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Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillus helveticus in milk

The aim of the research was to explore the effect of carbon sources on the proliferation of Lactobacillus helveticus. In this study, lactose was added to skim milk medium and the effects of carbon sources on the growth and proliferation of Lactobacillus helveticus in low‐ and high‐sugar media were c...

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Detalles Bibliográficos
Autores principales: Zang, Jingyi, Wang, Tao, Piotr, Dziugan, Zhao, Hongfei, Zhang, Bolin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866610/
https://www.ncbi.nlm.nih.gov/pubmed/33598188
http://dx.doi.org/10.1002/fsn3.2076
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author Zang, Jingyi
Wang, Tao
Piotr, Dziugan
Zhao, Hongfei
Zhang, Bolin
author_facet Zang, Jingyi
Wang, Tao
Piotr, Dziugan
Zhao, Hongfei
Zhang, Bolin
author_sort Zang, Jingyi
collection PubMed
description The aim of the research was to explore the effect of carbon sources on the proliferation of Lactobacillus helveticus. In this study, lactose was added to skim milk medium and the effects of carbon sources on the growth and proliferation of Lactobacillus helveticus in low‐ and high‐sugar media were compared from the aspects of metabolism‐related enzyme activity, proteomics, and transcriptomics. The results showed that under high‐sugar conditions, the rate of substance transport to cells and the Embden–Meyerhof–Parnas (EMP) pathway were significantly accelerated. The synthesis and metabolism of cells were significantly enhanced, which was beneficial to the rapid proliferation of cells. By increasing the lactose concentration in the medium and optimizing the culture method, the cell density of Lactobacillus helveticus reached 3.98 × 10(9) CFU/ml; a good proliferation effect was obtained.
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spelling pubmed-78666102021-02-16 Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillus helveticus in milk Zang, Jingyi Wang, Tao Piotr, Dziugan Zhao, Hongfei Zhang, Bolin Food Sci Nutr Original Research The aim of the research was to explore the effect of carbon sources on the proliferation of Lactobacillus helveticus. In this study, lactose was added to skim milk medium and the effects of carbon sources on the growth and proliferation of Lactobacillus helveticus in low‐ and high‐sugar media were compared from the aspects of metabolism‐related enzyme activity, proteomics, and transcriptomics. The results showed that under high‐sugar conditions, the rate of substance transport to cells and the Embden–Meyerhof–Parnas (EMP) pathway were significantly accelerated. The synthesis and metabolism of cells were significantly enhanced, which was beneficial to the rapid proliferation of cells. By increasing the lactose concentration in the medium and optimizing the culture method, the cell density of Lactobacillus helveticus reached 3.98 × 10(9) CFU/ml; a good proliferation effect was obtained. John Wiley and Sons Inc. 2020-12-19 /pmc/articles/PMC7866610/ /pubmed/33598188 http://dx.doi.org/10.1002/fsn3.2076 Text en © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Zang, Jingyi
Wang, Tao
Piotr, Dziugan
Zhao, Hongfei
Zhang, Bolin
Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillus helveticus in milk
title Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillus helveticus in milk
title_full Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillus helveticus in milk
title_fullStr Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillus helveticus in milk
title_full_unstemmed Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillus helveticus in milk
title_short Increasing lactose concentration is a strategy to improve the proliferation of Lactobacillus helveticus in milk
title_sort increasing lactose concentration is a strategy to improve the proliferation of lactobacillus helveticus in milk
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866610/
https://www.ncbi.nlm.nih.gov/pubmed/33598188
http://dx.doi.org/10.1002/fsn3.2076
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