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The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress

Restraint stress causes various maternal diseases during pregnancy. β(2)-Adrenergic receptor (β(2)-AR) and Forkhead transcription factor class O 1 (FOXO1) are critical factors not only in stress, but also in reproduction. However, the role of FOXO1 in restraint stress, causing changes in the β(2)-AR...

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Autores principales: Lu, Jiayin, Chen, Yaoxing, Wang, Zixu, Cao, Jing, Dong, Yulan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867244/
https://www.ncbi.nlm.nih.gov/pubmed/33540675
http://dx.doi.org/10.3390/ijms22031478
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author Lu, Jiayin
Chen, Yaoxing
Wang, Zixu
Cao, Jing
Dong, Yulan
author_facet Lu, Jiayin
Chen, Yaoxing
Wang, Zixu
Cao, Jing
Dong, Yulan
author_sort Lu, Jiayin
collection PubMed
description Restraint stress causes various maternal diseases during pregnancy. β(2)-Adrenergic receptor (β(2)-AR) and Forkhead transcription factor class O 1 (FOXO1) are critical factors not only in stress, but also in reproduction. However, the role of FOXO1 in restraint stress, causing changes in the β(2)-AR pathway in pregnant mice, has been unclear. The aim of this research was to investigate the β(2)-AR pathway of restraint stress and its impact on the oxidative stress of the maternal uterus. In the study, maternal mice were treated with restraint stress by being restrained in a transparent and ventilated device before sacrifice on Pregnancy Day 5 (P5), Pregnancy Day 10 (P10), Pregnancy Day 15 (P15), and Pregnancy Day 20 (P20) as well as on Non-Pregnancy Day 5 (NP5). Restraint stress augmented blood corticosterone (CORT), norepinephrine (NE), and blood glucose levels, while oestradiol (E2) levels decreased. Moreover, restraint stress increased the mRNA levels of the FOXO family, β(2)-AR, and even the protein levels of FOXO1 and β(2)-AR in the uterus and ovaries. Furthermore, restraint stress increased uterine oxidative stress level. In vitro, the protein levels of FOXO1 were also obviously increased when β(2)-AR was activated in endometrial stromal cells (ESCs). In addition, phosphorylated-nuclear factor kappa-B p65 (p-NF-κB p65) and its target genes decreased significantly when FOXO1 was inhibited. Overall, it can be said that the β(2)-AR/FOXO1/p-NF-κB p65 pathway was activated when pregnant mice were under restraint stress. This study provides a scientific basis for the origin of psychological stress in pregnant women.
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spelling pubmed-78672442021-02-07 The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress Lu, Jiayin Chen, Yaoxing Wang, Zixu Cao, Jing Dong, Yulan Int J Mol Sci Article Restraint stress causes various maternal diseases during pregnancy. β(2)-Adrenergic receptor (β(2)-AR) and Forkhead transcription factor class O 1 (FOXO1) are critical factors not only in stress, but also in reproduction. However, the role of FOXO1 in restraint stress, causing changes in the β(2)-AR pathway in pregnant mice, has been unclear. The aim of this research was to investigate the β(2)-AR pathway of restraint stress and its impact on the oxidative stress of the maternal uterus. In the study, maternal mice were treated with restraint stress by being restrained in a transparent and ventilated device before sacrifice on Pregnancy Day 5 (P5), Pregnancy Day 10 (P10), Pregnancy Day 15 (P15), and Pregnancy Day 20 (P20) as well as on Non-Pregnancy Day 5 (NP5). Restraint stress augmented blood corticosterone (CORT), norepinephrine (NE), and blood glucose levels, while oestradiol (E2) levels decreased. Moreover, restraint stress increased the mRNA levels of the FOXO family, β(2)-AR, and even the protein levels of FOXO1 and β(2)-AR in the uterus and ovaries. Furthermore, restraint stress increased uterine oxidative stress level. In vitro, the protein levels of FOXO1 were also obviously increased when β(2)-AR was activated in endometrial stromal cells (ESCs). In addition, phosphorylated-nuclear factor kappa-B p65 (p-NF-κB p65) and its target genes decreased significantly when FOXO1 was inhibited. Overall, it can be said that the β(2)-AR/FOXO1/p-NF-κB p65 pathway was activated when pregnant mice were under restraint stress. This study provides a scientific basis for the origin of psychological stress in pregnant women. MDPI 2021-02-02 /pmc/articles/PMC7867244/ /pubmed/33540675 http://dx.doi.org/10.3390/ijms22031478 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Jiayin
Chen, Yaoxing
Wang, Zixu
Cao, Jing
Dong, Yulan
The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress
title The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress
title_full The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress
title_fullStr The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress
title_full_unstemmed The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress
title_short The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress
title_sort role of the foxo1/β(2)-ar/p-nf-κb p65 pathway in the development of endometrial stromal cells in pregnant mice under restraint stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867244/
https://www.ncbi.nlm.nih.gov/pubmed/33540675
http://dx.doi.org/10.3390/ijms22031478
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