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In vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury

OBJECTIVES: To investigate the mechanisms underlying the protective effect of sufentanil against acute lung injury (ALI). MATERIAL AND METHODS: Rats were administered lipopolysaccharide (LPS) by endotracheal instillation to establish a model of ALI. LPS was used to stimulate BEAS-2B cells. The targe...

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Autores principales: Gao, Qi, Chang, Ningqing, Liu, Donglian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869068/
https://www.ncbi.nlm.nih.gov/pubmed/33535837
http://dx.doi.org/10.1177/0300060520986351
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author Gao, Qi
Chang, Ningqing
Liu, Donglian
author_facet Gao, Qi
Chang, Ningqing
Liu, Donglian
author_sort Gao, Qi
collection PubMed
description OBJECTIVES: To investigate the mechanisms underlying the protective effect of sufentanil against acute lung injury (ALI). MATERIAL AND METHODS: Rats were administered lipopolysaccharide (LPS) by endotracheal instillation to establish a model of ALI. LPS was used to stimulate BEAS-2B cells. The targets and promoter activities of IκB were assessed using a luciferase reporter assay. Apoptosis of BEAS-2B cells was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling. RESULTS: Sufentanil treatment markedly reduced pathological changes in lung tissue, pulmonary edema and secretion of inflammatory factors associated with ALI in vivo and in vitro. In addition, sufentanil suppressed apoptosis induced by LPS and activated NF-κB both in vivo and in vitro. Furthermore, upregulation of high mobility group box protein 1 (HMGB1) protein levels and downregulation of miR-129-5p levels were observed in vivo and in vitro following sufentanil treatment. miR-129-5p targeted the 3ʹ untranslated region and its inhibition decreased promoter activities of IκB-α. miR-129-5p inhibition significantly weakened the protective effect of sufentanil on LPS-treated BEAS-2B cells. CONCLUSION: Sufentanil regulated the miR-129-5p/HMGB1 axis to enhance IκB-α expression, suggesting that sufentanil represents a candidate drug for ALI protection and providing avenues for clinical treatment.
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spelling pubmed-78690682021-02-19 In vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury Gao, Qi Chang, Ningqing Liu, Donglian J Int Med Res Prospective Clinical Research Report OBJECTIVES: To investigate the mechanisms underlying the protective effect of sufentanil against acute lung injury (ALI). MATERIAL AND METHODS: Rats were administered lipopolysaccharide (LPS) by endotracheal instillation to establish a model of ALI. LPS was used to stimulate BEAS-2B cells. The targets and promoter activities of IκB were assessed using a luciferase reporter assay. Apoptosis of BEAS-2B cells was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling. RESULTS: Sufentanil treatment markedly reduced pathological changes in lung tissue, pulmonary edema and secretion of inflammatory factors associated with ALI in vivo and in vitro. In addition, sufentanil suppressed apoptosis induced by LPS and activated NF-κB both in vivo and in vitro. Furthermore, upregulation of high mobility group box protein 1 (HMGB1) protein levels and downregulation of miR-129-5p levels were observed in vivo and in vitro following sufentanil treatment. miR-129-5p targeted the 3ʹ untranslated region and its inhibition decreased promoter activities of IκB-α. miR-129-5p inhibition significantly weakened the protective effect of sufentanil on LPS-treated BEAS-2B cells. CONCLUSION: Sufentanil regulated the miR-129-5p/HMGB1 axis to enhance IκB-α expression, suggesting that sufentanil represents a candidate drug for ALI protection and providing avenues for clinical treatment. SAGE Publications 2021-02-04 /pmc/articles/PMC7869068/ /pubmed/33535837 http://dx.doi.org/10.1177/0300060520986351 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Prospective Clinical Research Report
Gao, Qi
Chang, Ningqing
Liu, Donglian
In vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury
title In vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury
title_full In vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury
title_fullStr In vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury
title_full_unstemmed In vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury
title_short In vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury
title_sort in vitro and in vivo assessment of the protective effect of sufentanil in acute lung injury
topic Prospective Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869068/
https://www.ncbi.nlm.nih.gov/pubmed/33535837
http://dx.doi.org/10.1177/0300060520986351
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