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NMR Structure Determinations of Small Proteins Using only One Fractionally 20% (13)C- and Uniformly 100% (15)N-Labeled Sample

Uniformly (13)C- and (15)N-labeled samples ensure fast and reliable nuclear magnetic resonance (NMR) assignments of proteins and are commonly used for structure elucidation by NMR. However, the preparation of uniformly labeled samples is a labor-intensive and expensive step. Reducing the portion of...

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Detalles Bibliográficos
Autores principales: Heikkinen, Harri A., Backlund, Sofia M., Iwaï, Hideo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867066/
https://www.ncbi.nlm.nih.gov/pubmed/33535444
http://dx.doi.org/10.3390/molecules26030747