Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves

Bovine Mycoplasma arthritis (MA) is caused by Mycoplasma bovis and exhibits severe clinical symptoms. However, the pathophysiology of bovine MA is incompletely understood. In this study, we examined the cytokine mRNA expression of synovial fluid (SF) cells and cytokine concentrations in the SF of MA...

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Autores principales: NISHI, Koji, GONDAIRA, Satoshi, OKAMOTO, Mariko, MATSUDA, Kazuya, SATO, Ayano, KATO, Toshihide, SASAGAWA, Misawo, TANAKA, Takahiro, HIGUCHI, Hidetoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Internal Medicine
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870416/
https://www.ncbi.nlm.nih.gov/pubmed/33431726
http://dx.doi.org/10.1292/jvms.20-0491
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author NISHI, Koji
GONDAIRA, Satoshi
OKAMOTO, Mariko
MATSUDA, Kazuya
SATO, Ayano
KATO, Toshihide
SASAGAWA, Misawo
TANAKA, Takahiro
HIGUCHI, Hidetoshi
author_facet NISHI, Koji
GONDAIRA, Satoshi
OKAMOTO, Mariko
MATSUDA, Kazuya
SATO, Ayano
KATO, Toshihide
SASAGAWA, Misawo
TANAKA, Takahiro
HIGUCHI, Hidetoshi
author_sort NISHI, Koji
collection PubMed
description Bovine Mycoplasma arthritis (MA) is caused by Mycoplasma bovis and exhibits severe clinical symptoms. However, the pathophysiology of bovine MA is incompletely understood. In this study, we examined the cytokine mRNA expression of synovial fluid (SF) cells and cytokine concentrations in the SF of MA calves. The SF was isolated from five clinically healthy (control) and seven MA calves. mRNA and protein levels of interleukin (IL)-1β, IL-6, IL-8, IL-12, and IL-17 in the SF from MA calves were significantly higher than those from control calves. Our results indicate that SF cells produce inflammatory cytokines, which mainly contribute to the severe inflammatory response in the joints of the MA calves.
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spelling pubmed-78704162021-02-11 Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves NISHI, Koji GONDAIRA, Satoshi OKAMOTO, Mariko MATSUDA, Kazuya SATO, Ayano KATO, Toshihide SASAGAWA, Misawo TANAKA, Takahiro HIGUCHI, Hidetoshi J Vet Med Sci Internal Medicine Bovine Mycoplasma arthritis (MA) is caused by Mycoplasma bovis and exhibits severe clinical symptoms. However, the pathophysiology of bovine MA is incompletely understood. In this study, we examined the cytokine mRNA expression of synovial fluid (SF) cells and cytokine concentrations in the SF of MA calves. The SF was isolated from five clinically healthy (control) and seven MA calves. mRNA and protein levels of interleukin (IL)-1β, IL-6, IL-8, IL-12, and IL-17 in the SF from MA calves were significantly higher than those from control calves. Our results indicate that SF cells produce inflammatory cytokines, which mainly contribute to the severe inflammatory response in the joints of the MA calves. The Japanese Society of Veterinary Science 2021-01-08 2021-01 /pmc/articles/PMC7870416/ /pubmed/33431726 http://dx.doi.org/10.1292/jvms.20-0491 Text en ©2021 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Internal Medicine
NISHI, Koji
GONDAIRA, Satoshi
OKAMOTO, Mariko
MATSUDA, Kazuya
SATO, Ayano
KATO, Toshihide
SASAGAWA, Misawo
TANAKA, Takahiro
HIGUCHI, Hidetoshi
Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves
title Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves
title_full Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves
title_fullStr Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves
title_full_unstemmed Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves
title_short Inflammatory cytokine mRNA and protein levels in the synovial fluid of Mycoplasma arthritis calves
title_sort inflammatory cytokine mrna and protein levels in the synovial fluid of mycoplasma arthritis calves
topic Internal Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870416/
https://www.ncbi.nlm.nih.gov/pubmed/33431726
http://dx.doi.org/10.1292/jvms.20-0491
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