In vivo imaging of a PVD neuron in Caenorhabditis elegans

The nematode Caenorhabditis elegans nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A ser-2-promoter-driven GFP reporter line wyIs592[ser-2prom-3p::myr-GFP] provides a comprehensive visualization of PV...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xinjian, Li, Tingting, Hu, Jiawen, Feng, Zhigang, Zhong, Rui, Nie, Wang, Yang, Xiaoyan, Zou, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868634/
https://www.ncbi.nlm.nih.gov/pubmed/33598656
http://dx.doi.org/10.1016/j.xpro.2021.100309
_version_ 1783648490300112896
author Wang, Xinjian
Li, Tingting
Hu, Jiawen
Feng, Zhigang
Zhong, Rui
Nie, Wang
Yang, Xiaoyan
Zou, Yan
author_facet Wang, Xinjian
Li, Tingting
Hu, Jiawen
Feng, Zhigang
Zhong, Rui
Nie, Wang
Yang, Xiaoyan
Zou, Yan
author_sort Wang, Xinjian
collection PubMed
description The nematode Caenorhabditis elegans nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A ser-2-promoter-driven GFP reporter line wyIs592[ser-2prom-3p::myr-GFP] provides a comprehensive visualization of PVD anatomy. Here, we describe the detailed procedures for imaging a PVD neuron using wyIs592 at late L4 larval stage in vivo by confocal microscopy. This protocol can also be applied to imaging other cells in C. elegans. For complete details on the use and execution of this protocol, please refer to Feng et al. (2020).
format Online
Article
Text
id pubmed-7868634
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-78686342021-02-16 In vivo imaging of a PVD neuron in Caenorhabditis elegans Wang, Xinjian Li, Tingting Hu, Jiawen Feng, Zhigang Zhong, Rui Nie, Wang Yang, Xiaoyan Zou, Yan STAR Protoc Protocol The nematode Caenorhabditis elegans nociceptive PVD neurons have highly ordered dendritic branches, making this an ideal model to study the development and organization of dendrites. A ser-2-promoter-driven GFP reporter line wyIs592[ser-2prom-3p::myr-GFP] provides a comprehensive visualization of PVD anatomy. Here, we describe the detailed procedures for imaging a PVD neuron using wyIs592 at late L4 larval stage in vivo by confocal microscopy. This protocol can also be applied to imaging other cells in C. elegans. For complete details on the use and execution of this protocol, please refer to Feng et al. (2020). Elsevier 2021-02-04 /pmc/articles/PMC7868634/ /pubmed/33598656 http://dx.doi.org/10.1016/j.xpro.2021.100309 Text en © 2021. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wang, Xinjian
Li, Tingting
Hu, Jiawen
Feng, Zhigang
Zhong, Rui
Nie, Wang
Yang, Xiaoyan
Zou, Yan
In vivo imaging of a PVD neuron in Caenorhabditis elegans
title In vivo imaging of a PVD neuron in Caenorhabditis elegans
title_full In vivo imaging of a PVD neuron in Caenorhabditis elegans
title_fullStr In vivo imaging of a PVD neuron in Caenorhabditis elegans
title_full_unstemmed In vivo imaging of a PVD neuron in Caenorhabditis elegans
title_short In vivo imaging of a PVD neuron in Caenorhabditis elegans
title_sort in vivo imaging of a pvd neuron in caenorhabditis elegans
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868634/
https://www.ncbi.nlm.nih.gov/pubmed/33598656
http://dx.doi.org/10.1016/j.xpro.2021.100309
work_keys_str_mv AT wangxinjian invivoimagingofapvdneuronincaenorhabditiselegans
AT litingting invivoimagingofapvdneuronincaenorhabditiselegans
AT hujiawen invivoimagingofapvdneuronincaenorhabditiselegans
AT fengzhigang invivoimagingofapvdneuronincaenorhabditiselegans
AT zhongrui invivoimagingofapvdneuronincaenorhabditiselegans
AT niewang invivoimagingofapvdneuronincaenorhabditiselegans
AT yangxiaoyan invivoimagingofapvdneuronincaenorhabditiselegans
AT zouyan invivoimagingofapvdneuronincaenorhabditiselegans

Ejemplares similares