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Development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through RNA interference

The rice black‐streaked dwarf virus (RBSDV) disease causes severe rice yield losses in Asia. RNA interference (RNAi) has been widely applied to develop antiviral varieties in plants. So far, only a few studies reported the application of RNAi in rice against RBSDV and most of them are lack of enough...

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Autores principales: Feng, Zhiming, Yuan, Meng, Zou, Jie, Wu, Lin‐Bo, Wei, Lang, Chen, Taiyu, Zhou, Nana, Xue, Wenxia, Zhang, Yafang, Chen, Zongxiang, Hu, Keming, Wang, Guo‐Liang, Liu, Wende, Pan, Xuebiao, Zuo, Shimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868976/
https://www.ncbi.nlm.nih.gov/pubmed/32741105
http://dx.doi.org/10.1111/pbi.13459
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author Feng, Zhiming
Yuan, Meng
Zou, Jie
Wu, Lin‐Bo
Wei, Lang
Chen, Taiyu
Zhou, Nana
Xue, Wenxia
Zhang, Yafang
Chen, Zongxiang
Hu, Keming
Wang, Guo‐Liang
Liu, Wende
Pan, Xuebiao
Zuo, Shimin
author_facet Feng, Zhiming
Yuan, Meng
Zou, Jie
Wu, Lin‐Bo
Wei, Lang
Chen, Taiyu
Zhou, Nana
Xue, Wenxia
Zhang, Yafang
Chen, Zongxiang
Hu, Keming
Wang, Guo‐Liang
Liu, Wende
Pan, Xuebiao
Zuo, Shimin
author_sort Feng, Zhiming
collection PubMed
description The rice black‐streaked dwarf virus (RBSDV) disease causes severe rice yield losses in Asia. RNA interference (RNAi) has been widely applied to develop antiviral varieties in plants. So far, only a few studies reported the application of RNAi in rice against RBSDV and most of them are lack of enough data to support its breeding potential, which limited the progress on developing RBSDV‐resistant variety. In this study, we generated three RNAi constructs to specifically target three RBSDV genes (S1, S2 and S6), respectively. We confirmed that RNAi targeting RBSDV S6 conferred rice with almost full immunity to RBSDV through phenotyping test in eight consecutive years in both artificial inoculation and field trials, while RNAi of S1 or S2 only leads to partially increased resistance. The S6RNAi was also found conferring strong resistance to southern rice black‐streaked dwarf virus (SRBSDV), a novel species closely related to RBSDV that outbroke recently in Southern China. In particular, no adverse effects on agronomical and developmental traits were found in S6RNAi transgenic lines. The marker‐free transgenic lines with S6RNAi, driven by either maize ubiquitin‐1 promoter or rice rbcS green tissue expression promoter, in elite rice background should have great potential in breeding of resistant varieties to both RBSDV and SRBSDV and provide a basis for further safety evaluation and commercial application.
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spelling pubmed-78689762021-02-17 Development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through RNA interference Feng, Zhiming Yuan, Meng Zou, Jie Wu, Lin‐Bo Wei, Lang Chen, Taiyu Zhou, Nana Xue, Wenxia Zhang, Yafang Chen, Zongxiang Hu, Keming Wang, Guo‐Liang Liu, Wende Pan, Xuebiao Zuo, Shimin Plant Biotechnol J Brief Communications The rice black‐streaked dwarf virus (RBSDV) disease causes severe rice yield losses in Asia. RNA interference (RNAi) has been widely applied to develop antiviral varieties in plants. So far, only a few studies reported the application of RNAi in rice against RBSDV and most of them are lack of enough data to support its breeding potential, which limited the progress on developing RBSDV‐resistant variety. In this study, we generated three RNAi constructs to specifically target three RBSDV genes (S1, S2 and S6), respectively. We confirmed that RNAi targeting RBSDV S6 conferred rice with almost full immunity to RBSDV through phenotyping test in eight consecutive years in both artificial inoculation and field trials, while RNAi of S1 or S2 only leads to partially increased resistance. The S6RNAi was also found conferring strong resistance to southern rice black‐streaked dwarf virus (SRBSDV), a novel species closely related to RBSDV that outbroke recently in Southern China. In particular, no adverse effects on agronomical and developmental traits were found in S6RNAi transgenic lines. The marker‐free transgenic lines with S6RNAi, driven by either maize ubiquitin‐1 promoter or rice rbcS green tissue expression promoter, in elite rice background should have great potential in breeding of resistant varieties to both RBSDV and SRBSDV and provide a basis for further safety evaluation and commercial application. John Wiley and Sons Inc. 2020-08-17 2021-02 /pmc/articles/PMC7868976/ /pubmed/32741105 http://dx.doi.org/10.1111/pbi.13459 Text en © 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communications
Feng, Zhiming
Yuan, Meng
Zou, Jie
Wu, Lin‐Bo
Wei, Lang
Chen, Taiyu
Zhou, Nana
Xue, Wenxia
Zhang, Yafang
Chen, Zongxiang
Hu, Keming
Wang, Guo‐Liang
Liu, Wende
Pan, Xuebiao
Zuo, Shimin
Development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through RNA interference
title Development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through RNA interference
title_full Development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through RNA interference
title_fullStr Development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through RNA interference
title_full_unstemmed Development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through RNA interference
title_short Development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through RNA interference
title_sort development of marker‐free rice with stable and high resistance to rice black‐streaked dwarf virus disease through rna interference
topic Brief Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868976/
https://www.ncbi.nlm.nih.gov/pubmed/32741105
http://dx.doi.org/10.1111/pbi.13459
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