Cargando…
Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression
How moxibustion improves chronic heart failure is extremely complex and still unclear. This study aimed to explore whether moxibustion inhibits autophagy and reduces inflammation by regulating mTOR expression to induce myocardial protective effects and alleviate symptoms associated with chronic hear...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872756/ https://www.ncbi.nlm.nih.gov/pubmed/33603819 http://dx.doi.org/10.1155/2021/6635876 |
_version_ | 1783649246812045312 |
---|---|
author | Li, Qingling Wang, Wei Ma, Qiang Xia, Ran Gao, Bing Zhu, Guoqi Wang, Jing |
author_facet | Li, Qingling Wang, Wei Ma, Qiang Xia, Ran Gao, Bing Zhu, Guoqi Wang, Jing |
author_sort | Li, Qingling |
collection | PubMed |
description | How moxibustion improves chronic heart failure is extremely complex and still unclear. This study aimed to explore whether moxibustion inhibits autophagy and reduces inflammation by regulating mTOR expression to induce myocardial protective effects and alleviate symptoms associated with chronic heart failure. Echocardiography was used to detect cardiac function and cardiac structure of rats, including heart rate (HR), left atrium diameter (LA), left ventricular diameter (LV), left ventricular posterior wall (LVPW), interventricular septum (IVS), ejection fraction (EF), and fractional shortening (FS). BNP and NT-pro BNP levels were measured by enzyme-linked immunosorbent assay (ELISA). Autophagy-associated protein (ATG) genes and mTOR were detected by PCR. The expression of mTOR and phosphorylated-mTOR was detected through western blotting of proteins from myocardial tissue samples. The left ventricular inflammatory response was detected by immunohistochemistry and included ICAM-1, VCAM-1, MMP-2, and MMP-9 expression. The relationship between autophagy and inflammation was analyzed by correlation analysis. The results from echocardiography and ELISA showed that moxibustion could significantly improve heart function and structure. Western blot and PCR results showed that moxibustion treatment elevated mTOR expression. Further, moxibustion could inhibit autophagy and regulate the expression of key autophagy-related genes, including Vps34, ATG3, ATG5, ATG7, ATG12, and ATG13. By contrast, rapamycin could partially reduce the effects of moxibustion. Immunohistochemistry results indicated that moxibustion could reduce myocardial inflammation. Moreover, there was a positive correlation between autophagy and inflammation. Moxibustion can protect cardiac function in rats with heart failure, possibly inhibiting excessive autophagy of cardiomyocytes and reducing inflammatory reactions through the elevation of mTOR expression. |
format | Online Article Text |
id | pubmed-7872756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-78727562021-02-17 Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression Li, Qingling Wang, Wei Ma, Qiang Xia, Ran Gao, Bing Zhu, Guoqi Wang, Jing Evid Based Complement Alternat Med Research Article How moxibustion improves chronic heart failure is extremely complex and still unclear. This study aimed to explore whether moxibustion inhibits autophagy and reduces inflammation by regulating mTOR expression to induce myocardial protective effects and alleviate symptoms associated with chronic heart failure. Echocardiography was used to detect cardiac function and cardiac structure of rats, including heart rate (HR), left atrium diameter (LA), left ventricular diameter (LV), left ventricular posterior wall (LVPW), interventricular septum (IVS), ejection fraction (EF), and fractional shortening (FS). BNP and NT-pro BNP levels were measured by enzyme-linked immunosorbent assay (ELISA). Autophagy-associated protein (ATG) genes and mTOR were detected by PCR. The expression of mTOR and phosphorylated-mTOR was detected through western blotting of proteins from myocardial tissue samples. The left ventricular inflammatory response was detected by immunohistochemistry and included ICAM-1, VCAM-1, MMP-2, and MMP-9 expression. The relationship between autophagy and inflammation was analyzed by correlation analysis. The results from echocardiography and ELISA showed that moxibustion could significantly improve heart function and structure. Western blot and PCR results showed that moxibustion treatment elevated mTOR expression. Further, moxibustion could inhibit autophagy and regulate the expression of key autophagy-related genes, including Vps34, ATG3, ATG5, ATG7, ATG12, and ATG13. By contrast, rapamycin could partially reduce the effects of moxibustion. Immunohistochemistry results indicated that moxibustion could reduce myocardial inflammation. Moreover, there was a positive correlation between autophagy and inflammation. Moxibustion can protect cardiac function in rats with heart failure, possibly inhibiting excessive autophagy of cardiomyocytes and reducing inflammatory reactions through the elevation of mTOR expression. Hindawi 2021-02-02 /pmc/articles/PMC7872756/ /pubmed/33603819 http://dx.doi.org/10.1155/2021/6635876 Text en Copyright © 2021 Qingling Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Qingling Wang, Wei Ma, Qiang Xia, Ran Gao, Bing Zhu, Guoqi Wang, Jing Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression |
title | Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression |
title_full | Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression |
title_fullStr | Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression |
title_full_unstemmed | Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression |
title_short | Moxibustion Improves Chronic Heart Failure by Inhibiting Autophagy and Inflammation via Upregulation of mTOR Expression |
title_sort | moxibustion improves chronic heart failure by inhibiting autophagy and inflammation via upregulation of mtor expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872756/ https://www.ncbi.nlm.nih.gov/pubmed/33603819 http://dx.doi.org/10.1155/2021/6635876 |
work_keys_str_mv | AT liqingling moxibustionimproveschronicheartfailurebyinhibitingautophagyandinflammationviaupregulationofmtorexpression AT wangwei moxibustionimproveschronicheartfailurebyinhibitingautophagyandinflammationviaupregulationofmtorexpression AT maqiang moxibustionimproveschronicheartfailurebyinhibitingautophagyandinflammationviaupregulationofmtorexpression AT xiaran moxibustionimproveschronicheartfailurebyinhibitingautophagyandinflammationviaupregulationofmtorexpression AT gaobing moxibustionimproveschronicheartfailurebyinhibitingautophagyandinflammationviaupregulationofmtorexpression AT zhuguoqi moxibustionimproveschronicheartfailurebyinhibitingautophagyandinflammationviaupregulationofmtorexpression AT wangjing moxibustionimproveschronicheartfailurebyinhibitingautophagyandinflammationviaupregulationofmtorexpression |