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miR-19a/b promote EMT and proliferation in glioma cells via SEPT7-AKT-NF-κB pathway

miR-19a/b belong to the miR-17-92 family. We have demonstrated previously that miR-19a/b are overexpressed in glioma and glioma cell lines. However, the role of miR-19a/b in glioma remains unclear. In the present study, we aim to identify the biological function and molecular mechanism of miR-19a/b...

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Autores principales: Wang, Weihan, Hao, Yubing, Zhang, Anling, Yang, Weidong, Wei, Wei, Wang, Guangxiu, Jia, Zhifan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868923/
https://www.ncbi.nlm.nih.gov/pubmed/33614912
http://dx.doi.org/10.1016/j.omto.2021.01.005
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author Wang, Weihan
Hao, Yubing
Zhang, Anling
Yang, Weidong
Wei, Wei
Wang, Guangxiu
Jia, Zhifan
author_facet Wang, Weihan
Hao, Yubing
Zhang, Anling
Yang, Weidong
Wei, Wei
Wang, Guangxiu
Jia, Zhifan
author_sort Wang, Weihan
collection PubMed
description miR-19a/b belong to the miR-17-92 family. We have demonstrated previously that miR-19a/b are overexpressed in glioma and glioma cell lines. However, the role of miR-19a/b in glioma remains unclear. In the present study, we aim to identify the biological function and molecular mechanism of miR-19a/b in glioma cell proliferation and epithelial-mesenchymal transition (EMT). Knocking down miR-19a/b in LN308 glioblastoma (GBM) cells with higher expression of miR-19a/b inhibits cell proliferation and invasion, induces apoptosis, and suppresses EMT by downregulating the expression of Akt, phosphorylated p-Akt, nuclear factor κB (NF-κB), Snail, N-cadherin, and Vimentin and upregulating E-cadherin in vitro and in vivo. Enhanced proliferation and EMT are also observed when miR-19a/b are transfected into SNB19 GBM cells, with lowered expression of miR-19a/b. miR-19a is more effective than miR-19b in the regulation of biological behavior of glioma cells. miR-19a/b modulate molecular events for the promotion of EMT via the Akt-NF-κB pathway. SEPT7 has been confirmed as the target gene of miR-19a/b. The effect of miR-19a/b on proliferation and EMT of glioma cells and the Akt-NF-κB pathway could be reversed by transfection with SEPT7. Our study strongly suggests that miR-19a/b play a significant role in glioma progression and EMT through regulating target gene-SEPT7 and the SEPT7-Akt-NF-κB pathway.
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spelling pubmed-78689232021-02-19 miR-19a/b promote EMT and proliferation in glioma cells via SEPT7-AKT-NF-κB pathway Wang, Weihan Hao, Yubing Zhang, Anling Yang, Weidong Wei, Wei Wang, Guangxiu Jia, Zhifan Mol Ther Oncolytics Original Article miR-19a/b belong to the miR-17-92 family. We have demonstrated previously that miR-19a/b are overexpressed in glioma and glioma cell lines. However, the role of miR-19a/b in glioma remains unclear. In the present study, we aim to identify the biological function and molecular mechanism of miR-19a/b in glioma cell proliferation and epithelial-mesenchymal transition (EMT). Knocking down miR-19a/b in LN308 glioblastoma (GBM) cells with higher expression of miR-19a/b inhibits cell proliferation and invasion, induces apoptosis, and suppresses EMT by downregulating the expression of Akt, phosphorylated p-Akt, nuclear factor κB (NF-κB), Snail, N-cadherin, and Vimentin and upregulating E-cadherin in vitro and in vivo. Enhanced proliferation and EMT are also observed when miR-19a/b are transfected into SNB19 GBM cells, with lowered expression of miR-19a/b. miR-19a is more effective than miR-19b in the regulation of biological behavior of glioma cells. miR-19a/b modulate molecular events for the promotion of EMT via the Akt-NF-κB pathway. SEPT7 has been confirmed as the target gene of miR-19a/b. The effect of miR-19a/b on proliferation and EMT of glioma cells and the Akt-NF-κB pathway could be reversed by transfection with SEPT7. Our study strongly suggests that miR-19a/b play a significant role in glioma progression and EMT through regulating target gene-SEPT7 and the SEPT7-Akt-NF-κB pathway. American Society of Gene & Cell Therapy 2021-01-16 /pmc/articles/PMC7868923/ /pubmed/33614912 http://dx.doi.org/10.1016/j.omto.2021.01.005 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Wang, Weihan
Hao, Yubing
Zhang, Anling
Yang, Weidong
Wei, Wei
Wang, Guangxiu
Jia, Zhifan
miR-19a/b promote EMT and proliferation in glioma cells via SEPT7-AKT-NF-κB pathway
title miR-19a/b promote EMT and proliferation in glioma cells via SEPT7-AKT-NF-κB pathway
title_full miR-19a/b promote EMT and proliferation in glioma cells via SEPT7-AKT-NF-κB pathway
title_fullStr miR-19a/b promote EMT and proliferation in glioma cells via SEPT7-AKT-NF-κB pathway
title_full_unstemmed miR-19a/b promote EMT and proliferation in glioma cells via SEPT7-AKT-NF-κB pathway
title_short miR-19a/b promote EMT and proliferation in glioma cells via SEPT7-AKT-NF-κB pathway
title_sort mir-19a/b promote emt and proliferation in glioma cells via sept7-akt-nf-κb pathway
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868923/
https://www.ncbi.nlm.nih.gov/pubmed/33614912
http://dx.doi.org/10.1016/j.omto.2021.01.005
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