Cargando…

The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue

Physical exercise is characterized by an increase in physical and metabolic demand in face of physical stress. It is reported that a single exercise session induces physiological responses through redox signaling to increase cellular function and energy support in diverse organs. However, little is...

Descripción completa

Detalles Bibliográficos
Autores principales: Matta, Leonardo, Fonseca, Túlio S., Faria, Caroline C., Lima-Junior, Niedson Correia, De Oliveira, Dahienne F., Maciel, Leonardo, Boa, Luiz F., Pierucci, Ana Paola T. R., Ferreira, Andrea C. F., Nascimento, José H. M., Carvalho, Denise P., Fortunato, Rodrigo S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868166/
https://www.ncbi.nlm.nih.gov/pubmed/33603946
http://dx.doi.org/10.1155/2021/4593496
_version_ 1783648411772256256
author Matta, Leonardo
Fonseca, Túlio S.
Faria, Caroline C.
Lima-Junior, Niedson Correia
De Oliveira, Dahienne F.
Maciel, Leonardo
Boa, Luiz F.
Pierucci, Ana Paola T. R.
Ferreira, Andrea C. F.
Nascimento, José H. M.
Carvalho, Denise P.
Fortunato, Rodrigo S.
author_facet Matta, Leonardo
Fonseca, Túlio S.
Faria, Caroline C.
Lima-Junior, Niedson Correia
De Oliveira, Dahienne F.
Maciel, Leonardo
Boa, Luiz F.
Pierucci, Ana Paola T. R.
Ferreira, Andrea C. F.
Nascimento, José H. M.
Carvalho, Denise P.
Fortunato, Rodrigo S.
author_sort Matta, Leonardo
collection PubMed
description Physical exercise is characterized by an increase in physical and metabolic demand in face of physical stress. It is reported that a single exercise session induces physiological responses through redox signaling to increase cellular function and energy support in diverse organs. However, little is known about the effect of a single bout of exercise on the redox homeostasis and cytoprotective gene expression of white adipose tissue (WAT). Thus, we aimed at evaluating the effects of acute aerobic exercise on WAT redox homeostasis, mitochondrial metabolism, and cytoprotective genic response. Male Wistar rats were submitted to a single moderate-high running session (treadmill) and were divided into five groups: control (CTRL, without exercise), and euthanized immediately (0 h), 30 min, 1 hour, or 2 hours after the end of the exercise session. NADPH oxidase activity was higher in 0 h and 30 min groups when compared to CTRL group. Extramitochondrial ROS production was higher in 0 h group in comparison to CTRL and 2 h groups. Mitochondrial respiration in phosphorylative state increased in 0 h group when compared to CTRL, 30 min, 1, and 2 h groups. On the other hand, mitochondrial ATP production was lower in 0 h in comparison to 30 min group, increasing in 1 and 2 h groups when compared to CTRL and 0 h groups. CAT activity was lower in all exercised groups when compared to CTRL. Regarding oxidative stress biomarkers, we observed a decrease in reduced thiol content in 0 h group compared to CTRL and 2 h groups, and higher levels of protein carbonylation in 0 and 30 min groups in comparison to the other groups. The levels returned to basal condition in 2 h group. Furthermore, aerobic exercise increased NRF2, GPX2, HMOX1, SOD1, and CAT mRNA levels. Taken together, our results suggest that one session of aerobic exercise can induce a transient prooxidative state in WAT, followed by an increase in antioxidant and cytoprotective gene expression.
format Online
Article
Text
id pubmed-7868166
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-78681662021-02-17 The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue Matta, Leonardo Fonseca, Túlio S. Faria, Caroline C. Lima-Junior, Niedson Correia De Oliveira, Dahienne F. Maciel, Leonardo Boa, Luiz F. Pierucci, Ana Paola T. R. Ferreira, Andrea C. F. Nascimento, José H. M. Carvalho, Denise P. Fortunato, Rodrigo S. Oxid Med Cell Longev Research Article Physical exercise is characterized by an increase in physical and metabolic demand in face of physical stress. It is reported that a single exercise session induces physiological responses through redox signaling to increase cellular function and energy support in diverse organs. However, little is known about the effect of a single bout of exercise on the redox homeostasis and cytoprotective gene expression of white adipose tissue (WAT). Thus, we aimed at evaluating the effects of acute aerobic exercise on WAT redox homeostasis, mitochondrial metabolism, and cytoprotective genic response. Male Wistar rats were submitted to a single moderate-high running session (treadmill) and were divided into five groups: control (CTRL, without exercise), and euthanized immediately (0 h), 30 min, 1 hour, or 2 hours after the end of the exercise session. NADPH oxidase activity was higher in 0 h and 30 min groups when compared to CTRL group. Extramitochondrial ROS production was higher in 0 h group in comparison to CTRL and 2 h groups. Mitochondrial respiration in phosphorylative state increased in 0 h group when compared to CTRL, 30 min, 1, and 2 h groups. On the other hand, mitochondrial ATP production was lower in 0 h in comparison to 30 min group, increasing in 1 and 2 h groups when compared to CTRL and 0 h groups. CAT activity was lower in all exercised groups when compared to CTRL. Regarding oxidative stress biomarkers, we observed a decrease in reduced thiol content in 0 h group compared to CTRL and 2 h groups, and higher levels of protein carbonylation in 0 and 30 min groups in comparison to the other groups. The levels returned to basal condition in 2 h group. Furthermore, aerobic exercise increased NRF2, GPX2, HMOX1, SOD1, and CAT mRNA levels. Taken together, our results suggest that one session of aerobic exercise can induce a transient prooxidative state in WAT, followed by an increase in antioxidant and cytoprotective gene expression. Hindawi 2021-01-31 /pmc/articles/PMC7868166/ /pubmed/33603946 http://dx.doi.org/10.1155/2021/4593496 Text en Copyright © 2021 Leonardo Matta et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Matta, Leonardo
Fonseca, Túlio S.
Faria, Caroline C.
Lima-Junior, Niedson Correia
De Oliveira, Dahienne F.
Maciel, Leonardo
Boa, Luiz F.
Pierucci, Ana Paola T. R.
Ferreira, Andrea C. F.
Nascimento, José H. M.
Carvalho, Denise P.
Fortunato, Rodrigo S.
The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue
title The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue
title_full The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue
title_fullStr The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue
title_full_unstemmed The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue
title_short The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue
title_sort effect of acute aerobic exercise on redox homeostasis and mitochondrial function of rat white adipose tissue
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868166/
https://www.ncbi.nlm.nih.gov/pubmed/33603946
http://dx.doi.org/10.1155/2021/4593496
work_keys_str_mv AT mattaleonardo theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT fonsecatulios theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT fariacarolinec theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT limajuniorniedsoncorreia theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT deoliveiradahiennef theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT macielleonardo theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT boaluizf theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT pieruccianapaolatr theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT ferreiraandreacf theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT nascimentojosehm theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT carvalhodenisep theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT fortunatorodrigos theeffectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT mattaleonardo effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT fonsecatulios effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT fariacarolinec effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT limajuniorniedsoncorreia effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT deoliveiradahiennef effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT macielleonardo effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT boaluizf effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT pieruccianapaolatr effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT ferreiraandreacf effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT nascimentojosehm effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT carvalhodenisep effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue
AT fortunatorodrigos effectofacuteaerobicexerciseonredoxhomeostasisandmitochondrialfunctionofratwhiteadiposetissue