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Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye
[Image: see text] Lyotropic myelin figures (MFs), i.e., long cylindrical structures formed by certain surfactants, owe their name to their resemblance to the biological membrane that covers nerve fibers. Herein, we used a strong bilayer-forming zwitterionic phospholipid stained by the Nile Red dye t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872420/ https://www.ncbi.nlm.nih.gov/pubmed/33347307 http://dx.doi.org/10.1021/acs.jpcb.0c08907 |
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author | Benkowska-Biernacka, Dominika Smalyukh, Ivan I. Matczyszyn, Katarzyna |
author_facet | Benkowska-Biernacka, Dominika Smalyukh, Ivan I. Matczyszyn, Katarzyna |
author_sort | Benkowska-Biernacka, Dominika |
collection | PubMed |
description | [Image: see text] Lyotropic myelin figures (MFs), i.e., long cylindrical structures formed by certain surfactants, owe their name to their resemblance to the biological membrane that covers nerve fibers. Herein, we used a strong bilayer-forming zwitterionic phospholipid stained by the Nile Red dye to study lamellar mesophases. Polarized optical microscopy and fluorescence confocal microscopy allowed us to investigate the morphology of myelin structures and determine the orientational order of amphiphilic molecules. The cross-sectional views reveal significant differences in the configurations of MFs within the liquid crystalline cell, as well as the details of a spontaneous and stimulated formation of branched lipid tubes. Our results provide insights into small-scale morphology and out-of-equilibrium structural changes in the multilamellar structures. |
format | Online Article Text |
id | pubmed-7872420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-78724202021-02-10 Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye Benkowska-Biernacka, Dominika Smalyukh, Ivan I. Matczyszyn, Katarzyna J Phys Chem B [Image: see text] Lyotropic myelin figures (MFs), i.e., long cylindrical structures formed by certain surfactants, owe their name to their resemblance to the biological membrane that covers nerve fibers. Herein, we used a strong bilayer-forming zwitterionic phospholipid stained by the Nile Red dye to study lamellar mesophases. Polarized optical microscopy and fluorescence confocal microscopy allowed us to investigate the morphology of myelin structures and determine the orientational order of amphiphilic molecules. The cross-sectional views reveal significant differences in the configurations of MFs within the liquid crystalline cell, as well as the details of a spontaneous and stimulated formation of branched lipid tubes. Our results provide insights into small-scale morphology and out-of-equilibrium structural changes in the multilamellar structures. American Chemical Society 2020-12-21 2020-12-31 /pmc/articles/PMC7872420/ /pubmed/33347307 http://dx.doi.org/10.1021/acs.jpcb.0c08907 Text en © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Benkowska-Biernacka, Dominika Smalyukh, Ivan I. Matczyszyn, Katarzyna Morphology of Lyotropic Myelin Figures Stained with a Fluorescent Dye |
title | Morphology of Lyotropic Myelin Figures Stained with
a Fluorescent Dye |
title_full | Morphology of Lyotropic Myelin Figures Stained with
a Fluorescent Dye |
title_fullStr | Morphology of Lyotropic Myelin Figures Stained with
a Fluorescent Dye |
title_full_unstemmed | Morphology of Lyotropic Myelin Figures Stained with
a Fluorescent Dye |
title_short | Morphology of Lyotropic Myelin Figures Stained with
a Fluorescent Dye |
title_sort | morphology of lyotropic myelin figures stained with
a fluorescent dye |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872420/ https://www.ncbi.nlm.nih.gov/pubmed/33347307 http://dx.doi.org/10.1021/acs.jpcb.0c08907 |
work_keys_str_mv | AT benkowskabiernackadominika morphologyoflyotropicmyelinfiguresstainedwithafluorescentdye AT smalyukhivani morphologyoflyotropicmyelinfiguresstainedwithafluorescentdye AT matczyszynkatarzyna morphologyoflyotropicmyelinfiguresstainedwithafluorescentdye |