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Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress

In the mammalian skeletal system, osteogenesis and angiogenesis are closely linked by type H vessels during bone regeneration and repair. Our previous studies confirmed the promotion of these processes by copper-containing metal (CCM) in vitro and in vivo. However, whether and how the coupling of an...

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Autores principales: Xu, Daorong, Qian, Jikun, Guan, Xin, Ren, Ling, Yang, Kaifan, Huang, Xuan, Zhang, Shuyuan, Chai, Yu, Wu, Xiaohu, Wu, Hangtian, Zhang, Xianrong, Yang, Ke, Yu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869892/
https://www.ncbi.nlm.nih.gov/pubmed/33569374
http://dx.doi.org/10.3389/fbioe.2020.620629
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author Xu, Daorong
Qian, Jikun
Guan, Xin
Ren, Ling
Yang, Kaifan
Huang, Xuan
Zhang, Shuyuan
Chai, Yu
Wu, Xiaohu
Wu, Hangtian
Zhang, Xianrong
Yang, Ke
Yu, Bin
author_facet Xu, Daorong
Qian, Jikun
Guan, Xin
Ren, Ling
Yang, Kaifan
Huang, Xuan
Zhang, Shuyuan
Chai, Yu
Wu, Xiaohu
Wu, Hangtian
Zhang, Xianrong
Yang, Ke
Yu, Bin
author_sort Xu, Daorong
collection PubMed
description In the mammalian skeletal system, osteogenesis and angiogenesis are closely linked by type H vessels during bone regeneration and repair. Our previous studies confirmed the promotion of these processes by copper-containing metal (CCM) in vitro and in vivo. However, whether and how the coupling of angiogenesis and osteogenesis participates in the promotion of bone regeneration by CCM in vivo is unknown. In this study, M2a macrophages but not M2c macrophages were shown to be immunoregulated by CCM. A CCM, 316L−5Cu, was applied to drilling hole injuries of the tibia of C57/6 mice for comparison. We observed advanced formation of cortical bone and type H vessels beneath the new bone in the 316L−5Cu group 14 and 21 days postinjury. Moreover, the recruitment of CD206-positive M2a macrophages, which are regarded as the primary source of platelet-derived growth factor type BB (PDGF-BB), was significantly promoted at the injury site at days 14 and 21. Under the stimulation of CCM, mitochondria-derived reactive oxygen species were also found to be upregulated in CD206(hi) M2a macrophages in vitro, and this upregulation was correlated with the expression of PDGF-BB. In conclusion, our results indicate that CCM promotes the evolution of callus through the generation of type H vessels during the process of bone repair by upregulating the expression of PDGF-BB derived from M2a macrophages.
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spelling pubmed-78698922021-02-09 Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress Xu, Daorong Qian, Jikun Guan, Xin Ren, Ling Yang, Kaifan Huang, Xuan Zhang, Shuyuan Chai, Yu Wu, Xiaohu Wu, Hangtian Zhang, Xianrong Yang, Ke Yu, Bin Front Bioeng Biotechnol Bioengineering and Biotechnology In the mammalian skeletal system, osteogenesis and angiogenesis are closely linked by type H vessels during bone regeneration and repair. Our previous studies confirmed the promotion of these processes by copper-containing metal (CCM) in vitro and in vivo. However, whether and how the coupling of angiogenesis and osteogenesis participates in the promotion of bone regeneration by CCM in vivo is unknown. In this study, M2a macrophages but not M2c macrophages were shown to be immunoregulated by CCM. A CCM, 316L−5Cu, was applied to drilling hole injuries of the tibia of C57/6 mice for comparison. We observed advanced formation of cortical bone and type H vessels beneath the new bone in the 316L−5Cu group 14 and 21 days postinjury. Moreover, the recruitment of CD206-positive M2a macrophages, which are regarded as the primary source of platelet-derived growth factor type BB (PDGF-BB), was significantly promoted at the injury site at days 14 and 21. Under the stimulation of CCM, mitochondria-derived reactive oxygen species were also found to be upregulated in CD206(hi) M2a macrophages in vitro, and this upregulation was correlated with the expression of PDGF-BB. In conclusion, our results indicate that CCM promotes the evolution of callus through the generation of type H vessels during the process of bone repair by upregulating the expression of PDGF-BB derived from M2a macrophages. Frontiers Media S.A. 2021-01-14 /pmc/articles/PMC7869892/ /pubmed/33569374 http://dx.doi.org/10.3389/fbioe.2020.620629 Text en Copyright © 2021 Xu, Qian, Guan, Ren, Yang, Huang, Zhang, Chai, Wu, Wu, Zhang, Yang and Yu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Xu, Daorong
Qian, Jikun
Guan, Xin
Ren, Ling
Yang, Kaifan
Huang, Xuan
Zhang, Shuyuan
Chai, Yu
Wu, Xiaohu
Wu, Hangtian
Zhang, Xianrong
Yang, Ke
Yu, Bin
Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress
title Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress
title_full Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress
title_fullStr Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress
title_full_unstemmed Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress
title_short Copper-Containing Alloy as Immunoregulatory Material in Bone Regeneration via Mitochondrial Oxidative Stress
title_sort copper-containing alloy as immunoregulatory material in bone regeneration via mitochondrial oxidative stress
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869892/
https://www.ncbi.nlm.nih.gov/pubmed/33569374
http://dx.doi.org/10.3389/fbioe.2020.620629
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