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Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor

In bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use...

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Autores principales: Ramírez-Rodríguez, Gloria Belén, Pereira, Ana Rita, Herrmann, Marietta, Hansmann, Jan, Delgado-López, José Manuel, Sprio, Simone, Tampieri, Anna, Sandri, Monica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867135/
https://www.ncbi.nlm.nih.gov/pubmed/33535576
http://dx.doi.org/10.3390/ijms22031447
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author Ramírez-Rodríguez, Gloria Belén
Pereira, Ana Rita
Herrmann, Marietta
Hansmann, Jan
Delgado-López, José Manuel
Sprio, Simone
Tampieri, Anna
Sandri, Monica
author_facet Ramírez-Rodríguez, Gloria Belén
Pereira, Ana Rita
Herrmann, Marietta
Hansmann, Jan
Delgado-López, José Manuel
Sprio, Simone
Tampieri, Anna
Sandri, Monica
author_sort Ramírez-Rodríguez, Gloria Belén
collection PubMed
description In bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to simultaneously provide biochemical and biophysical cues guiding stem cell fate. The scaffolds were fabricated by mineralization of recombinant collagen in the presence of magnesium (RCP.MgAp). The organic matrix was homogeneously mineralized with apatite nanocrystals, similar in composition to those found in bone. X-Ray microtomography images revealed isotropic porous structure with optimum porosity for cell ingrowth. In fact, an optimal cell repopulation through the entire scaffolds was obtained after 1 day of dynamic seeding in the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited higher cell viability and a clear trend of up-regulation of osteogenic genes than control (non-mineralized) scaffolds. Results demonstrate the potential of the combination of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic culture of hMSC as a promising strategy to closely mimic bone ECM.
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spelling pubmed-78671352021-02-07 Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor Ramírez-Rodríguez, Gloria Belén Pereira, Ana Rita Herrmann, Marietta Hansmann, Jan Delgado-López, José Manuel Sprio, Simone Tampieri, Anna Sandri, Monica Int J Mol Sci Article In bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to simultaneously provide biochemical and biophysical cues guiding stem cell fate. The scaffolds were fabricated by mineralization of recombinant collagen in the presence of magnesium (RCP.MgAp). The organic matrix was homogeneously mineralized with apatite nanocrystals, similar in composition to those found in bone. X-Ray microtomography images revealed isotropic porous structure with optimum porosity for cell ingrowth. In fact, an optimal cell repopulation through the entire scaffolds was obtained after 1 day of dynamic seeding in the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited higher cell viability and a clear trend of up-regulation of osteogenic genes than control (non-mineralized) scaffolds. Results demonstrate the potential of the combination of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic culture of hMSC as a promising strategy to closely mimic bone ECM. MDPI 2021-02-01 /pmc/articles/PMC7867135/ /pubmed/33535576 http://dx.doi.org/10.3390/ijms22031447 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramírez-Rodríguez, Gloria Belén
Pereira, Ana Rita
Herrmann, Marietta
Hansmann, Jan
Delgado-López, José Manuel
Sprio, Simone
Tampieri, Anna
Sandri, Monica
Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
title Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
title_full Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
title_fullStr Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
title_full_unstemmed Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
title_short Biomimetic Mineralization Promotes Viability and Differentiation of Human Mesenchymal Stem Cells in a Perfusion Bioreactor
title_sort biomimetic mineralization promotes viability and differentiation of human mesenchymal stem cells in a perfusion bioreactor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867135/
https://www.ncbi.nlm.nih.gov/pubmed/33535576
http://dx.doi.org/10.3390/ijms22031447
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