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Analysis of the complete mitochondrial genomes of two forensically important blowfly species: Lucilia caesar and Lucilia illustris
Blowfly species of the family Calliphoridae can be used in forensic investigations to estimate the minimum post-mortem interval (PMI(min)). Lucilia caesar and Lucilia illustris (Diptera: Calliphoridae) are closely related and phenotypically similar, making reliable identification difficult. To ident...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871984/ https://www.ncbi.nlm.nih.gov/pubmed/33644383 http://dx.doi.org/10.1080/23802359.2018.1457991 |
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author | Schoofs, Kathleen R. Krzeminska Ahmadzai, Urszula Goodwin, William |
author_facet | Schoofs, Kathleen R. Krzeminska Ahmadzai, Urszula Goodwin, William |
author_sort | Schoofs, Kathleen R. |
collection | PubMed |
description | Blowfly species of the family Calliphoridae can be used in forensic investigations to estimate the minimum post-mortem interval (PMI(min)). Lucilia caesar and Lucilia illustris (Diptera: Calliphoridae) are closely related and phenotypically similar, making reliable identification difficult. To identify potential genetic markers to distinguish these species, five complete mitochondrial genomes were sequenced: three for L. caesar (KM657111–KM657113) and two for L. illustris (KM657109, KM657110). The ND6 gene contained the most species-specific SNPs (1.71%), followed by the ND5 gene (1.68%) and the COI gene (1.56%), identifying ND6 and ND5 as valuable loci for differentiating L. caesar and L. illustris specimens. |
format | Online Article Text |
id | pubmed-7871984 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-78719842021-02-26 Analysis of the complete mitochondrial genomes of two forensically important blowfly species: Lucilia caesar and Lucilia illustris Schoofs, Kathleen R. Krzeminska Ahmadzai, Urszula Goodwin, William Mitochondrial DNA B Resour Mito Communication Blowfly species of the family Calliphoridae can be used in forensic investigations to estimate the minimum post-mortem interval (PMI(min)). Lucilia caesar and Lucilia illustris (Diptera: Calliphoridae) are closely related and phenotypically similar, making reliable identification difficult. To identify potential genetic markers to distinguish these species, five complete mitochondrial genomes were sequenced: three for L. caesar (KM657111–KM657113) and two for L. illustris (KM657109, KM657110). The ND6 gene contained the most species-specific SNPs (1.71%), followed by the ND5 gene (1.68%) and the COI gene (1.56%), identifying ND6 and ND5 as valuable loci for differentiating L. caesar and L. illustris specimens. Taylor & Francis 2018-10-09 /pmc/articles/PMC7871984/ /pubmed/33644383 http://dx.doi.org/10.1080/23802359.2018.1457991 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Mito Communication Schoofs, Kathleen R. Krzeminska Ahmadzai, Urszula Goodwin, William Analysis of the complete mitochondrial genomes of two forensically important blowfly species: Lucilia caesar and Lucilia illustris |
title | Analysis of the complete mitochondrial genomes of two forensically important blowfly species: Lucilia caesar and Lucilia illustris |
title_full | Analysis of the complete mitochondrial genomes of two forensically important blowfly species: Lucilia caesar and Lucilia illustris |
title_fullStr | Analysis of the complete mitochondrial genomes of two forensically important blowfly species: Lucilia caesar and Lucilia illustris |
title_full_unstemmed | Analysis of the complete mitochondrial genomes of two forensically important blowfly species: Lucilia caesar and Lucilia illustris |
title_short | Analysis of the complete mitochondrial genomes of two forensically important blowfly species: Lucilia caesar and Lucilia illustris |
title_sort | analysis of the complete mitochondrial genomes of two forensically important blowfly species: lucilia caesar and lucilia illustris |
topic | Mito Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7871984/ https://www.ncbi.nlm.nih.gov/pubmed/33644383 http://dx.doi.org/10.1080/23802359.2018.1457991 |
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