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Helicobacter pylori Subdues Cytokine Signaling to Alter Mucosal Inflammation via Hypermethylation of Suppressor of Cytokine Signaling 1 Gene During Gastric Carcinogenesis

Helicobacter pylori infection has been associated with the onset of gastric mucosal inflammation and is known to perturb the balance between T-regulatory (Treg) and T-helper 17 (Th17) cells which causes a spurt of interleukin 17 (IL17) and transforming growth factor-β (TGF-β) from Th17 and Treg cell...

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Autores principales: Jan, Iqra, Rather, Rafiq A., Mushtaq, Ifra, Malik, Ajaz A., Besina, Syed, Baba, Abdul Basit, Farooq, Muzamil, Yousuf, Tahira, Rah, Bilal, Afroze, Dil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868987/
https://www.ncbi.nlm.nih.gov/pubmed/33569347
http://dx.doi.org/10.3389/fonc.2020.604747
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author Jan, Iqra
Rather, Rafiq A.
Mushtaq, Ifra
Malik, Ajaz A.
Besina, Syed
Baba, Abdul Basit
Farooq, Muzamil
Yousuf, Tahira
Rah, Bilal
Afroze, Dil
author_facet Jan, Iqra
Rather, Rafiq A.
Mushtaq, Ifra
Malik, Ajaz A.
Besina, Syed
Baba, Abdul Basit
Farooq, Muzamil
Yousuf, Tahira
Rah, Bilal
Afroze, Dil
author_sort Jan, Iqra
collection PubMed
description Helicobacter pylori infection has been associated with the onset of gastric mucosal inflammation and is known to perturb the balance between T-regulatory (Treg) and T-helper 17 (Th17) cells which causes a spurt of interleukin 17 (IL17) and transforming growth factor-β (TGF-β) from Th17 and Treg cells within the gastric milieu. IL17 instigates a surge of interleukin 6 (IL6) from T-helper 1 (Th1) and T-helper 2 (Th2) cells. Further, H. pylori infection is known to stimulate the atypical DNA methylation in gastric mucosa. However, the precise role of cytokine signaling in induction of epigenetic modifications during gastric carcinogenesis is vaguely understood. In this study, patient samples from were examined using real-time polymerase chain reaction (qPCR), PCR, methylation-specific (MS)-PCR, and enzyme-linked immunosorbent assays. We found that H. pylori infection augments the production of interleukin 10 (IL10), IL6, and TGF-β in the gastric milieu and systemic circulation. Together with the IL6/IL10 mediated hyperactivation of the JAK/STAT pathway, H. pylori infection causes the inactivation of suppressor of cytokine signaling 1 (SOCS1) gene through the hypermethylation of the promoter region. This study signifies that H. pylori-mediated epigenetic silencing of SOCS1 in concert with inflammatory cytokines miffs hyperactivation of the JAK/STAT cascade during gastric carcinogenesis.
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spelling pubmed-78689872021-02-09 Helicobacter pylori Subdues Cytokine Signaling to Alter Mucosal Inflammation via Hypermethylation of Suppressor of Cytokine Signaling 1 Gene During Gastric Carcinogenesis Jan, Iqra Rather, Rafiq A. Mushtaq, Ifra Malik, Ajaz A. Besina, Syed Baba, Abdul Basit Farooq, Muzamil Yousuf, Tahira Rah, Bilal Afroze, Dil Front Oncol Oncology Helicobacter pylori infection has been associated with the onset of gastric mucosal inflammation and is known to perturb the balance between T-regulatory (Treg) and T-helper 17 (Th17) cells which causes a spurt of interleukin 17 (IL17) and transforming growth factor-β (TGF-β) from Th17 and Treg cells within the gastric milieu. IL17 instigates a surge of interleukin 6 (IL6) from T-helper 1 (Th1) and T-helper 2 (Th2) cells. Further, H. pylori infection is known to stimulate the atypical DNA methylation in gastric mucosa. However, the precise role of cytokine signaling in induction of epigenetic modifications during gastric carcinogenesis is vaguely understood. In this study, patient samples from were examined using real-time polymerase chain reaction (qPCR), PCR, methylation-specific (MS)-PCR, and enzyme-linked immunosorbent assays. We found that H. pylori infection augments the production of interleukin 10 (IL10), IL6, and TGF-β in the gastric milieu and systemic circulation. Together with the IL6/IL10 mediated hyperactivation of the JAK/STAT pathway, H. pylori infection causes the inactivation of suppressor of cytokine signaling 1 (SOCS1) gene through the hypermethylation of the promoter region. This study signifies that H. pylori-mediated epigenetic silencing of SOCS1 in concert with inflammatory cytokines miffs hyperactivation of the JAK/STAT cascade during gastric carcinogenesis. Frontiers Media S.A. 2021-01-25 /pmc/articles/PMC7868987/ /pubmed/33569347 http://dx.doi.org/10.3389/fonc.2020.604747 Text en Copyright © 2021 Jan, Rather, Mushtaq, Malik, Besina, Baba, Farooq, Yousuf, Rah and Afroze http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Jan, Iqra
Rather, Rafiq A.
Mushtaq, Ifra
Malik, Ajaz A.
Besina, Syed
Baba, Abdul Basit
Farooq, Muzamil
Yousuf, Tahira
Rah, Bilal
Afroze, Dil
Helicobacter pylori Subdues Cytokine Signaling to Alter Mucosal Inflammation via Hypermethylation of Suppressor of Cytokine Signaling 1 Gene During Gastric Carcinogenesis
title Helicobacter pylori Subdues Cytokine Signaling to Alter Mucosal Inflammation via Hypermethylation of Suppressor of Cytokine Signaling 1 Gene During Gastric Carcinogenesis
title_full Helicobacter pylori Subdues Cytokine Signaling to Alter Mucosal Inflammation via Hypermethylation of Suppressor of Cytokine Signaling 1 Gene During Gastric Carcinogenesis
title_fullStr Helicobacter pylori Subdues Cytokine Signaling to Alter Mucosal Inflammation via Hypermethylation of Suppressor of Cytokine Signaling 1 Gene During Gastric Carcinogenesis
title_full_unstemmed Helicobacter pylori Subdues Cytokine Signaling to Alter Mucosal Inflammation via Hypermethylation of Suppressor of Cytokine Signaling 1 Gene During Gastric Carcinogenesis
title_short Helicobacter pylori Subdues Cytokine Signaling to Alter Mucosal Inflammation via Hypermethylation of Suppressor of Cytokine Signaling 1 Gene During Gastric Carcinogenesis
title_sort helicobacter pylori subdues cytokine signaling to alter mucosal inflammation via hypermethylation of suppressor of cytokine signaling 1 gene during gastric carcinogenesis
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868987/
https://www.ncbi.nlm.nih.gov/pubmed/33569347
http://dx.doi.org/10.3389/fonc.2020.604747
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