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Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H(2)O(2)-induced oxidative damage in RAW 264.7 cells

This work presents an investigation on the composition and structure of polysaccharides from the roots of Tetrastigma hemsleyanum (THP) and its associated antioxidant activity. It further explores the protective effect of THP on RAW264.7 cells against cytotoxicity induced by H(2)O(2). Ion chromatogr...

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Autores principales: Huang, Qi, He, Wen, Khudoyberdiev, Ilkhomjon, Ye, Chun-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866644/
https://www.ncbi.nlm.nih.gov/pubmed/33546740
http://dx.doi.org/10.1186/s13065-021-00738-1
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author Huang, Qi
He, Wen
Khudoyberdiev, Ilkhomjon
Ye, Chun-Lin
author_facet Huang, Qi
He, Wen
Khudoyberdiev, Ilkhomjon
Ye, Chun-Lin
author_sort Huang, Qi
collection PubMed
description This work presents an investigation on the composition and structure of polysaccharides from the roots of Tetrastigma hemsleyanum (THP) and its associated antioxidant activity. It further explores the protective effect of THP on RAW264.7 cells against cytotoxicity induced by H(2)O(2). Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. Furthermore, gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS) was employed to deduce the relative molecular mass (M(w)) of the polysaccharide, which was 177.1 ± 1.8 kDa. Fourier transform infrared spectroscopy (FT-IR) and Congo red binding assay highlighted that the THP had a steady α-triple helix conformation. Similarly, assays of antioxidant activity disclosed that THP had reasonable concentration-dependent hydroxyl radical and superoxide radical scavenging activities, peroxidation inhibition ability and ferrous ion chelating potency, in addition to a significant 1,1-diphenyl-2-picrylhydrazyl radical scavenging capacity. Moreover, THP could protect RAW264.7 cells against H(2)O(2)-induced cytotoxicity by decreasing intracellular ROS levels, reducing catalase (CAT) and superoxide dismutase (SOD) activities, increasing lactate dehydrogenase (LDH) activity and increment in malondialdehyde (MDA) level. Data retrieved from the in vitro models explicitly established the antioxidant capability of polysaccharides from T. hemsleyanum root extracts.
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spelling pubmed-78666442021-02-08 Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H(2)O(2)-induced oxidative damage in RAW 264.7 cells Huang, Qi He, Wen Khudoyberdiev, Ilkhomjon Ye, Chun-Lin BMC Chem Research Article This work presents an investigation on the composition and structure of polysaccharides from the roots of Tetrastigma hemsleyanum (THP) and its associated antioxidant activity. It further explores the protective effect of THP on RAW264.7 cells against cytotoxicity induced by H(2)O(2). Ion chromatography (IC) revealed that THP contained glucose, arabinose, mannose, glucuronic acid, galactose and galacturonic acid, in different molar ratios. Furthermore, gel permeation chromatography-refractive index-multiangle laser light scattering (GPC-RI-MALS) was employed to deduce the relative molecular mass (M(w)) of the polysaccharide, which was 177.1 ± 1.8 kDa. Fourier transform infrared spectroscopy (FT-IR) and Congo red binding assay highlighted that the THP had a steady α-triple helix conformation. Similarly, assays of antioxidant activity disclosed that THP had reasonable concentration-dependent hydroxyl radical and superoxide radical scavenging activities, peroxidation inhibition ability and ferrous ion chelating potency, in addition to a significant 1,1-diphenyl-2-picrylhydrazyl radical scavenging capacity. Moreover, THP could protect RAW264.7 cells against H(2)O(2)-induced cytotoxicity by decreasing intracellular ROS levels, reducing catalase (CAT) and superoxide dismutase (SOD) activities, increasing lactate dehydrogenase (LDH) activity and increment in malondialdehyde (MDA) level. Data retrieved from the in vitro models explicitly established the antioxidant capability of polysaccharides from T. hemsleyanum root extracts. Springer International Publishing 2021-02-05 /pmc/articles/PMC7866644/ /pubmed/33546740 http://dx.doi.org/10.1186/s13065-021-00738-1 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Huang, Qi
He, Wen
Khudoyberdiev, Ilkhomjon
Ye, Chun-Lin
Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H(2)O(2)-induced oxidative damage in RAW 264.7 cells
title Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H(2)O(2)-induced oxidative damage in RAW 264.7 cells
title_full Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H(2)O(2)-induced oxidative damage in RAW 264.7 cells
title_fullStr Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H(2)O(2)-induced oxidative damage in RAW 264.7 cells
title_full_unstemmed Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H(2)O(2)-induced oxidative damage in RAW 264.7 cells
title_short Characterization of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg Roots and their effects on antioxidant activity and H(2)O(2)-induced oxidative damage in RAW 264.7 cells
title_sort characterization of polysaccharides from tetrastigma hemsleyanum diels et gilg roots and their effects on antioxidant activity and h(2)o(2)-induced oxidative damage in raw 264.7 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866644/
https://www.ncbi.nlm.nih.gov/pubmed/33546740
http://dx.doi.org/10.1186/s13065-021-00738-1
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