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Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer
Prostate cancer (PCa) is the most common malignant tumor in men, and its incidence increases with age. Serum prostate-specific antigen and tissue biopsy remain the standard for diagnosis of suspected PCa. However, these clinical indicators may lead to aggressive overtreatment in patients who have be...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870709/ https://www.ncbi.nlm.nih.gov/pubmed/33574834 http://dx.doi.org/10.3389/fgene.2021.636419 |
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author | Zhang, Lili Zhang, Wei Li, Hexin Tang, Xiaokun Xu, Siyuan Wu, Meng Wan, Li Su, Fei Zhang, Yaqun |
author_facet | Zhang, Lili Zhang, Wei Li, Hexin Tang, Xiaokun Xu, Siyuan Wu, Meng Wan, Li Su, Fei Zhang, Yaqun |
author_sort | Zhang, Lili |
collection | PubMed |
description | Prostate cancer (PCa) is the most common malignant tumor in men, and its incidence increases with age. Serum prostate-specific antigen and tissue biopsy remain the standard for diagnosis of suspected PCa. However, these clinical indicators may lead to aggressive overtreatment in patients who have been treated sufficiently with active surveillance. Circular RNAs (circRNAs) have been recently recognized as a new type of regulatory RNA that is not easily degraded by RNases and other exonucleases because of their covalent closed cyclic structure. Thus, we utilized high-throughput sequencing data and bioinformatics analysis to identify specifically expressed circRNAs in PCa and filtered out five specific circRNAs for further analysis—hsa_circ_0006410, hsa_circ_0003970, hsa_circ_0006754, hsa_circ_0005848, and a novel circRNA, hsa_circ_AKAP7. We constructed a circRNA-miRNA regulatory network and used miRNA and differentially expressed mRNA interactions to predict the function of the selected circRNAs. Furthermore, survival analysis of their cognate genes and PCR verification of these five circRNAs revealed that they are closely related to well-known PCa pathways such as the MAPK signaling pathway, P53 pathway, androgen receptor signaling pathway, cell cycle, hormone-mediated signaling pathway, and cellular lipid metabolic process. By understanding the related metabolism of circRNAs, these circRNAs could act as metabolic biomarkers, and monitoring their levels could help diagnose PCa. Meanwhile, the exact regulatory mechanism for AR-related regulation in PCa is still unclear. The circRNAs we found can provide new solutions for research in this field. |
format | Online Article Text |
id | pubmed-7870709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78707092021-02-10 Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer Zhang, Lili Zhang, Wei Li, Hexin Tang, Xiaokun Xu, Siyuan Wu, Meng Wan, Li Su, Fei Zhang, Yaqun Front Genet Genetics Prostate cancer (PCa) is the most common malignant tumor in men, and its incidence increases with age. Serum prostate-specific antigen and tissue biopsy remain the standard for diagnosis of suspected PCa. However, these clinical indicators may lead to aggressive overtreatment in patients who have been treated sufficiently with active surveillance. Circular RNAs (circRNAs) have been recently recognized as a new type of regulatory RNA that is not easily degraded by RNases and other exonucleases because of their covalent closed cyclic structure. Thus, we utilized high-throughput sequencing data and bioinformatics analysis to identify specifically expressed circRNAs in PCa and filtered out five specific circRNAs for further analysis—hsa_circ_0006410, hsa_circ_0003970, hsa_circ_0006754, hsa_circ_0005848, and a novel circRNA, hsa_circ_AKAP7. We constructed a circRNA-miRNA regulatory network and used miRNA and differentially expressed mRNA interactions to predict the function of the selected circRNAs. Furthermore, survival analysis of their cognate genes and PCR verification of these five circRNAs revealed that they are closely related to well-known PCa pathways such as the MAPK signaling pathway, P53 pathway, androgen receptor signaling pathway, cell cycle, hormone-mediated signaling pathway, and cellular lipid metabolic process. By understanding the related metabolism of circRNAs, these circRNAs could act as metabolic biomarkers, and monitoring their levels could help diagnose PCa. Meanwhile, the exact regulatory mechanism for AR-related regulation in PCa is still unclear. The circRNAs we found can provide new solutions for research in this field. Frontiers Media S.A. 2021-01-26 /pmc/articles/PMC7870709/ /pubmed/33574834 http://dx.doi.org/10.3389/fgene.2021.636419 Text en Copyright © 2021 Zhang, Zhang, Li, Tang, Xu, Wu, Wan, Su and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Zhang, Lili Zhang, Wei Li, Hexin Tang, Xiaokun Xu, Siyuan Wu, Meng Wan, Li Su, Fei Zhang, Yaqun Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer |
title | Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer |
title_full | Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer |
title_fullStr | Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer |
title_full_unstemmed | Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer |
title_short | Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer |
title_sort | five circular rnas in metabolism pathways related to prostate cancer |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870709/ https://www.ncbi.nlm.nih.gov/pubmed/33574834 http://dx.doi.org/10.3389/fgene.2021.636419 |
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