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Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats

Proton pumps are membrane-bound enzymes important in generating gradients that help in maintaining cellular ion homeostasis, cell membrane potential, water, and solute transport across the cell surface. This study investigated the modulatory role of vitamin E on proton pump activity and reproductive...

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Autores principales: Olaniyan, Olugbemi T., Ojewale, Abdulfatai O., Eweoya, Olugbenga O., Adedoyin, Adetola A., Adesanya, Olamide A., Adeoye, Azeez O., Okeniran, Olatayo S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870308/
https://www.ncbi.nlm.nih.gov/pubmed/33604374
http://dx.doi.org/10.1155/2021/4615384
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author Olaniyan, Olugbemi T.
Ojewale, Abdulfatai O.
Eweoya, Olugbenga O.
Adedoyin, Adetola A.
Adesanya, Olamide A.
Adeoye, Azeez O.
Okeniran, Olatayo S.
author_facet Olaniyan, Olugbemi T.
Ojewale, Abdulfatai O.
Eweoya, Olugbenga O.
Adedoyin, Adetola A.
Adesanya, Olamide A.
Adeoye, Azeez O.
Okeniran, Olatayo S.
author_sort Olaniyan, Olugbemi T.
collection PubMed
description Proton pumps are membrane-bound enzymes important in generating gradients that help in maintaining cellular ion homeostasis, cell membrane potential, water, and solute transport across the cell surface. This study investigated the modulatory role of vitamin E on proton pump activity and reproductive parameters in cadmium-induced testicular damage. Twenty (20) male Wistar rats weighing between 180 and 200 g were sorted into 4 groups of five rats each. Group I served as the control and was given normal saline orally, Group II rats were treated with a single dose of 2 mg/kg BW cadmium chloride (CdCl(2)) intraperitoneally, Group III rats were given 100 mg/kg BW of vitamin E orally, and Group IV rats were given 100 mg/kg BW of vitamin E orally for 30 days prior to intraperitoneal administration of single dose of 2 mg/kg BW of cadmium chloride. The rats were anaesthetized with diethyl ether, and blood samples were obtained for sex hormonal analysis; caudal epididymis was dissected for sperm count, motility, and viability, and the testis were homogenized for lipid peroxidation and proton pump (Na(+)/K(+) ATPase, Ca(2+) ATPase, and Mg(2+) ATPase) activity. Proton pump activity was assayed spectrophotometrically using the Stewart method to determine the inorganic phosphate level. Histopathological changes of the testis were also studied. The group treated with CdCl(2) showed a significant (p < 0.05) decrease in proton pump activity, sperm count, and motility and a significant (p < 0.05) increase in malondialdehyde level when compared with the control group. The CdCl(2)-treated group also showed decrease reproductive organ weights and hormonal levels and cause necrosis of spermatogonia lining the seminiferous tubules. Rats treated with vitamin E orally for 30 days prior to CdCl(2) exposure showed improvement in proton pump activity, a significant (p < 0.05) increase in sperm parameters and luteinizing hormonal level, and a decrease in the lipid peroxidation level as compared with the CdCl(2) group. This study showed that vitamin E ameliorated the toxic effect of CdCl(2) on proton pump activity in the testes, hence improving testicular integrity, structures, and functions.
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spelling pubmed-78703082021-02-17 Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats Olaniyan, Olugbemi T. Ojewale, Abdulfatai O. Eweoya, Olugbenga O. Adedoyin, Adetola A. Adesanya, Olamide A. Adeoye, Azeez O. Okeniran, Olatayo S. Biomed Res Int Research Article Proton pumps are membrane-bound enzymes important in generating gradients that help in maintaining cellular ion homeostasis, cell membrane potential, water, and solute transport across the cell surface. This study investigated the modulatory role of vitamin E on proton pump activity and reproductive parameters in cadmium-induced testicular damage. Twenty (20) male Wistar rats weighing between 180 and 200 g were sorted into 4 groups of five rats each. Group I served as the control and was given normal saline orally, Group II rats were treated with a single dose of 2 mg/kg BW cadmium chloride (CdCl(2)) intraperitoneally, Group III rats were given 100 mg/kg BW of vitamin E orally, and Group IV rats were given 100 mg/kg BW of vitamin E orally for 30 days prior to intraperitoneal administration of single dose of 2 mg/kg BW of cadmium chloride. The rats were anaesthetized with diethyl ether, and blood samples were obtained for sex hormonal analysis; caudal epididymis was dissected for sperm count, motility, and viability, and the testis were homogenized for lipid peroxidation and proton pump (Na(+)/K(+) ATPase, Ca(2+) ATPase, and Mg(2+) ATPase) activity. Proton pump activity was assayed spectrophotometrically using the Stewart method to determine the inorganic phosphate level. Histopathological changes of the testis were also studied. The group treated with CdCl(2) showed a significant (p < 0.05) decrease in proton pump activity, sperm count, and motility and a significant (p < 0.05) increase in malondialdehyde level when compared with the control group. The CdCl(2)-treated group also showed decrease reproductive organ weights and hormonal levels and cause necrosis of spermatogonia lining the seminiferous tubules. Rats treated with vitamin E orally for 30 days prior to CdCl(2) exposure showed improvement in proton pump activity, a significant (p < 0.05) increase in sperm parameters and luteinizing hormonal level, and a decrease in the lipid peroxidation level as compared with the CdCl(2) group. This study showed that vitamin E ameliorated the toxic effect of CdCl(2) on proton pump activity in the testes, hence improving testicular integrity, structures, and functions. Hindawi 2021-02-01 /pmc/articles/PMC7870308/ /pubmed/33604374 http://dx.doi.org/10.1155/2021/4615384 Text en Copyright © 2021 Olugbemi T. Olaniyan et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Olaniyan, Olugbemi T.
Ojewale, Abdulfatai O.
Eweoya, Olugbenga O.
Adedoyin, Adetola A.
Adesanya, Olamide A.
Adeoye, Azeez O.
Okeniran, Olatayo S.
Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats
title Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats
title_full Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats
title_fullStr Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats
title_full_unstemmed Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats
title_short Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats
title_sort modulatory role of vitamin e on proton pump (atpase) activity of cadmium chloride-induced testicular damage in wistar rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870308/
https://www.ncbi.nlm.nih.gov/pubmed/33604374
http://dx.doi.org/10.1155/2021/4615384
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