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Characterization of bovine interleukin-2 stably expressed in HEK-293 cells

Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of I...

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Autores principales: MITOMA, Shuya, EL-KHAIAT, Heba M., UTO, Tomofumi, SATO, Katsuaki, SEKIGUCHI, Satoshi, NORIMINE, Junzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870392/
https://www.ncbi.nlm.nih.gov/pubmed/33177289
http://dx.doi.org/10.1292/jvms.20-0423
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author MITOMA, Shuya
EL-KHAIAT, Heba M.
UTO, Tomofumi
SATO, Katsuaki
SEKIGUCHI, Satoshi
NORIMINE, Junzo
author_facet MITOMA, Shuya
EL-KHAIAT, Heba M.
UTO, Tomofumi
SATO, Katsuaki
SEKIGUCHI, Satoshi
NORIMINE, Junzo
author_sort MITOMA, Shuya
collection PubMed
description Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of IL-2 is critical for deciding whether or not T cell responses against specific antigens result in protection. We have established a mammalian cell line (HEK-293) stably expressing bovine IL-2 (boIL-2) (designated as HEK-293/boIL-2), using the piggyBac transposon system. The concentration of recombinant bovine IL-2 (rboIL-2) in the culture supernatant of HEK-293/boIL-2 reached 100 ng/ml on day 7 and showed similar proliferative activity to recombinant human IL-2 (rhuIL-2) for bovine peripheral mononuclear blood cells. Although rhuIL-2 has been often used to activate bovine T cells, our results indicate that characteristics of the T cell activation through rboIL-2 and huIL-2 appear slightly but significantly different. Interestingly, the rboIL-2/anti-boIL-2 monoclonal antibody (C5) (rboIL-2/C5) complex strongly induced proliferation of bovine NKp46(+)cells, natural killer (NK) cells, in vitro. This indicates that the rboIL-2/C5 complex could function as an IL-2 agonist specifically to increase the NK cell population, which in turn could enhance the activity of NK cells leading to protective immunity.
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spelling pubmed-78703922021-02-11 Characterization of bovine interleukin-2 stably expressed in HEK-293 cells MITOMA, Shuya EL-KHAIAT, Heba M. UTO, Tomofumi SATO, Katsuaki SEKIGUCHI, Satoshi NORIMINE, Junzo J Vet Med Sci Immunology Interleukin 2 (IL-2) is a pleotropic cytokine and well-known as a T cell growth factor in immunology. It is now known to exert both immunostimulatory and immunosuppressive effects, optimizing immunological microenvironments for effector and regulatory T cell responses. The immunomodulatory role of IL-2 is critical for deciding whether or not T cell responses against specific antigens result in protection. We have established a mammalian cell line (HEK-293) stably expressing bovine IL-2 (boIL-2) (designated as HEK-293/boIL-2), using the piggyBac transposon system. The concentration of recombinant bovine IL-2 (rboIL-2) in the culture supernatant of HEK-293/boIL-2 reached 100 ng/ml on day 7 and showed similar proliferative activity to recombinant human IL-2 (rhuIL-2) for bovine peripheral mononuclear blood cells. Although rhuIL-2 has been often used to activate bovine T cells, our results indicate that characteristics of the T cell activation through rboIL-2 and huIL-2 appear slightly but significantly different. Interestingly, the rboIL-2/anti-boIL-2 monoclonal antibody (C5) (rboIL-2/C5) complex strongly induced proliferation of bovine NKp46(+)cells, natural killer (NK) cells, in vitro. This indicates that the rboIL-2/C5 complex could function as an IL-2 agonist specifically to increase the NK cell population, which in turn could enhance the activity of NK cells leading to protective immunity. The Japanese Society of Veterinary Science 2020-11-11 2021-01 /pmc/articles/PMC7870392/ /pubmed/33177289 http://dx.doi.org/10.1292/jvms.20-0423 Text en ©2021 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Immunology
MITOMA, Shuya
EL-KHAIAT, Heba M.
UTO, Tomofumi
SATO, Katsuaki
SEKIGUCHI, Satoshi
NORIMINE, Junzo
Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
title Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
title_full Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
title_fullStr Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
title_full_unstemmed Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
title_short Characterization of bovine interleukin-2 stably expressed in HEK-293 cells
title_sort characterization of bovine interleukin-2 stably expressed in hek-293 cells
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870392/
https://www.ncbi.nlm.nih.gov/pubmed/33177289
http://dx.doi.org/10.1292/jvms.20-0423
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