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MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3

The functions of non-coding RNA, including microRNA (miRNA), have attracted considerable attention in the field of oncology, In this report, we examined the roles and molecular mechanisms of miR-128-3p, as related to the biological behaviors of malignant melanoma (MM). We found that miR-128-3p was e...

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Autores principales: Zhou, Xinxin, He, Jiayuan, Wang, Qingyuan, Ma, Teng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871904/
https://www.ncbi.nlm.nih.gov/pubmed/33575204
http://dx.doi.org/10.3389/fonc.2020.538894
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author Zhou, Xinxin
He, Jiayuan
Wang, Qingyuan
Ma, Teng
author_facet Zhou, Xinxin
He, Jiayuan
Wang, Qingyuan
Ma, Teng
author_sort Zhou, Xinxin
collection PubMed
description The functions of non-coding RNA, including microRNA (miRNA), have attracted considerable attention in the field of oncology, In this report, we examined the roles and molecular mechanisms of miR-128-3p, as related to the biological behaviors of malignant melanoma (MM). We found that miR-128-3p was expressed in low levels in these MM cells and may serve as a tumor suppressor by inhibiting proliferation, migration, and invasion, as well as inducing apoptosis in these MM cells. Moreover, neurotrophin receptor 3 (NTRK3), which serves as an oncogene that can enhance malignant behaviors of MM cells, was up-regulated in MM cells. Our current survey disclosed a complementary binding between miR-128-3p and the NTRK3 3′ untranslated regions (3′-UTR), while luciferase activities of NTRK3 3′-UTR were restrained by miR-128-3p in 293T cells. The effects of pre-miR-128-3p and sh-NTRK3 as well as anti-miR-128-3p and NTRK3(+) appeared to function synergistically in producing malignant progression. Moreover, there were possible to have counteracted effects for pre-miR-128-3p and NTRK3(+) in malignant progression. These findings established that miR-128-3p can function as a tumor suppressor by inhibiting carcinogenesis of the oncogene, NTRK3. Collectively, miR-128-3p and NTRK3 genes participate in modulating the malignant behavior of MM, and may represent new therapeutic targets for MM.
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spelling pubmed-78719042021-02-10 MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3 Zhou, Xinxin He, Jiayuan Wang, Qingyuan Ma, Teng Front Oncol Oncology The functions of non-coding RNA, including microRNA (miRNA), have attracted considerable attention in the field of oncology, In this report, we examined the roles and molecular mechanisms of miR-128-3p, as related to the biological behaviors of malignant melanoma (MM). We found that miR-128-3p was expressed in low levels in these MM cells and may serve as a tumor suppressor by inhibiting proliferation, migration, and invasion, as well as inducing apoptosis in these MM cells. Moreover, neurotrophin receptor 3 (NTRK3), which serves as an oncogene that can enhance malignant behaviors of MM cells, was up-regulated in MM cells. Our current survey disclosed a complementary binding between miR-128-3p and the NTRK3 3′ untranslated regions (3′-UTR), while luciferase activities of NTRK3 3′-UTR were restrained by miR-128-3p in 293T cells. The effects of pre-miR-128-3p and sh-NTRK3 as well as anti-miR-128-3p and NTRK3(+) appeared to function synergistically in producing malignant progression. Moreover, there were possible to have counteracted effects for pre-miR-128-3p and NTRK3(+) in malignant progression. These findings established that miR-128-3p can function as a tumor suppressor by inhibiting carcinogenesis of the oncogene, NTRK3. Collectively, miR-128-3p and NTRK3 genes participate in modulating the malignant behavior of MM, and may represent new therapeutic targets for MM. Frontiers Media S.A. 2021-01-26 /pmc/articles/PMC7871904/ /pubmed/33575204 http://dx.doi.org/10.3389/fonc.2020.538894 Text en Copyright © 2021 Zhou, He, Wang and Ma http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Zhou, Xinxin
He, Jiayuan
Wang, Qingyuan
Ma, Teng
MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3
title MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3
title_full MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3
title_fullStr MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3
title_full_unstemmed MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3
title_short MiRNA-128-3p Restrains Malignant Melanoma Cell Malignancy by Targeting NTRK3
title_sort mirna-128-3p restrains malignant melanoma cell malignancy by targeting ntrk3
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871904/
https://www.ncbi.nlm.nih.gov/pubmed/33575204
http://dx.doi.org/10.3389/fonc.2020.538894
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