Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes

The analysis of phagolysosomes within professional phagocytic cells is facilitated by their isolation. Here, we optimized a protocol for the isolation of intact phagolysosomes from macrophages infected with the spores of Aspergillus fumigatus. Purified phagolysosomes allow improved immunostaining, e...

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Detalles Bibliográficos
Autores principales: Goldmann, Marie, Schmidt, Franziska, Kyrmizi, Irene, Chamilos, Georgios, Brakhage, Axel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868627/
https://www.ncbi.nlm.nih.gov/pubmed/33598659
http://dx.doi.org/10.1016/j.xpro.2021.100328
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author Goldmann, Marie
Schmidt, Franziska
Kyrmizi, Irene
Chamilos, Georgios
Brakhage, Axel A.
author_facet Goldmann, Marie
Schmidt, Franziska
Kyrmizi, Irene
Chamilos, Georgios
Brakhage, Axel A.
author_sort Goldmann, Marie
collection PubMed
description The analysis of phagolysosomes within professional phagocytic cells is facilitated by their isolation. Here, we optimized a protocol for the isolation of intact phagolysosomes from macrophages infected with the spores of Aspergillus fumigatus. Purified phagolysosomes allow improved immunostaining, e.g., of phagolysosomal membrane proteins, or proteome analysis. For complete details on the use and execution of this protocol, please refer to Schmidt et al. (2020).
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spelling pubmed-78686272021-02-16 Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes Goldmann, Marie Schmidt, Franziska Kyrmizi, Irene Chamilos, Georgios Brakhage, Axel A. STAR Protoc Protocol The analysis of phagolysosomes within professional phagocytic cells is facilitated by their isolation. Here, we optimized a protocol for the isolation of intact phagolysosomes from macrophages infected with the spores of Aspergillus fumigatus. Purified phagolysosomes allow improved immunostaining, e.g., of phagolysosomal membrane proteins, or proteome analysis. For complete details on the use and execution of this protocol, please refer to Schmidt et al. (2020). Elsevier 2021-02-05 /pmc/articles/PMC7868627/ /pubmed/33598659 http://dx.doi.org/10.1016/j.xpro.2021.100328 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Goldmann, Marie
Schmidt, Franziska
Kyrmizi, Irene
Chamilos, Georgios
Brakhage, Axel A.
Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
title Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
title_full Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
title_fullStr Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
title_full_unstemmed Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
title_short Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
title_sort isolation and immunofluorescence staining of aspergillus fumigatus conidia-containing phagolysosomes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868627/
https://www.ncbi.nlm.nih.gov/pubmed/33598659
http://dx.doi.org/10.1016/j.xpro.2021.100328
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