Cargando…

Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae

BACKGROUND: The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout anim...

Descripción completa

Detalles Bibliográficos
Autores principales: Moran-Bertot, Ivis, Rodríguez-Cabrera, Lianet, Borras-Hidalgo, Orlando, Huang, Siliang, Kan, Yunchao, Wright, Denis J., Ayra-Pardo, Camilo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866777/
https://www.ncbi.nlm.nih.gov/pubmed/33546586
http://dx.doi.org/10.1186/s12870-021-02854-5
_version_ 1783648152241307648
author Moran-Bertot, Ivis
Rodríguez-Cabrera, Lianet
Borras-Hidalgo, Orlando
Huang, Siliang
Kan, Yunchao
Wright, Denis J.
Ayra-Pardo, Camilo
author_facet Moran-Bertot, Ivis
Rodríguez-Cabrera, Lianet
Borras-Hidalgo, Orlando
Huang, Siliang
Kan, Yunchao
Wright, Denis J.
Ayra-Pardo, Camilo
author_sort Moran-Bertot, Ivis
collection PubMed
description BACKGROUND: The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae. RESULTS: The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls. CONCLUSIONS: We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-02854-5.
format Online
Article
Text
id pubmed-7866777
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-78667772021-02-08 Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae Moran-Bertot, Ivis Rodríguez-Cabrera, Lianet Borras-Hidalgo, Orlando Huang, Siliang Kan, Yunchao Wright, Denis J. Ayra-Pardo, Camilo BMC Plant Biol Research Article BACKGROUND: The Plutella xylostella PxSDF2L1 gene was previously reported to enhance insect resistance to pathogen at high basal transcription rate. PxSDF2L1 shows similitude with the stromal cell-derived factor 2 (SDF2), an ER stress-induced chaperon protein that is highly conserved throughout animals and plants. The precise biological function of SDF2 is not clear, but its expression is required for innate immunity in plants. Here, we investigate whether a continuous expression of PxSDF2L1 in Nicotiana benthamiana can similarly confer resistance to plant pathogen, particularly, the black shank Phytophthora parasitica var. nicotianae. RESULTS: The N. benthamiana plants were inoculated with agrobacteria transformed with a PVX-based binary vector carrying the PxSDF2L1 gene; similar agroinoculation experiments with a PVX vector carrying the GFP gene were used for controls. In pot trials, agroinfected N. benthamiana plants constitutively expressing PxSDF2L1 showed a significant reduction of stem disease symptoms caused by the inoculation with P. parasitica, compared with controls. CONCLUSIONS: We confirm a role of PxSDF2L1 in resistance to black shank, with a potential application to engineering active resistance against this oomycete in the commercial N. tabacum species and propose its evaluation in other crop families and plant pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-02854-5. BioMed Central 2021-02-05 /pmc/articles/PMC7866777/ /pubmed/33546586 http://dx.doi.org/10.1186/s12870-021-02854-5 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Moran-Bertot, Ivis
Rodríguez-Cabrera, Lianet
Borras-Hidalgo, Orlando
Huang, Siliang
Kan, Yunchao
Wright, Denis J.
Ayra-Pardo, Camilo
Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae
title Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae
title_full Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae
title_fullStr Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae
title_full_unstemmed Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae
title_short Potato virus X-mediated constitutive expression of Plutella xylostella PxSDF2L1 gene in Nicotiana benthamiana confers resistance to Phytophthora parasitica var. nicotianae
title_sort potato virus x-mediated constitutive expression of plutella xylostella pxsdf2l1 gene in nicotiana benthamiana confers resistance to phytophthora parasitica var. nicotianae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866777/
https://www.ncbi.nlm.nih.gov/pubmed/33546586
http://dx.doi.org/10.1186/s12870-021-02854-5
work_keys_str_mv AT moranbertotivis potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae
AT rodriguezcabreralianet potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae
AT borrashidalgoorlando potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae
AT huangsiliang potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae
AT kanyunchao potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae
AT wrightdenisj potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae
AT ayrapardocamilo potatovirusxmediatedconstitutiveexpressionofplutellaxylostellapxsdf2l1geneinnicotianabenthamianaconfersresistancetophytophthoraparasiticavarnicotianae