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Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L.
Exposure to carbon tetrachloride (CCl(4)) induces acute and chronic liver injuries as well as oxidative stress in rats. The present study was designed to evaluate the in vivo toxicity of rosmarinic acid-rich extract from Ocimum basilicum (RAE). The acute and subchronic oral toxicity of RAE was evalu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870305/ https://www.ncbi.nlm.nih.gov/pubmed/33603821 http://dx.doi.org/10.1155/2021/6676998 |
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author | Touiss, Ilham Ouahhoud, Sabir Harnafi, Mohamed Khatib, Saloua Bekkouch, Oussama Amrani, Souliman Harnafi, Hicham |
author_facet | Touiss, Ilham Ouahhoud, Sabir Harnafi, Mohamed Khatib, Saloua Bekkouch, Oussama Amrani, Souliman Harnafi, Hicham |
author_sort | Touiss, Ilham |
collection | PubMed |
description | Exposure to carbon tetrachloride (CCl(4)) induces acute and chronic liver injuries as well as oxidative stress in rats. The present study was designed to evaluate the in vivo toxicity of rosmarinic acid-rich extract from Ocimum basilicum (RAE). The acute and subchronic oral toxicity of RAE was evaluated in Albinos mice. Hepatotoxicity was induced by the administration of CCl(4)-induced hepatic injury in rats. The hepatoprotective effect of RAE on aspartate aminotransferase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase, bilirubin, total protein, albumin, triglycerides, total cholesterol, low-density lipoprotein, high-density lipoprotein, plasmatic glucose, urea, creatinine, and malondialdehyde was determined in CCl(4)-intoxicated rat. The extract did not produce treatment-related signs of toxicity or mortality in any of the animals tested during acute as well as subchronic toxicity studies. The administration of CCl(4) resulted in marked increase in plasma hepatic enzymes (p < 0.001) and significant decrease of total protein (p < 0.001) and albumin (p < 0.001) when compared to normal. The RAE at 200 mg/kg body weight lowered significantly (p < 0.001) plasma enzyme activities of liver, which is designation of hepatoprotective action of extract. The phenolic extract exerts a significant increase in total protein (p < 0.001), and albumin (p < 0.001), accompanied with a marked reduction in the levels of malondialdehyde (p < 0.001), as compared to CCl(4)-treated group. Our study suggests that RAE may be used as a hepatoprotective agent against toxic effects caused by CCl(4) and other chemical agents in the liver. |
format | Online Article Text |
id | pubmed-7870305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-78703052021-02-17 Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L. Touiss, Ilham Ouahhoud, Sabir Harnafi, Mohamed Khatib, Saloua Bekkouch, Oussama Amrani, Souliman Harnafi, Hicham Evid Based Complement Alternat Med Research Article Exposure to carbon tetrachloride (CCl(4)) induces acute and chronic liver injuries as well as oxidative stress in rats. The present study was designed to evaluate the in vivo toxicity of rosmarinic acid-rich extract from Ocimum basilicum (RAE). The acute and subchronic oral toxicity of RAE was evaluated in Albinos mice. Hepatotoxicity was induced by the administration of CCl(4)-induced hepatic injury in rats. The hepatoprotective effect of RAE on aspartate aminotransferase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase, bilirubin, total protein, albumin, triglycerides, total cholesterol, low-density lipoprotein, high-density lipoprotein, plasmatic glucose, urea, creatinine, and malondialdehyde was determined in CCl(4)-intoxicated rat. The extract did not produce treatment-related signs of toxicity or mortality in any of the animals tested during acute as well as subchronic toxicity studies. The administration of CCl(4) resulted in marked increase in plasma hepatic enzymes (p < 0.001) and significant decrease of total protein (p < 0.001) and albumin (p < 0.001) when compared to normal. The RAE at 200 mg/kg body weight lowered significantly (p < 0.001) plasma enzyme activities of liver, which is designation of hepatoprotective action of extract. The phenolic extract exerts a significant increase in total protein (p < 0.001), and albumin (p < 0.001), accompanied with a marked reduction in the levels of malondialdehyde (p < 0.001), as compared to CCl(4)-treated group. Our study suggests that RAE may be used as a hepatoprotective agent against toxic effects caused by CCl(4) and other chemical agents in the liver. Hindawi 2021-01-31 /pmc/articles/PMC7870305/ /pubmed/33603821 http://dx.doi.org/10.1155/2021/6676998 Text en Copyright © 2021 Ilham Touiss et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Touiss, Ilham Ouahhoud, Sabir Harnafi, Mohamed Khatib, Saloua Bekkouch, Oussama Amrani, Souliman Harnafi, Hicham Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L. |
title | Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L. |
title_full | Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L. |
title_fullStr | Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L. |
title_full_unstemmed | Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L. |
title_short | Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum basilicum L. |
title_sort | toxicological evaluation and hepatoprotective efficacy of rosmarinic acid-rich extract from ocimum basilicum l. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870305/ https://www.ncbi.nlm.nih.gov/pubmed/33603821 http://dx.doi.org/10.1155/2021/6676998 |
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