Cargando…

Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer

Prostate cancer (PCa) is the most common malignant tumor in men, and its incidence increases with age. Serum prostate-specific antigen and tissue biopsy remain the standard for diagnosis of suspected PCa. However, these clinical indicators may lead to aggressive overtreatment in patients who have be...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Lili, Zhang, Wei, Li, Hexin, Tang, Xiaokun, Xu, Siyuan, Wu, Meng, Wan, Li, Su, Fei, Zhang, Yaqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870709/
https://www.ncbi.nlm.nih.gov/pubmed/33574834
http://dx.doi.org/10.3389/fgene.2021.636419
_version_ 1783648863494602752
author Zhang, Lili
Zhang, Wei
Li, Hexin
Tang, Xiaokun
Xu, Siyuan
Wu, Meng
Wan, Li
Su, Fei
Zhang, Yaqun
author_facet Zhang, Lili
Zhang, Wei
Li, Hexin
Tang, Xiaokun
Xu, Siyuan
Wu, Meng
Wan, Li
Su, Fei
Zhang, Yaqun
author_sort Zhang, Lili
collection PubMed
description Prostate cancer (PCa) is the most common malignant tumor in men, and its incidence increases with age. Serum prostate-specific antigen and tissue biopsy remain the standard for diagnosis of suspected PCa. However, these clinical indicators may lead to aggressive overtreatment in patients who have been treated sufficiently with active surveillance. Circular RNAs (circRNAs) have been recently recognized as a new type of regulatory RNA that is not easily degraded by RNases and other exonucleases because of their covalent closed cyclic structure. Thus, we utilized high-throughput sequencing data and bioinformatics analysis to identify specifically expressed circRNAs in PCa and filtered out five specific circRNAs for further analysis—hsa_circ_0006410, hsa_circ_0003970, hsa_circ_0006754, hsa_circ_0005848, and a novel circRNA, hsa_circ_AKAP7. We constructed a circRNA-miRNA regulatory network and used miRNA and differentially expressed mRNA interactions to predict the function of the selected circRNAs. Furthermore, survival analysis of their cognate genes and PCR verification of these five circRNAs revealed that they are closely related to well-known PCa pathways such as the MAPK signaling pathway, P53 pathway, androgen receptor signaling pathway, cell cycle, hormone-mediated signaling pathway, and cellular lipid metabolic process. By understanding the related metabolism of circRNAs, these circRNAs could act as metabolic biomarkers, and monitoring their levels could help diagnose PCa. Meanwhile, the exact regulatory mechanism for AR-related regulation in PCa is still unclear. The circRNAs we found can provide new solutions for research in this field.
format Online
Article
Text
id pubmed-7870709
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78707092021-02-10 Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer Zhang, Lili Zhang, Wei Li, Hexin Tang, Xiaokun Xu, Siyuan Wu, Meng Wan, Li Su, Fei Zhang, Yaqun Front Genet Genetics Prostate cancer (PCa) is the most common malignant tumor in men, and its incidence increases with age. Serum prostate-specific antigen and tissue biopsy remain the standard for diagnosis of suspected PCa. However, these clinical indicators may lead to aggressive overtreatment in patients who have been treated sufficiently with active surveillance. Circular RNAs (circRNAs) have been recently recognized as a new type of regulatory RNA that is not easily degraded by RNases and other exonucleases because of their covalent closed cyclic structure. Thus, we utilized high-throughput sequencing data and bioinformatics analysis to identify specifically expressed circRNAs in PCa and filtered out five specific circRNAs for further analysis—hsa_circ_0006410, hsa_circ_0003970, hsa_circ_0006754, hsa_circ_0005848, and a novel circRNA, hsa_circ_AKAP7. We constructed a circRNA-miRNA regulatory network and used miRNA and differentially expressed mRNA interactions to predict the function of the selected circRNAs. Furthermore, survival analysis of their cognate genes and PCR verification of these five circRNAs revealed that they are closely related to well-known PCa pathways such as the MAPK signaling pathway, P53 pathway, androgen receptor signaling pathway, cell cycle, hormone-mediated signaling pathway, and cellular lipid metabolic process. By understanding the related metabolism of circRNAs, these circRNAs could act as metabolic biomarkers, and monitoring their levels could help diagnose PCa. Meanwhile, the exact regulatory mechanism for AR-related regulation in PCa is still unclear. The circRNAs we found can provide new solutions for research in this field. Frontiers Media S.A. 2021-01-26 /pmc/articles/PMC7870709/ /pubmed/33574834 http://dx.doi.org/10.3389/fgene.2021.636419 Text en Copyright © 2021 Zhang, Zhang, Li, Tang, Xu, Wu, Wan, Su and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhang, Lili
Zhang, Wei
Li, Hexin
Tang, Xiaokun
Xu, Siyuan
Wu, Meng
Wan, Li
Su, Fei
Zhang, Yaqun
Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer
title Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer
title_full Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer
title_fullStr Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer
title_full_unstemmed Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer
title_short Five Circular RNAs in Metabolism Pathways Related to Prostate Cancer
title_sort five circular rnas in metabolism pathways related to prostate cancer
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870709/
https://www.ncbi.nlm.nih.gov/pubmed/33574834
http://dx.doi.org/10.3389/fgene.2021.636419
work_keys_str_mv AT zhanglili fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer
AT zhangwei fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer
AT lihexin fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer
AT tangxiaokun fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer
AT xusiyuan fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer
AT wumeng fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer
AT wanli fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer
AT sufei fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer
AT zhangyaqun fivecircularrnasinmetabolismpathwaysrelatedtoprostatecancer