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Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem
We clarified the genetic diversity of Trypanosoma spp. within the Kafue ecosystem, using PCR targeting the internal transcribed spacer 1 and the cathepsin L-like cysteine protease (CatL) sequences. The overall prevalence of Trypanosoma spp. in cattle and tsetse flies was 12.65 and 26.85%, respective...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873289/ https://www.ncbi.nlm.nih.gov/pubmed/33585616 http://dx.doi.org/10.3389/fvets.2021.599815 |
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author | Nakamura, Yukiko Hayashida, Kyoko Delesalle, Victoire Qiu, Yongjin Omori, Ryosuke Simuunza, Martin Sugimoto, Chihiro Namangala, Boniface Yamagishi, Junya |
author_facet | Nakamura, Yukiko Hayashida, Kyoko Delesalle, Victoire Qiu, Yongjin Omori, Ryosuke Simuunza, Martin Sugimoto, Chihiro Namangala, Boniface Yamagishi, Junya |
author_sort | Nakamura, Yukiko |
collection | PubMed |
description | We clarified the genetic diversity of Trypanosoma spp. within the Kafue ecosystem, using PCR targeting the internal transcribed spacer 1 and the cathepsin L-like cysteine protease (CatL) sequences. The overall prevalence of Trypanosoma spp. in cattle and tsetse flies was 12.65 and 26.85%, respectively. Cattle positive for Trypanosoma vivax had a significantly lower packed cell volume, suggesting that T. vivax is the dominant Trypanosoma spp. causing anemia in this area. Among the 12 operational taxonomic units (OTUs) of T. vivax CatL sequences detected, one was from a known T. vivax lineage, two OTUs were from known T. vivax-like lineages, and nine OTUs were considered novel T. vivax-like lineages. These findings support previous reports that indicated the extensive diversity of T. vivax-like lineages. The findings also indicate that combining CatL PCR with next generation sequencing is useful in assessing Trypanosoma spp. diversity, especially for T. vivax and T. vivax-like lineages. In addition, the 5.42% prevalence of Trypanosoma brucei rhodesiense found in cattle raises concern in the community and requires careful monitoring of human African trypanosomiasis. |
format | Online Article Text |
id | pubmed-7873289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78732892021-02-11 Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem Nakamura, Yukiko Hayashida, Kyoko Delesalle, Victoire Qiu, Yongjin Omori, Ryosuke Simuunza, Martin Sugimoto, Chihiro Namangala, Boniface Yamagishi, Junya Front Vet Sci Veterinary Science We clarified the genetic diversity of Trypanosoma spp. within the Kafue ecosystem, using PCR targeting the internal transcribed spacer 1 and the cathepsin L-like cysteine protease (CatL) sequences. The overall prevalence of Trypanosoma spp. in cattle and tsetse flies was 12.65 and 26.85%, respectively. Cattle positive for Trypanosoma vivax had a significantly lower packed cell volume, suggesting that T. vivax is the dominant Trypanosoma spp. causing anemia in this area. Among the 12 operational taxonomic units (OTUs) of T. vivax CatL sequences detected, one was from a known T. vivax lineage, two OTUs were from known T. vivax-like lineages, and nine OTUs were considered novel T. vivax-like lineages. These findings support previous reports that indicated the extensive diversity of T. vivax-like lineages. The findings also indicate that combining CatL PCR with next generation sequencing is useful in assessing Trypanosoma spp. diversity, especially for T. vivax and T. vivax-like lineages. In addition, the 5.42% prevalence of Trypanosoma brucei rhodesiense found in cattle raises concern in the community and requires careful monitoring of human African trypanosomiasis. Frontiers Media S.A. 2021-01-27 /pmc/articles/PMC7873289/ /pubmed/33585616 http://dx.doi.org/10.3389/fvets.2021.599815 Text en Copyright © 2021 Nakamura, Hayashida, Delesalle, Qiu, Omori, Simuunza, Sugimoto, Namangala and Yamagishi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Nakamura, Yukiko Hayashida, Kyoko Delesalle, Victoire Qiu, Yongjin Omori, Ryosuke Simuunza, Martin Sugimoto, Chihiro Namangala, Boniface Yamagishi, Junya Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem |
title | Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem |
title_full | Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem |
title_fullStr | Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem |
title_full_unstemmed | Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem |
title_short | Genetic Diversity of African Trypanosomes in Tsetse Flies and Cattle From the Kafue Ecosystem |
title_sort | genetic diversity of african trypanosomes in tsetse flies and cattle from the kafue ecosystem |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873289/ https://www.ncbi.nlm.nih.gov/pubmed/33585616 http://dx.doi.org/10.3389/fvets.2021.599815 |
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