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The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis

The role of B cells in multiple sclerosis (MS) is increasingly recognized. B cells undergo compartmentalized redistribution in blood and cerebrospinal fluid (CSF) during active MS, whereby memory B cells accumulate in the CSF. While B-cell trafficking across the blood–brain barrier has been intensel...

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Autores principales: Haas, Jürgen, Rudolph, Henriette, Costa, Leonardo, Faller, Simon, Libicher, Saskia, Würthwein, Cornelia, Jarius, Sven, Ishikawa, Hiroshi, Stump-Guthier, Carolin, Tenenbaum, Tobias, Schwerk, Christian, Schroten, Horst, Wildemann, Brigitte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870993/
https://www.ncbi.nlm.nih.gov/pubmed/33574821
http://dx.doi.org/10.3389/fimmu.2020.618544
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author Haas, Jürgen
Rudolph, Henriette
Costa, Leonardo
Faller, Simon
Libicher, Saskia
Würthwein, Cornelia
Jarius, Sven
Ishikawa, Hiroshi
Stump-Guthier, Carolin
Tenenbaum, Tobias
Schwerk, Christian
Schroten, Horst
Wildemann, Brigitte
author_facet Haas, Jürgen
Rudolph, Henriette
Costa, Leonardo
Faller, Simon
Libicher, Saskia
Würthwein, Cornelia
Jarius, Sven
Ishikawa, Hiroshi
Stump-Guthier, Carolin
Tenenbaum, Tobias
Schwerk, Christian
Schroten, Horst
Wildemann, Brigitte
author_sort Haas, Jürgen
collection PubMed
description The role of B cells in multiple sclerosis (MS) is increasingly recognized. B cells undergo compartmentalized redistribution in blood and cerebrospinal fluid (CSF) during active MS, whereby memory B cells accumulate in the CSF. While B-cell trafficking across the blood–brain barrier has been intensely investigated, cellular diapedesis through the blood–CSF barrier (BCSFB) is incompletely understood. To investigate how B cells interact with the choroid plexus to transmigrate into the CSF we isolated circulating B cells from healthy donors (HC) and MS patients, utilized an inverted cell culture filter system of human choroid plexus papilloma (HIBCPP) cells to determine transmigration rates of B-cell subsets, immunofluorescence, and electron microscopy to analyze migration routes, and qRT-PCR to determine cytokines/chemokines mediating B-cell diapedesis. We also screened the transcriptome of intrathecal B cells from MS patients. We found, that spontaneous transmigration of HC- and MS-derived B cells was scant, yet increased significantly in response to B-cell specific chemokines CXCL-12/CXCL-13, was further boosted upon pre-activation and occurred via paracellular and transcellular pathways. Migrating cells exhibited upregulation of several genes involved in B-cell activation/migration and enhanced expression of chemokine receptors CXCR4/CXCR5, and were predominantly of isotype class switched memory phenotype. This antigen-experienced migratory subset displayed more pronounced chemotactic activities in MS than in HC and was retrieved in intrathecal B cells from patients with active MS. Trafficking of class-switched memory B cells was downscaled in a small cohort of natalizumab-exposed MS patients and the proportions of these phenotypes were reduced in peripheral blood yet were enriched intrathecally in patients who experienced recurrence of disease activity after withdrawal of natalizumab. Our findings highlight the relevance of the BCSFB as important gate for the entry of potentially harmful activated B cells into the CSF.
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spelling pubmed-78709932021-02-10 The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis Haas, Jürgen Rudolph, Henriette Costa, Leonardo Faller, Simon Libicher, Saskia Würthwein, Cornelia Jarius, Sven Ishikawa, Hiroshi Stump-Guthier, Carolin Tenenbaum, Tobias Schwerk, Christian Schroten, Horst Wildemann, Brigitte Front Immunol Immunology The role of B cells in multiple sclerosis (MS) is increasingly recognized. B cells undergo compartmentalized redistribution in blood and cerebrospinal fluid (CSF) during active MS, whereby memory B cells accumulate in the CSF. While B-cell trafficking across the blood–brain barrier has been intensely investigated, cellular diapedesis through the blood–CSF barrier (BCSFB) is incompletely understood. To investigate how B cells interact with the choroid plexus to transmigrate into the CSF we isolated circulating B cells from healthy donors (HC) and MS patients, utilized an inverted cell culture filter system of human choroid plexus papilloma (HIBCPP) cells to determine transmigration rates of B-cell subsets, immunofluorescence, and electron microscopy to analyze migration routes, and qRT-PCR to determine cytokines/chemokines mediating B-cell diapedesis. We also screened the transcriptome of intrathecal B cells from MS patients. We found, that spontaneous transmigration of HC- and MS-derived B cells was scant, yet increased significantly in response to B-cell specific chemokines CXCL-12/CXCL-13, was further boosted upon pre-activation and occurred via paracellular and transcellular pathways. Migrating cells exhibited upregulation of several genes involved in B-cell activation/migration and enhanced expression of chemokine receptors CXCR4/CXCR5, and were predominantly of isotype class switched memory phenotype. This antigen-experienced migratory subset displayed more pronounced chemotactic activities in MS than in HC and was retrieved in intrathecal B cells from patients with active MS. Trafficking of class-switched memory B cells was downscaled in a small cohort of natalizumab-exposed MS patients and the proportions of these phenotypes were reduced in peripheral blood yet were enriched intrathecally in patients who experienced recurrence of disease activity after withdrawal of natalizumab. Our findings highlight the relevance of the BCSFB as important gate for the entry of potentially harmful activated B cells into the CSF. Frontiers Media S.A. 2021-01-26 /pmc/articles/PMC7870993/ /pubmed/33574821 http://dx.doi.org/10.3389/fimmu.2020.618544 Text en Copyright © 2021 Haas, Rudolph, Costa, Faller, Libicher, Würthwein, Jarius, Ishikawa, Stump-Guthier, Tenenbaum, Schwerk, Schroten and Wildemann http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Haas, Jürgen
Rudolph, Henriette
Costa, Leonardo
Faller, Simon
Libicher, Saskia
Würthwein, Cornelia
Jarius, Sven
Ishikawa, Hiroshi
Stump-Guthier, Carolin
Tenenbaum, Tobias
Schwerk, Christian
Schroten, Horst
Wildemann, Brigitte
The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis
title The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis
title_full The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis
title_fullStr The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis
title_full_unstemmed The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis
title_short The Choroid Plexus Is Permissive for a Preactivated Antigen-Experienced Memory B-Cell Subset in Multiple Sclerosis
title_sort choroid plexus is permissive for a preactivated antigen-experienced memory b-cell subset in multiple sclerosis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870993/
https://www.ncbi.nlm.nih.gov/pubmed/33574821
http://dx.doi.org/10.3389/fimmu.2020.618544
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