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Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye

[Image: see text] Lyotropic myelin figures (MFs), i.e., long cylindrical structures formed by certain surfactants, owe their name to their resemblance to the biological membrane that covers nerve fibers. Herein, we used a strong bilayer-forming zwitterionic phospholipid stained by the Nile Red dye t...

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Autores principales: Benkowska-Biernacka, Dominika, Smalyukh, Ivan I., Matczyszyn, Katarzyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872420/
https://www.ncbi.nlm.nih.gov/pubmed/33347307
http://dx.doi.org/10.1021/acs.jpcb.0c08907
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author Benkowska-Biernacka, Dominika
Smalyukh, Ivan I.
Matczyszyn, Katarzyna
author_facet Benkowska-Biernacka, Dominika
Smalyukh, Ivan I.
Matczyszyn, Katarzyna
author_sort Benkowska-Biernacka, Dominika
collection PubMed
description [Image: see text] Lyotropic myelin figures (MFs), i.e., long cylindrical structures formed by certain surfactants, owe their name to their resemblance to the biological membrane that covers nerve fibers. Herein, we used a strong bilayer-forming zwitterionic phospholipid stained by the Nile Red dye to study lamellar mesophases. Polarized optical microscopy and fluorescence confocal microscopy allowed us to investigate the morphology of myelin structures and determine the orientational order of amphiphilic molecules. The cross-sectional views reveal significant differences in the configurations of MFs within the liquid crystalline cell, as well as the details of a spontaneous and stimulated formation of branched lipid tubes. Our results provide insights into small-scale morphology and out-of-equilibrium structural changes in the multilamellar structures.
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spelling pubmed-78724202021-02-10 Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye Benkowska-Biernacka, Dominika Smalyukh, Ivan I. Matczyszyn, Katarzyna J Phys Chem B [Image: see text] Lyotropic myelin figures (MFs), i.e., long cylindrical structures formed by certain surfactants, owe their name to their resemblance to the biological membrane that covers nerve fibers. Herein, we used a strong bilayer-forming zwitterionic phospholipid stained by the Nile Red dye to study lamellar mesophases. Polarized optical microscopy and fluorescence confocal microscopy allowed us to investigate the morphology of myelin structures and determine the orientational order of amphiphilic molecules. The cross-sectional views reveal significant differences in the configurations of MFs within the liquid crystalline cell, as well as the details of a spontaneous and stimulated formation of branched lipid tubes. Our results provide insights into small-scale morphology and out-of-equilibrium structural changes in the multilamellar structures. American Chemical Society 2020-12-21 2020-12-31 /pmc/articles/PMC7872420/ /pubmed/33347307 http://dx.doi.org/10.1021/acs.jpcb.0c08907 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Benkowska-Biernacka, Dominika
Smalyukh, Ivan I.
Matczyszyn, Katarzyna
Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye
title Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye
title_full Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye
title_fullStr Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye
title_full_unstemmed Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye
title_short Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye
title_sort morphology of lyotropic myelin figures stained with a fluorescent dye
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872420/
https://www.ncbi.nlm.nih.gov/pubmed/33347307
http://dx.doi.org/10.1021/acs.jpcb.0c08907
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