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Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem

We clarified the genetic diversity of Trypanosoma spp. within the Kafue ecosystem, using PCR targeting the internal transcribed spacer 1 and the cathepsin L-like cysteine protease (CatL) sequences. The overall prevalence of Trypanosoma spp. in cattle and tsetse flies was 12.65 and 26.85%, respective...

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Autores principales: Nakamura, Yukiko, Hayashida, Kyoko, Delesalle, Victoire, Qiu, Yongjin, Omori, Ryosuke, Simuunza, Martin, Sugimoto, Chihiro, Namangala, Boniface, Yamagishi, Junya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873289/
https://www.ncbi.nlm.nih.gov/pubmed/33585616
http://dx.doi.org/10.3389/fvets.2021.599815
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author Nakamura, Yukiko
Hayashida, Kyoko
Delesalle, Victoire
Qiu, Yongjin
Omori, Ryosuke
Simuunza, Martin
Sugimoto, Chihiro
Namangala, Boniface
Yamagishi, Junya
author_facet Nakamura, Yukiko
Hayashida, Kyoko
Delesalle, Victoire
Qiu, Yongjin
Omori, Ryosuke
Simuunza, Martin
Sugimoto, Chihiro
Namangala, Boniface
Yamagishi, Junya
author_sort Nakamura, Yukiko
collection PubMed
description We clarified the genetic diversity of Trypanosoma spp. within the Kafue ecosystem, using PCR targeting the internal transcribed spacer 1 and the cathepsin L-like cysteine protease (CatL) sequences. The overall prevalence of Trypanosoma spp. in cattle and tsetse flies was 12.65 and 26.85%, respectively. Cattle positive for Trypanosoma vivax had a significantly lower packed cell volume, suggesting that T. vivax is the dominant Trypanosoma spp. causing anemia in this area. Among the 12 operational taxonomic units (OTUs) of T. vivax CatL sequences detected, one was from a known T. vivax lineage, two OTUs were from known T. vivax-like lineages, and nine OTUs were considered novel T. vivax-like lineages. These findings support previous reports that indicated the extensive diversity of T. vivax-like lineages. The findings also indicate that combining CatL PCR with next generation sequencing is useful in assessing Trypanosoma spp. diversity, especially for T. vivax and T. vivax-like lineages. In addition, the 5.42% prevalence of Trypanosoma brucei rhodesiense found in cattle raises concern in the community and requires careful monitoring of human African trypanosomiasis.
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spelling pubmed-78732892021-02-11 Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem Nakamura, Yukiko Hayashida, Kyoko Delesalle, Victoire Qiu, Yongjin Omori, Ryosuke Simuunza, Martin Sugimoto, Chihiro Namangala, Boniface Yamagishi, Junya Front Vet Sci Veterinary Science We clarified the genetic diversity of Trypanosoma spp. within the Kafue ecosystem, using PCR targeting the internal transcribed spacer 1 and the cathepsin L-like cysteine protease (CatL) sequences. The overall prevalence of Trypanosoma spp. in cattle and tsetse flies was 12.65 and 26.85%, respectively. Cattle positive for Trypanosoma vivax had a significantly lower packed cell volume, suggesting that T. vivax is the dominant Trypanosoma spp. causing anemia in this area. Among the 12 operational taxonomic units (OTUs) of T. vivax CatL sequences detected, one was from a known T. vivax lineage, two OTUs were from known T. vivax-like lineages, and nine OTUs were considered novel T. vivax-like lineages. These findings support previous reports that indicated the extensive diversity of T. vivax-like lineages. The findings also indicate that combining CatL PCR with next generation sequencing is useful in assessing Trypanosoma spp. diversity, especially for T. vivax and T. vivax-like lineages. In addition, the 5.42% prevalence of Trypanosoma brucei rhodesiense found in cattle raises concern in the community and requires careful monitoring of human African trypanosomiasis. Frontiers Media S.A. 2021-01-27 /pmc/articles/PMC7873289/ /pubmed/33585616 http://dx.doi.org/10.3389/fvets.2021.599815 Text en Copyright © 2021 Nakamura, Hayashida, Delesalle, Qiu, Omori, Simuunza, Sugimoto, Namangala and Yamagishi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Nakamura, Yukiko
Hayashida, Kyoko
Delesalle, Victoire
Qiu, Yongjin
Omori, Ryosuke
Simuunza, Martin
Sugimoto, Chihiro
Namangala, Boniface
Yamagishi, Junya
Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
title Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
title_full Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
title_fullStr Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
title_full_unstemmed Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
title_short Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
title_sort genetic diversity of african trypanosomes in tsetse flies and cattle from the kafue ecosystem
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873289/
https://www.ncbi.nlm.nih.gov/pubmed/33585616
http://dx.doi.org/10.3389/fvets.2021.599815
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