Cargando…
Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of I...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870392/ https://www.ncbi.nlm.nih.gov/pubmed/33177289 http://dx.doi.org/10.1292/jvms.20-0423 |
_version_ | 1783648807216480256 |
---|---|
author | MITOMA, Shuya EL-KHAIAT, Heba M. UTO, Tomofumi SATO, Katsuaki SEKIGUCHI, Satoshi NORIMINE, Junzo |
author_facet | MITOMA, Shuya EL-KHAIAT, Heba M. UTO, Tomofumi SATO, Katsuaki SEKIGUCHI, Satoshi NORIMINE, Junzo |
author_sort | MITOMA, Shuya |
collection | PubMed |
description | Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of IL-2 is critical for deciding whether or not T cell responses against specific antigens result in protection. We have established a mammalian cell line (HEK-293) stably expressing bovine IL-2 (boIL-2) (designated as HEK-293/boIL-2), using the piggyBac transposon system. The concentration of recombinant bovine IL-2 (rboIL-2) in the culture supernatant of HEK-293/boIL-2 reached 100 ng/ml on day 7 and showed similar proliferative activity to recombinant human IL-2 (rhuIL-2) for bovine peripheral mononuclear blood cells. Although rhuIL-2 has been often used to activate bovine T cells, our results indicate that characteristics of the T cell activation through rboIL-2 and huIL-2 appear slightly but significantly different. Interestingly, the rboIL-2/anti-boIL-2 monoclonal antibody (C5) (rboIL-2/C5) complex strongly induced proliferation of bovine NKp46(+)cells, natural killer (NK) cells, in vitro. This indicates that the rboIL-2/C5 complex could function as an IL-2 agonist specifically to increase the NK cell population, which in turn could enhance the activity of NK cells leading to protective immunity. |
format | Online Article Text |
id | pubmed-7870392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78703922021-02-11 Characterization of bovine interleukin-2 stably expressed in HEK-293 cells MITOMA, Shuya EL-KHAIAT, Heba M. UTO, Tomofumi SATO, Katsuaki SEKIGUCHI, Satoshi NORIMINE, Junzo J Vet Med Sci Immunology Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of IL-2 is critical for deciding whether or not T cell responses against specific antigens result in protection. We have established a mammalian cell line (HEK-293) stably expressing bovine IL-2 (boIL-2) (designated as HEK-293/boIL-2), using the piggyBac transposon system. The concentration of recombinant bovine IL-2 (rboIL-2) in the culture supernatant of HEK-293/boIL-2 reached 100 ng/ml on day 7 and showed similar proliferative activity to recombinant human IL-2 (rhuIL-2) for bovine peripheral mononuclear blood cells. Although rhuIL-2 has been often used to activate bovine T cells, our results indicate that characteristics of the T cell activation through rboIL-2 and huIL-2 appear slightly but significantly different. Interestingly, the rboIL-2/anti-boIL-2 monoclonal antibody (C5) (rboIL-2/C5) complex strongly induced proliferation of bovine NKp46(+)cells, natural killer (NK) cells, in vitro. This indicates that the rboIL-2/C5 complex could function as an IL-2 agonist specifically to increase the NK cell population, which in turn could enhance the activity of NK cells leading to protective immunity. The Japanese Society of Veterinary Science 2020-11-11 2021-01 /pmc/articles/PMC7870392/ /pubmed/33177289 http://dx.doi.org/10.1292/jvms.20-0423 Text en ©2021 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Immunology MITOMA, Shuya EL-KHAIAT, Heba M. UTO, Tomofumi SATO, Katsuaki SEKIGUCHI, Satoshi NORIMINE, Junzo Characterization of bovine interleukin-2 stably expressed in HEK-293 cells |
title | Characterization of bovine interleukin-2 stably expressed in HEK-293 cells |
title_full | Characterization of bovine interleukin-2 stably expressed in HEK-293 cells |
title_fullStr | Characterization of bovine interleukin-2 stably expressed in HEK-293 cells |
title_full_unstemmed | Characterization of bovine interleukin-2 stably expressed in HEK-293 cells |
title_short | Characterization of bovine interleukin-2 stably expressed in HEK-293 cells |
title_sort | characterization of bovine interleukin-2 stably expressed in hek-293 cells |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870392/ https://www.ncbi.nlm.nih.gov/pubmed/33177289 http://dx.doi.org/10.1292/jvms.20-0423 |
work_keys_str_mv | AT mitomashuya characterizationofbovineinterleukin2stablyexpressedinhek293cells AT elkhaiathebam characterizationofbovineinterleukin2stablyexpressedinhek293cells AT utotomofumi characterizationofbovineinterleukin2stablyexpressedinhek293cells AT satokatsuaki characterizationofbovineinterleukin2stablyexpressedinhek293cells AT sekiguchisatoshi characterizationofbovineinterleukin2stablyexpressedinhek293cells AT noriminejunzo characterizationofbovineinterleukin2stablyexpressedinhek293cells |