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Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study

Scaffolds of polycaprolactone-30% hydroxyapatite (PCL-30% HA) were fabricated using melt stretching and multilayer deposition (MSMD), and the in vitro response of osteoblasts to the scaffolds was assessed. In group A, the scaffolds were immersed in 10 µg/mL bone morphogenetic protein-2 (BMP-2) solut...

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Autores principales: Rittipakorn, Pawornwan, Thuaksuban, Nuttawut, Mai-ngam, Katanchalee, Charoenla, Satrawut, Noppakunmongkolchai, Warobon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867198/
https://www.ncbi.nlm.nih.gov/pubmed/33535638
http://dx.doi.org/10.3390/polym13030466
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author Rittipakorn, Pawornwan
Thuaksuban, Nuttawut
Mai-ngam, Katanchalee
Charoenla, Satrawut
Noppakunmongkolchai, Warobon
author_facet Rittipakorn, Pawornwan
Thuaksuban, Nuttawut
Mai-ngam, Katanchalee
Charoenla, Satrawut
Noppakunmongkolchai, Warobon
author_sort Rittipakorn, Pawornwan
collection PubMed
description Scaffolds of polycaprolactone-30% hydroxyapatite (PCL-30% HA) were fabricated using melt stretching and multilayer deposition (MSMD), and the in vitro response of osteoblasts to the scaffolds was assessed. In group A, the scaffolds were immersed in 10 µg/mL bone morphogenetic protein-2 (BMP-2) solution prior to being seeded with osteoblasts, and they were cultured in the medium without BMP-2. In group B, the cell-scaffold constructs without BMP-2 were cultured in medium containing 10 µg/mL BMP-2. The results showed greater cell proliferation in group A. The upregulation of runt-related transcription factor 2 and osteocalcin genes correlated with the release of BMP-2 from the scaffolds. The PCL-30% HA MSMD scaffolds appear to be suitable for use as osteoconductive frameworks and BMP-2 carriers.
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spelling pubmed-78671982021-02-07 Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study Rittipakorn, Pawornwan Thuaksuban, Nuttawut Mai-ngam, Katanchalee Charoenla, Satrawut Noppakunmongkolchai, Warobon Polymers (Basel) Article Scaffolds of polycaprolactone-30% hydroxyapatite (PCL-30% HA) were fabricated using melt stretching and multilayer deposition (MSMD), and the in vitro response of osteoblasts to the scaffolds was assessed. In group A, the scaffolds were immersed in 10 µg/mL bone morphogenetic protein-2 (BMP-2) solution prior to being seeded with osteoblasts, and they were cultured in the medium without BMP-2. In group B, the cell-scaffold constructs without BMP-2 were cultured in medium containing 10 µg/mL BMP-2. The results showed greater cell proliferation in group A. The upregulation of runt-related transcription factor 2 and osteocalcin genes correlated with the release of BMP-2 from the scaffolds. The PCL-30% HA MSMD scaffolds appear to be suitable for use as osteoconductive frameworks and BMP-2 carriers. MDPI 2021-02-01 /pmc/articles/PMC7867198/ /pubmed/33535638 http://dx.doi.org/10.3390/polym13030466 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rittipakorn, Pawornwan
Thuaksuban, Nuttawut
Mai-ngam, Katanchalee
Charoenla, Satrawut
Noppakunmongkolchai, Warobon
Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study
title Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study
title_full Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study
title_fullStr Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study
title_full_unstemmed Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study
title_short Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study
title_sort bioactivity of a novel polycaprolactone-hydroxyapatite scaffold used as a carrier of low dose bmp-2: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867198/
https://www.ncbi.nlm.nih.gov/pubmed/33535638
http://dx.doi.org/10.3390/polym13030466
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