheng-Shu Hu, Da-Gang Ma, Fangfang |
author_facet | Sun, Cui-Hui Wang, Jia-Hui Gu, Kai-Di Zhang, Peng Zhang, Xin-Yi Zheng, Cheng-Shu Hu, Da-Gang Ma, Fangfang |
author_sort | Sun, Cui-Hui |
collection | PubMed |
description | BACKGROUND: MADS-box transcription factors (TFs) are the key regulators of multiple developmental processes in plants; among them, a chrysanthemum MADS-box TF CmANR1 has been isolated and described as functioning in root development in response to high nitrate concentration signals. However, how CmANR1 affects root and shoot development remains unclear. RESULTS: We report that CmANR1 plays a positive role in root system development in chrysanthemum throughout the developmental stages of in vitro tissue cultures. Metabolomics combined with transcriptomics assays show that CmANR1 promotes robust root system development by facilitating nitrate assimilation, and influencing the metabolic pathways of amino acid, glycolysis, and the tricarboxylic acid cycle (TCA) cycle. Also, we found that the expression levels of TFs associated with the nitrate signaling pathways, such as AGL8, AGL21, and LBD29, are significantly up-regulated in CmANR1-transgenic plants relative to the wild-type (WT) control; by contrast, the expression levels of RHD3-LIKE, LBD37, and GATA23 were significantly down-regulated. These results suggest that these nitrate signaling associated TFs are involved in CmANR1-modulated control of root development. In addition, CmANR1 also acts as a positive regulator to control shoot growth and development. CONCLUSIONS: These findings provide potential mechanisms of MADS-box TF CmANR1 modulation of root and shoot development, which occurs by regulating a series of nitrate signaling associated TFs, and influencing the metabolic pathways of amino acid and glycolysis, as well as TCA cycle and nitrate assimilation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-02860-7. |
format | Online Article Text |
id | pubmed-7866475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-78664752021-02-08 New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium) Sun, Cui-Hui Wang, Jia-Hui Gu, Kai-Di Zhang, Peng Zhang, Xin-Yi Zheng, Cheng-Shu Hu, Da-Gang Ma, Fangfang BMC Plant Biol Research Article BACKGROUND: MADS-box transcription factors (TFs) are the key regulators of multiple developmental processes in plants; among them, a chrysanthemum MADS-box TF CmANR1 has been isolated and described as functioning in root development in response to high nitrate concentration signals. However, how CmANR1 affects root and shoot development remains unclear. RESULTS: We report that CmANR1 plays a positive role in root system development in chrysanthemum throughout the developmental stages of in vitro tissue cultures. Metabolomics combined with transcriptomics assays show that CmANR1 promotes robust root system development by facilitating nitrate assimilation, and influencing the metabolic pathways of amino acid, glycolysis, and the tricarboxylic acid cycle (TCA) cycle. Also, we found that the expression levels of TFs associated with the nitrate signaling pathways, such as AGL8, AGL21, and LBD29, are significantly up-regulated in CmANR1-transgenic plants relative to the wild-type (WT) control; by contrast, the expression levels of RHD3-LIKE, LBD37, and GATA23 were significantly down-regulated. These results suggest that these nitrate signaling associated TFs are involved in CmANR1-modulated control of root development. In addition, CmANR1 also acts as a positive regulator to control shoot growth and development. CONCLUSIONS: These findings provide potential mechanisms of MADS-box TF CmANR1 modulation of root and shoot development, which occurs by regulating a series of nitrate signaling associated TFs, and influencing the metabolic pathways of amino acid and glycolysis, as well as TCA cycle and nitrate assimilation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-02860-7. BioMed Central 2021-02-06 /pmc/articles/PMC7866475/ /pubmed/33549046 http://dx.doi.org/10.1186/s12870-021-02860-7 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Sun, Cui-Hui Wang, Jia-Hui Gu, Kai-Di Zhang, Peng Zhang, Xin-Yi Zheng, Cheng-Shu Hu, Da-Gang Ma, Fangfang New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium) |
title | New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium) |
title_full | New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium) |
title_fullStr | New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium) |
title_full_unstemmed | New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium) |
title_short | New insights into the role of MADS-box transcription factor gene CmANR1 on root and shoot development in chrysanthemum (Chrysanthemum morifolium) |
title_sort | new insights into the role of mads-box transcription factor gene cmanr1 on root and shoot development in chrysanthemum (chrysanthemum morifolium) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866475/ https://www.ncbi.nlm.nih.gov/pubmed/33549046 http://dx.doi.org/10.1186/s12870-021-02860-7 |
work_keys_str_mv | AT suncuihui newinsightsintotheroleofmadsboxtranscriptionfactorgenecmanr1onrootandshootdevelopmentinchrysanthemumchrysanthemummorifolium AT wangjiahui newinsightsintotheroleofmadsboxtranscriptionfactorgenecmanr1onrootandshootdevelopmentinchrysanthemumchrysanthemummorifolium AT gukaidi newinsightsintotheroleofmadsboxtranscriptionfactorgenecmanr1onrootandshootdevelopmentinchrysanthemumchrysanthemummorifolium AT zhangpeng newinsightsintotheroleofmadsboxtranscriptionfactorgenecmanr1onrootandshootdevelopmentinchrysanthemumchrysanthemummorifolium AT zhangxinyi newinsightsintotheroleofmadsboxtranscriptionfactorgenecmanr1onrootandshootdevelopmentinchrysanthemumchrysanthemummorifolium AT zhengchengshu newinsightsintotheroleofmadsboxtranscriptionfactorgenecmanr1onrootandshootdevelopmentinchrysanthemumchrysanthemummorifolium AT hudagang newinsightsintotheroleofmadsboxtranscriptionfactorgenecmanr1onrootandshootdevelopmentinchrysanthemumchrysanthemummorifolium AT mafangfang newinsightsintotheroleofmadsboxtranscriptionfactorgenecmanr1onrootandshootdevelopmentinchrysanthemumchrysanthemummorifolium |