Cargando…
Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae
BACKGROUND: The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout anim...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866777/ https://www.ncbi.nlm.nih.gov/pubmed/33546586 http://dx.doi.org/10.1186/s12870-021-02854-5 |
_version_ | 1783648152241307648 |
---|---|
author | Moran-Bertot, Ivis Rodríguez-Cabrera, Lianet Borras-Hidalgo, Orlando Huang, Siliang Kan, Yunchao Wright, Denis J. Ayra-Pardo, Camilo |
author_facet | Moran-Bertot, Ivis Rodríguez-Cabrera, Lianet Borras-Hidalgo, Orlando Huang, Siliang Kan, Yunchao Wright, Denis J. Ayra-Pardo, Camilo |
author_sort | Moran-Bertot, Ivis |
collection | PubMed |
description | BACKGROUND: The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae. RESULTS: The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls. CONCLUSIONS: We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-02854-5. |
format | Online Article Text |
id | pubmed-7866777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-78667772021-02-08 Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae Moran-Bertot, Ivis Rodríguez-Cabrera, Lianet Borras-Hidalgo, Orlando Huang, Siliang Kan, Yunchao Wright, Denis J. Ayra-Pardo, Camilo BMC Plant Biol Research Article BACKGROUND: The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae. RESULTS: The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls. CONCLUSIONS: We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-02854-5. BioMed Central 2021-02-05 /pmc/articles/PMC7866777/ /pubmed/33546586 http://dx.doi.org/10.1186/s12870-021-02854-5 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Moran-Bertot, Ivis Rodríguez-Cabrera, Lianet Borras-Hidalgo, Orlando Huang, Siliang Kan, Yunchao Wright, Denis J. Ayra-Pardo, Camilo Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae |
title | Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae |
title_full | Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae |
title_fullStr | Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae |
title_full_unstemmed | Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae |
title_short | Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae |
title_sort | potato virus x-mediated constitutive expression of plutella xylostella pxsdf2l1 gene in nicotiana benthamiana confers resistance to phytophthora parasitica var. nicotianae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866777/ https://www.ncbi.nlm.nih.gov/pubmed/33546586 http://dx.doi.org/10.1186/s12870-021-02854-5 |
work_keys_str_mv | AT moranbertotivis potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae AT rodriguezcabreralianet potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae AT borrashidalgoorlando potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae AT huangsiliang potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae AT kanyunchao potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae AT wrightdenisj potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae AT ayrapardocamilo potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae |