Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes

The analysis of phagolysosomes within professional phagocytic cells is facilitated by their isolation. Here, we optimized a protocol for the isolation of intact phagolysosomes from macrophages infected with the spores of Aspergillus fumigatus. Purified phagolysosomes allow improved immunostaining, e...

Descripción completa

Detalles Bibliográficos
Autores principales: Goldmann, Marie, Schmidt, Franziska, Kyrmizi, Irene, Chamilos, Georgios, Brakhage, Axel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868627/
https://www.ncbi.nlm.nih.gov/pubmed/33598659
http://dx.doi.org/10.1016/j.xpro.2021.100328

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868627/
https://www.ncbi.nlm.nih.gov/pubmed/33598659
http://dx.doi.org/10.1016/j.xpro.2021.100328

Ejemplares similares