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Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
The analysis of phagolysosomes within professional phagocytic cells is facilitated by their isolation. Here, we optimized a protocol for the isolation of intact phagolysosomes from macrophages infected with the spores of Aspergillus fumigatus. Purified phagolysosomes allow improved immunostaining, e...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868627/ https://www.ncbi.nlm.nih.gov/pubmed/33598659 http://dx.doi.org/10.1016/j.xpro.2021.100328 |