Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

There is growing interest in medicine and sports in uncovering exercise modifiers that enhance or limit exercise capacity. Here, we detail a protocol for testing the daytime effect on running capacity in mice using a moderate intensity treadmill effort test. Instructions for dissecting soleus, gastr...

Descripción completa

Detalles Bibliográficos
Autores principales: Adamovich, Yaarit, Ezagouri, Saar, Dandavate, Vaishnavi, Asher, Gad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868630/
https://www.ncbi.nlm.nih.gov/pubmed/33598660
http://dx.doi.org/10.1016/j.xpro.2021.100331
_version_ 1783648489325985792
author Adamovich, Yaarit
Ezagouri, Saar
Dandavate, Vaishnavi
Asher, Gad
author_facet Adamovich, Yaarit
Ezagouri, Saar
Dandavate, Vaishnavi
Asher, Gad
author_sort Adamovich, Yaarit
collection PubMed
description There is growing interest in medicine and sports in uncovering exercise modifiers that enhance or limit exercise capacity. Here, we detail a protocol for testing the daytime effect on running capacity in mice using a moderate intensity treadmill effort test. Instructions for dissecting soleus, gastrocnemius plantaris, and quadriceps muscles for further analysis are provided as well. This experimental setup is optimized for addressing questions regarding the involvement of daytime and circadian clocks in regulating exercise capacity. For complete details on the use and execution of this protocol, please refer to Ezagouri et al. (2019).
format Online
Article
Text
id pubmed-7868630
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-78686302021-02-16 Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection Adamovich, Yaarit Ezagouri, Saar Dandavate, Vaishnavi Asher, Gad STAR Protoc Protocol There is growing interest in medicine and sports in uncovering exercise modifiers that enhance or limit exercise capacity. Here, we detail a protocol for testing the daytime effect on running capacity in mice using a moderate intensity treadmill effort test. Instructions for dissecting soleus, gastrocnemius plantaris, and quadriceps muscles for further analysis are provided as well. This experimental setup is optimized for addressing questions regarding the involvement of daytime and circadian clocks in regulating exercise capacity. For complete details on the use and execution of this protocol, please refer to Ezagouri et al. (2019). Elsevier 2021-02-05 /pmc/articles/PMC7868630/ /pubmed/33598660 http://dx.doi.org/10.1016/j.xpro.2021.100331 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Adamovich, Yaarit
Ezagouri, Saar
Dandavate, Vaishnavi
Asher, Gad
Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection
title Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection
title_full Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection
title_fullStr Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection
title_full_unstemmed Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection
title_short Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection
title_sort monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868630/
https://www.ncbi.nlm.nih.gov/pubmed/33598660
http://dx.doi.org/10.1016/j.xpro.2021.100331
work_keys_str_mv AT adamovichyaarit monitoringdaytimedifferencesinmoderateintensityexercisecapacityusingtreadmilltestandmuscledissection
AT ezagourisaar monitoringdaytimedifferencesinmoderateintensityexercisecapacityusingtreadmilltestandmuscledissection
AT dandavatevaishnavi monitoringdaytimedifferencesinmoderateintensityexercisecapacityusingtreadmilltestandmuscledissection
AT ashergad monitoringdaytimedifferencesinmoderateintensityexercisecapacityusingtreadmilltestandmuscledissection

Ejemplares similares