Cargando…
The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress
Restraint stress causes various maternal diseases during pregnancy. β(2)-Adrenergic receptor (β(2)-AR) and Forkhead transcription factor class O 1 (FOXO1) are critical factors not only in stress, but also in reproduction. However, the role of FOXO1 in restraint stress, causing changes in the β(2)-AR...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867244/ https://www.ncbi.nlm.nih.gov/pubmed/33540675 http://dx.doi.org/10.3390/ijms22031478 |
_version_ | 1783648257930428416 |
---|---|
author | Lu, Jiayin Chen, Yaoxing Wang, Zixu Cao, Jing Dong, Yulan |
author_facet | Lu, Jiayin Chen, Yaoxing Wang, Zixu Cao, Jing Dong, Yulan |
author_sort | Lu, Jiayin |
collection | PubMed |
description | Restraint stress causes various maternal diseases during pregnancy. β(2)-Adrenergic receptor (β(2)-AR) and Forkhead transcription factor class O 1 (FOXO1) are critical factors not only in stress, but also in reproduction. However, the role of FOXO1 in restraint stress, causing changes in the β(2)-AR pathway in pregnant mice, has been unclear. The aim of this research was to investigate the β(2)-AR pathway of restraint stress and its impact on the oxidative stress of the maternal uterus. In the study, maternal mice were treated with restraint stress by being restrained in a transparent and ventilated device before sacrifice on Pregnancy Day 5 (P5), Pregnancy Day 10 (P10), Pregnancy Day 15 (P15), and Pregnancy Day 20 (P20) as well as on Non-Pregnancy Day 5 (NP5). Restraint stress augmented blood corticosterone (CORT), norepinephrine (NE), and blood glucose levels, while oestradiol (E2) levels decreased. Moreover, restraint stress increased the mRNA levels of the FOXO family, β(2)-AR, and even the protein levels of FOXO1 and β(2)-AR in the uterus and ovaries. Furthermore, restraint stress increased uterine oxidative stress level. In vitro, the protein levels of FOXO1 were also obviously increased when β(2)-AR was activated in endometrial stromal cells (ESCs). In addition, phosphorylated-nuclear factor kappa-B p65 (p-NF-κB p65) and its target genes decreased significantly when FOXO1 was inhibited. Overall, it can be said that the β(2)-AR/FOXO1/p-NF-κB p65 pathway was activated when pregnant mice were under restraint stress. This study provides a scientific basis for the origin of psychological stress in pregnant women. |
format | Online Article Text |
id | pubmed-7867244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78672442021-02-07 The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress Lu, Jiayin Chen, Yaoxing Wang, Zixu Cao, Jing Dong, Yulan Int J Mol Sci Article Restraint stress causes various maternal diseases during pregnancy. β(2)-Adrenergic receptor (β(2)-AR) and Forkhead transcription factor class O 1 (FOXO1) are critical factors not only in stress, but also in reproduction. However, the role of FOXO1 in restraint stress, causing changes in the β(2)-AR pathway in pregnant mice, has been unclear. The aim of this research was to investigate the β(2)-AR pathway of restraint stress and its impact on the oxidative stress of the maternal uterus. In the study, maternal mice were treated with restraint stress by being restrained in a transparent and ventilated device before sacrifice on Pregnancy Day 5 (P5), Pregnancy Day 10 (P10), Pregnancy Day 15 (P15), and Pregnancy Day 20 (P20) as well as on Non-Pregnancy Day 5 (NP5). Restraint stress augmented blood corticosterone (CORT), norepinephrine (NE), and blood glucose levels, while oestradiol (E2) levels decreased. Moreover, restraint stress increased the mRNA levels of the FOXO family, β(2)-AR, and even the protein levels of FOXO1 and β(2)-AR in the uterus and ovaries. Furthermore, restraint stress increased uterine oxidative stress level. In vitro, the protein levels of FOXO1 were also obviously increased when β(2)-AR was activated in endometrial stromal cells (ESCs). In addition, phosphorylated-nuclear factor kappa-B p65 (p-NF-κB p65) and its target genes decreased significantly when FOXO1 was inhibited. Overall, it can be said that the β(2)-AR/FOXO1/p-NF-κB p65 pathway was activated when pregnant mice were under restraint stress. This study provides a scientific basis for the origin of psychological stress in pregnant women. MDPI 2021-02-02 /pmc/articles/PMC7867244/ /pubmed/33540675 http://dx.doi.org/10.3390/ijms22031478 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lu, Jiayin Chen, Yaoxing Wang, Zixu Cao, Jing Dong, Yulan The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress |
title | The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress |
title_full | The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress |
title_fullStr | The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress |
title_full_unstemmed | The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress |
title_short | The Role of the FOXO1/β(2)-AR/p-NF-κB p65 Pathway in the Development of Endometrial Stromal Cells in Pregnant Mice under Restraint Stress |
title_sort | role of the foxo1/β(2)-ar/p-nf-κb p65 pathway in the development of endometrial stromal cells in pregnant mice under restraint stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867244/ https://www.ncbi.nlm.nih.gov/pubmed/33540675 http://dx.doi.org/10.3390/ijms22031478 |
work_keys_str_mv | AT lujiayin theroleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT chenyaoxing theroleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT wangzixu theroleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT caojing theroleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT dongyulan theroleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT lujiayin roleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT chenyaoxing roleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT wangzixu roleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT caojing roleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress AT dongyulan roleofthefoxo1b2arpnfkbp65pathwayinthedevelopmentofendometrialstromalcellsinpregnantmiceunderrestraintstress |