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Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress
PURPOSE: Sporothrix schenckii is a thermally dimorphic fungus. In a saprotrophic environment or culturing at 25 °C, it grows as mycelia, whereas in host tissues or culturing at 37 °C, it undergoes dimorphic transition and division into pathogenic yeast cells. S. schenckii can cause serious dissemina...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873001/ https://www.ncbi.nlm.nih.gov/pubmed/32691258 http://dx.doi.org/10.1007/s10123-020-00136-y |
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author | He, Dan Zhang, Xiaowei Gao, Song You, Hailong Zhao, Yanbing Wang, Li |
author_facet | He, Dan Zhang, Xiaowei Gao, Song You, Hailong Zhao, Yanbing Wang, Li |
author_sort | He, Dan |
collection | PubMed |
description | PURPOSE: Sporothrix schenckii is a thermally dimorphic fungus. In a saprotrophic environment or culturing at 25 °C, it grows as mycelia, whereas in host tissues or culturing at 37 °C, it undergoes dimorphic transition and division into pathogenic yeast cells. S. schenckii can cause serious disseminated sporotrichosis in immunocompromised hosts and presents an emerging global health problem. The mycelium-to-yeast transition was a consequence of the adaptive process to different environment. Some studies showed that the transition was significantly related to the virulence and pathogenesis of dimorphic fungi. However the genetic mechanisms of this complicated biological process are poorly understood. METHOD: Our study presented a comparative transcriptomic analysis perspective on temperature stress in a visceral isolates of S. schenckii, obtaining more genetic information related to dimorphic transition. RESULTS: The 9.38 Gbp dataset was generated and assembled into 14,423 unigenes. Compared with gene and protein databases, 9561 unigenes were annotated. Comparative analysis identified 1259 genes expressed differentially in mycelium and yeast phase, and were categorized into a number of important biological processes, such as synthesis and metabolism, transmembrane transport, biocatalysis, oxidation reduction, and cellular signal transduction. CONCLUSIONS: The findings suggested that temperature-dependent transition was tightly associated with stress adaptation, growth and development, signal regulation, adhesion, and colonization, which was predicted to be related with virulence and pathogenesis. Collection of these data should offer fine-scale insights into the mechanisms of dimorphism and pathogenesis of S. schenckii, and meanwhile facilitate the evolutionary and function studies of other dimorphic fungi. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10123-020-00136-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7873001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-78730012021-02-22 Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress He, Dan Zhang, Xiaowei Gao, Song You, Hailong Zhao, Yanbing Wang, Li Int Microbiol Original Article PURPOSE: Sporothrix schenckii is a thermally dimorphic fungus. In a saprotrophic environment or culturing at 25 °C, it grows as mycelia, whereas in host tissues or culturing at 37 °C, it undergoes dimorphic transition and division into pathogenic yeast cells. S. schenckii can cause serious disseminated sporotrichosis in immunocompromised hosts and presents an emerging global health problem. The mycelium-to-yeast transition was a consequence of the adaptive process to different environment. Some studies showed that the transition was significantly related to the virulence and pathogenesis of dimorphic fungi. However the genetic mechanisms of this complicated biological process are poorly understood. METHOD: Our study presented a comparative transcriptomic analysis perspective on temperature stress in a visceral isolates of S. schenckii, obtaining more genetic information related to dimorphic transition. RESULTS: The 9.38 Gbp dataset was generated and assembled into 14,423 unigenes. Compared with gene and protein databases, 9561 unigenes were annotated. Comparative analysis identified 1259 genes expressed differentially in mycelium and yeast phase, and were categorized into a number of important biological processes, such as synthesis and metabolism, transmembrane transport, biocatalysis, oxidation reduction, and cellular signal transduction. CONCLUSIONS: The findings suggested that temperature-dependent transition was tightly associated with stress adaptation, growth and development, signal regulation, adhesion, and colonization, which was predicted to be related with virulence and pathogenesis. Collection of these data should offer fine-scale insights into the mechanisms of dimorphism and pathogenesis of S. schenckii, and meanwhile facilitate the evolutionary and function studies of other dimorphic fungi. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10123-020-00136-y) contains supplementary material, which is available to authorized users. Springer International Publishing 2020-07-20 2021 /pmc/articles/PMC7873001/ /pubmed/32691258 http://dx.doi.org/10.1007/s10123-020-00136-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Original Article He, Dan Zhang, Xiaowei Gao, Song You, Hailong Zhao, Yanbing Wang, Li Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress |
title | Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress |
title_full | Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress |
title_fullStr | Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress |
title_full_unstemmed | Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress |
title_short | Transcriptome Analysis of Dimorphic Fungus Sporothrix schenckii Exposed to Temperature Stress |
title_sort | transcriptome analysis of dimorphic fungus sporothrix schenckii exposed to temperature stress |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873001/ https://www.ncbi.nlm.nih.gov/pubmed/32691258 http://dx.doi.org/10.1007/s10123-020-00136-y |
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