Cargando…
Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease
BACKGROUND: It was indicated that nucleotide-binding oligomerisation domain‑like receptor protein 1 (NLRP1) inflammasome-mediated pyroptosis is involveg in the progression of Alzheimer’s disease (AD). This study was designed to explore the effect of Bushen Huoxue Acupuncture on cognitive defect and N...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872899/ https://www.ncbi.nlm.nih.gov/pubmed/33574670 http://dx.doi.org/10.2147/NDT.S279304 |
_version_ | 1783649279819120640 |
---|---|
author | Zhang, Ting Guan, Bin Tan, Sipin Zhu, Hong Ren, Dan Li, Ruomeng Xiao, Lan |
author_facet | Zhang, Ting Guan, Bin Tan, Sipin Zhu, Hong Ren, Dan Li, Ruomeng Xiao, Lan |
author_sort | Zhang, Ting |
collection | PubMed |
description | BACKGROUND: It was indicated that nucleotide-binding oligomerisation domain‑like receptor protein 1 (NLRP1) inflammasome-mediated pyroptosis is involveg in the progression of Alzheimer’s disease (AD). This study was designed to explore the effect of Bushen Huoxue Acupuncture on cognitive defect and NLRP1 inflammasome-mediated pyroptosis in AD mouse. METHODS: Senescence-accelerated mouse prone 8 (SAMP8) mice were used as a model of AD. Bushen Huoxue Acupuncture was performed in four acupoints: “Baihui acupoint” (GV20), “Shenshu acupoint” (BL23), “Xuehai acupoint” (SP10), and “Geshu acupoint” (BL17). Morris water maze test was performed to evaluate the cognitive function of the mouse. The levels of Aβ(1-40), Aβ(1-42), IL-1β, and IL-18 were examined by ELISA assay. Neuronal apoptosis and damage in hippocampal tissues were measured using TUNEL and Nissl staining, respectively. The expression of NLRP1, ASC, cleaved caspase-1, IL-1β, and IL-18 was examined using Western blot. RESULTS: Bushen Huoxue Acupuncture improved the learning and memory deficits of AD mouse. Meanwhile, Bushen Huoxue Acupuncture decreased the production of Aβ in hippocampal tissues of SAMP8 mice and attenuated the neuronal apoptosis and damage. Furthermore, Bushen Huoxue Acupuncture inhibited NLRP1 inflammasome activation in SAMP8 mice. CONCLUSION: Bushen Huoxue Acupuncture could notably attenuate the cognitive defect of mouse AD model and inhibit NLRP1 inflammasome-mediated pyroptosis. |
format | Online Article Text |
id | pubmed-7872899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-78728992021-02-10 Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease Zhang, Ting Guan, Bin Tan, Sipin Zhu, Hong Ren, Dan Li, Ruomeng Xiao, Lan Neuropsychiatr Dis Treat Original Research BACKGROUND: It was indicated that nucleotide-binding oligomerisation domain‑like receptor protein 1 (NLRP1) inflammasome-mediated pyroptosis is involveg in the progression of Alzheimer’s disease (AD). This study was designed to explore the effect of Bushen Huoxue Acupuncture on cognitive defect and NLRP1 inflammasome-mediated pyroptosis in AD mouse. METHODS: Senescence-accelerated mouse prone 8 (SAMP8) mice were used as a model of AD. Bushen Huoxue Acupuncture was performed in four acupoints: “Baihui acupoint” (GV20), “Shenshu acupoint” (BL23), “Xuehai acupoint” (SP10), and “Geshu acupoint” (BL17). Morris water maze test was performed to evaluate the cognitive function of the mouse. The levels of Aβ(1-40), Aβ(1-42), IL-1β, and IL-18 were examined by ELISA assay. Neuronal apoptosis and damage in hippocampal tissues were measured using TUNEL and Nissl staining, respectively. The expression of NLRP1, ASC, cleaved caspase-1, IL-1β, and IL-18 was examined using Western blot. RESULTS: Bushen Huoxue Acupuncture improved the learning and memory deficits of AD mouse. Meanwhile, Bushen Huoxue Acupuncture decreased the production of Aβ in hippocampal tissues of SAMP8 mice and attenuated the neuronal apoptosis and damage. Furthermore, Bushen Huoxue Acupuncture inhibited NLRP1 inflammasome activation in SAMP8 mice. CONCLUSION: Bushen Huoxue Acupuncture could notably attenuate the cognitive defect of mouse AD model and inhibit NLRP1 inflammasome-mediated pyroptosis. Dove 2021-02-05 /pmc/articles/PMC7872899/ /pubmed/33574670 http://dx.doi.org/10.2147/NDT.S279304 Text en © 2021 Zhang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Zhang, Ting Guan, Bin Tan, Sipin Zhu, Hong Ren, Dan Li, Ruomeng Xiao, Lan Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease |
title | Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease |
title_full | Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease |
title_fullStr | Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease |
title_full_unstemmed | Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease |
title_short | Bushen Huoxue Acupuncture Inhibits NLRP1 Inflammasome-Mediated Neuronal Pyroptosis in SAMP8 Mouse Model of Alzheimer’s Disease |
title_sort | bushen huoxue acupuncture inhibits nlrp1 inflammasome-mediated neuronal pyroptosis in samp8 mouse model of alzheimer’s disease |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872899/ https://www.ncbi.nlm.nih.gov/pubmed/33574670 http://dx.doi.org/10.2147/NDT.S279304 |
work_keys_str_mv | AT zhangting bushenhuoxueacupunctureinhibitsnlrp1inflammasomemediatedneuronalpyroptosisinsamp8mousemodelofalzheimersdisease AT guanbin bushenhuoxueacupunctureinhibitsnlrp1inflammasomemediatedneuronalpyroptosisinsamp8mousemodelofalzheimersdisease AT tansipin bushenhuoxueacupunctureinhibitsnlrp1inflammasomemediatedneuronalpyroptosisinsamp8mousemodelofalzheimersdisease AT zhuhong bushenhuoxueacupunctureinhibitsnlrp1inflammasomemediatedneuronalpyroptosisinsamp8mousemodelofalzheimersdisease AT rendan bushenhuoxueacupunctureinhibitsnlrp1inflammasomemediatedneuronalpyroptosisinsamp8mousemodelofalzheimersdisease AT liruomeng bushenhuoxueacupunctureinhibitsnlrp1inflammasomemediatedneuronalpyroptosisinsamp8mousemodelofalzheimersdisease AT xiaolan bushenhuoxueacupunctureinhibitsnlrp1inflammasomemediatedneuronalpyroptosisinsamp8mousemodelofalzheimersdisease |