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The NKG2D ligand ULBP4 is not expressed by human monocytes

The C-type lectin-like receptor NKG2D contributes to the immunosurveillance of virally infected and malignant cells by cytotoxic lymphocytes. A peculiar and puzzling feature of the NKG2D-based immunorecognition system is the high number of ligands for this single immunoreceptor. In humans, there are...

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Autores principales: Lazarova, Mariya, Kim, Younghoon, Steinle, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870063/
https://www.ncbi.nlm.nih.gov/pubmed/33556116
http://dx.doi.org/10.1371/journal.pone.0246726
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author Lazarova, Mariya
Kim, Younghoon
Steinle, Alexander
author_facet Lazarova, Mariya
Kim, Younghoon
Steinle, Alexander
author_sort Lazarova, Mariya
collection PubMed
description The C-type lectin-like receptor NKG2D contributes to the immunosurveillance of virally infected and malignant cells by cytotoxic lymphocytes. A peculiar and puzzling feature of the NKG2D-based immunorecognition system is the high number of ligands for this single immunoreceptor. In humans, there are a total of eight NKG2D ligands (NKG2DL) comprising two members of the MIC (MICA, MICB) and six members of the ULBP family of glycoproteins (ULBP1 to ULBP6). While MICA has been extensively studied with regard to its biochemistry, cellular expression and function, very little is known about the NKG2DL ULBP4. This is, at least in part, due to its rather restricted expression by very few cell lines and tissues. Recently, constitutive ULBP4 expression by human monocytes was reported, questioning the view of tissue-restricted ULBP4 expression. Here, we scrutinized ULBP4 expression by human peripheral blood mononuclear cells and monocytes by analyzing ULBP4 transcripts and ULBP4 surface expression. In contrast to MICA, there was no ULBP4 expression detectable, neither by freshly isolated monocytes nor by PAMP-activated monocytes. However, a commercial antibody erroneously indicated surface ULBP4 on monocytes due to a non-ULBP4-specific binding activity, emphasizing the critical importance of validated reagents for life sciences. Collectively, our data show that ULBP4 is not expressed by monocytes, and likely also not by other peripheral blood immune cells, and therefore exhibits an expression pattern rather distinct from other human NKG2DL.
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spelling pubmed-78700632021-02-11 The NKG2D ligand ULBP4 is not expressed by human monocytes Lazarova, Mariya Kim, Younghoon Steinle, Alexander PLoS One Research Article The C-type lectin-like receptor NKG2D contributes to the immunosurveillance of virally infected and malignant cells by cytotoxic lymphocytes. A peculiar and puzzling feature of the NKG2D-based immunorecognition system is the high number of ligands for this single immunoreceptor. In humans, there are a total of eight NKG2D ligands (NKG2DL) comprising two members of the MIC (MICA, MICB) and six members of the ULBP family of glycoproteins (ULBP1 to ULBP6). While MICA has been extensively studied with regard to its biochemistry, cellular expression and function, very little is known about the NKG2DL ULBP4. This is, at least in part, due to its rather restricted expression by very few cell lines and tissues. Recently, constitutive ULBP4 expression by human monocytes was reported, questioning the view of tissue-restricted ULBP4 expression. Here, we scrutinized ULBP4 expression by human peripheral blood mononuclear cells and monocytes by analyzing ULBP4 transcripts and ULBP4 surface expression. In contrast to MICA, there was no ULBP4 expression detectable, neither by freshly isolated monocytes nor by PAMP-activated monocytes. However, a commercial antibody erroneously indicated surface ULBP4 on monocytes due to a non-ULBP4-specific binding activity, emphasizing the critical importance of validated reagents for life sciences. Collectively, our data show that ULBP4 is not expressed by monocytes, and likely also not by other peripheral blood immune cells, and therefore exhibits an expression pattern rather distinct from other human NKG2DL. Public Library of Science 2021-02-08 /pmc/articles/PMC7870063/ /pubmed/33556116 http://dx.doi.org/10.1371/journal.pone.0246726 Text en © 2021 Lazarova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lazarova, Mariya
Kim, Younghoon
Steinle, Alexander
The NKG2D ligand ULBP4 is not expressed by human monocytes
title The NKG2D ligand ULBP4 is not expressed by human monocytes
title_full The NKG2D ligand ULBP4 is not expressed by human monocytes
title_fullStr The NKG2D ligand ULBP4 is not expressed by human monocytes
title_full_unstemmed The NKG2D ligand ULBP4 is not expressed by human monocytes
title_short The NKG2D ligand ULBP4 is not expressed by human monocytes
title_sort nkg2d ligand ulbp4 is not expressed by human monocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870063/
https://www.ncbi.nlm.nih.gov/pubmed/33556116
http://dx.doi.org/10.1371/journal.pone.0246726
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