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Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts

This study investigates the effect of graphene scaffold on morphology, viability, cytoskeleton, focal contacts, mitochondrial network morphology and activity in BALB/3T3 fibroblasts and provides new data on biocompatibility of the “graphene-family nanomaterials”. We used graphene monolayer applied o...

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Autores principales: Lasocka, Iwona, Szulc-Dąbrowska, Lidia, Skibniewski, Michał, Skibniewska, Ewa, Gregorczyk-Zboroch, Karolina, Pasternak, Iwona, Hubalek Kalbacova, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866834/
https://www.ncbi.nlm.nih.gov/pubmed/33573304
http://dx.doi.org/10.3390/ma14030643
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author Lasocka, Iwona
Szulc-Dąbrowska, Lidia
Skibniewski, Michał
Skibniewska, Ewa
Gregorczyk-Zboroch, Karolina
Pasternak, Iwona
Hubalek Kalbacova, Marie
author_facet Lasocka, Iwona
Szulc-Dąbrowska, Lidia
Skibniewski, Michał
Skibniewska, Ewa
Gregorczyk-Zboroch, Karolina
Pasternak, Iwona
Hubalek Kalbacova, Marie
author_sort Lasocka, Iwona
collection PubMed
description This study investigates the effect of graphene scaffold on morphology, viability, cytoskeleton, focal contacts, mitochondrial network morphology and activity in BALB/3T3 fibroblasts and provides new data on biocompatibility of the “graphene-family nanomaterials”. We used graphene monolayer applied onto glass cover slide by electrochemical delamination method and regular glass cover slide, as a reference. The morphology of fibroblasts growing on graphene was unaltered, and the cell viability was 95% compared to control cells on non-coated glass slide. There was no significant difference in the cell size (spreading) between both groups studied. Graphene platform significantly increased BALB/3T3 cell mitochondrial activity (WST-8 test) compared to glass substrate. To demonstrate the variability in focal contacts pattern, the effect of graphene on vinculin was examined, which revealed a significant increase in focal contact size comparing to control-glass slide. There was no disruption in mitochondrial network morphology, which was branched and well connected in relation to the control group. Evaluation of the JC-1 red/green fluorescence intensity ratio revealed similar levels of mitochondrial membrane potential in cells growing on graphene-coated and uncoated slides. These results indicate that graphene monolayer scaffold is cytocompatible with connective tissue cells examined and could be beneficial for tissue engineering therapy.
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spelling pubmed-78668342021-02-07 Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts Lasocka, Iwona Szulc-Dąbrowska, Lidia Skibniewski, Michał Skibniewska, Ewa Gregorczyk-Zboroch, Karolina Pasternak, Iwona Hubalek Kalbacova, Marie Materials (Basel) Article This study investigates the effect of graphene scaffold on morphology, viability, cytoskeleton, focal contacts, mitochondrial network morphology and activity in BALB/3T3 fibroblasts and provides new data on biocompatibility of the “graphene-family nanomaterials”. We used graphene monolayer applied onto glass cover slide by electrochemical delamination method and regular glass cover slide, as a reference. The morphology of fibroblasts growing on graphene was unaltered, and the cell viability was 95% compared to control cells on non-coated glass slide. There was no significant difference in the cell size (spreading) between both groups studied. Graphene platform significantly increased BALB/3T3 cell mitochondrial activity (WST-8 test) compared to glass substrate. To demonstrate the variability in focal contacts pattern, the effect of graphene on vinculin was examined, which revealed a significant increase in focal contact size comparing to control-glass slide. There was no disruption in mitochondrial network morphology, which was branched and well connected in relation to the control group. Evaluation of the JC-1 red/green fluorescence intensity ratio revealed similar levels of mitochondrial membrane potential in cells growing on graphene-coated and uncoated slides. These results indicate that graphene monolayer scaffold is cytocompatible with connective tissue cells examined and could be beneficial for tissue engineering therapy. MDPI 2021-01-30 /pmc/articles/PMC7866834/ /pubmed/33573304 http://dx.doi.org/10.3390/ma14030643 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lasocka, Iwona
Szulc-Dąbrowska, Lidia
Skibniewski, Michał
Skibniewska, Ewa
Gregorczyk-Zboroch, Karolina
Pasternak, Iwona
Hubalek Kalbacova, Marie
Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts
title Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts
title_full Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts
title_fullStr Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts
title_full_unstemmed Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts
title_short Cytocompatibility of Graphene Monolayer and Its Impact on Focal Cell Adhesion, Mitochondrial Morphology and Activity in BALB/3T3 Fibroblasts
title_sort cytocompatibility of graphene monolayer and its impact on focal cell adhesion, mitochondrial morphology and activity in balb/3t3 fibroblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866834/
https://www.ncbi.nlm.nih.gov/pubmed/33573304
http://dx.doi.org/10.3390/ma14030643
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