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Exosomal microRNA-155 as a biomarker for hepatic fibrosis diagnosis and progression
BACKGROUND: Pathological examination of liver biopsies remains the gold standard for evaluating the stage of hepatic fibrosis, which are a number of disadvantages associated with biopsy. The aim of the present study was to investigate the potential of exosomal microRNA (miR)-155 as a non-invasive bi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867899/ https://www.ncbi.nlm.nih.gov/pubmed/33569439 http://dx.doi.org/10.21037/atm-20-7787 |
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author | Niu, Lian-Jie Zhang, Ya-Min Huang, Tao Sun, Xian-Fu Luo, Su-Xia |
author_facet | Niu, Lian-Jie Zhang, Ya-Min Huang, Tao Sun, Xian-Fu Luo, Su-Xia |
author_sort | Niu, Lian-Jie |
collection | PubMed |
description | BACKGROUND: Pathological examination of liver biopsies remains the gold standard for evaluating the stage of hepatic fibrosis, which are a number of disadvantages associated with biopsy. The aim of the present study was to investigate the potential of exosomal microRNA (miR)-155 as a non-invasive biomarker for the diagnosis and progression of hepatic fibrosis. METHODS: Exosomal miR-155 quantity was analyzed by sampling serum exosomes of patients with hepatic fibrosis and a hepatic fibrosis rat model. A total of 94 patients were divided into three groups based on Child-Pugh rating. Additionally, 30 patients with primary liver fibrosis who underwent liver transplantation were divided into the low miR-155 expression group and the high expression group; 56 rats were divided into 7 groups (n=8, 0, 2, 4, 6, 8, 10, and 12 weeks). Rats in every group were intravenously injected with CCl4 (3% vol/vol in olive oil; 0.3 mL/100 g body weight) twice weekly to produce different degrees of liver necrosis and liver fibrosis. RESULTS: Exosomal miR-155 was found to be closely associated with the progression of cirrhosis and clinical prognostic indicators of cirrhosis. Exosomal miR-155 gradually increased with the severity of hepatic necrosis and fibrosis. CONCLUSIONS: The findings of the present study indicate that exosomal miR-155 can act as a non-invasive biomarker for the diagnosis and progression of hepatic fibrosis. |
format | Online Article Text |
id | pubmed-7867899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-78678992021-02-09 Exosomal microRNA-155 as a biomarker for hepatic fibrosis diagnosis and progression Niu, Lian-Jie Zhang, Ya-Min Huang, Tao Sun, Xian-Fu Luo, Su-Xia Ann Transl Med Original Article BACKGROUND: Pathological examination of liver biopsies remains the gold standard for evaluating the stage of hepatic fibrosis, which are a number of disadvantages associated with biopsy. The aim of the present study was to investigate the potential of exosomal microRNA (miR)-155 as a non-invasive biomarker for the diagnosis and progression of hepatic fibrosis. METHODS: Exosomal miR-155 quantity was analyzed by sampling serum exosomes of patients with hepatic fibrosis and a hepatic fibrosis rat model. A total of 94 patients were divided into three groups based on Child-Pugh rating. Additionally, 30 patients with primary liver fibrosis who underwent liver transplantation were divided into the low miR-155 expression group and the high expression group; 56 rats were divided into 7 groups (n=8, 0, 2, 4, 6, 8, 10, and 12 weeks). Rats in every group were intravenously injected with CCl4 (3% vol/vol in olive oil; 0.3 mL/100 g body weight) twice weekly to produce different degrees of liver necrosis and liver fibrosis. RESULTS: Exosomal miR-155 was found to be closely associated with the progression of cirrhosis and clinical prognostic indicators of cirrhosis. Exosomal miR-155 gradually increased with the severity of hepatic necrosis and fibrosis. CONCLUSIONS: The findings of the present study indicate that exosomal miR-155 can act as a non-invasive biomarker for the diagnosis and progression of hepatic fibrosis. AME Publishing Company 2021-01 /pmc/articles/PMC7867899/ /pubmed/33569439 http://dx.doi.org/10.21037/atm-20-7787 Text en 2021 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Original Article Niu, Lian-Jie Zhang, Ya-Min Huang, Tao Sun, Xian-Fu Luo, Su-Xia Exosomal microRNA-155 as a biomarker for hepatic fibrosis diagnosis and progression |
title | Exosomal microRNA-155 as a biomarker for hepatic fibrosis diagnosis and progression |
title_full | Exosomal microRNA-155 as a biomarker for hepatic fibrosis diagnosis and progression |
title_fullStr | Exosomal microRNA-155 as a biomarker for hepatic fibrosis diagnosis and progression |
title_full_unstemmed | Exosomal microRNA-155 as a biomarker for hepatic fibrosis diagnosis and progression |
title_short | Exosomal microRNA-155 as a biomarker for hepatic fibrosis diagnosis and progression |
title_sort | exosomal microrna-155 as a biomarker for hepatic fibrosis diagnosis and progression |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7867899/ https://www.ncbi.nlm.nih.gov/pubmed/33569439 http://dx.doi.org/10.21037/atm-20-7787 |
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