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Humoral and Cellular Response of Frontline Health Care Workers Infected by SARS-CoV-2 in Nice, France: A Prospective Single-Center Cohort Study

Frontline health care workers (HCWs) have been particularly exposed to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) since the start of the pandemic but the clinical features and immune responses of those infected with SARS-CoV-2 have not been well described. In a prospective single c...

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Autores principales: Cremoni, Marion, Ruetsch, Caroline, Zorzi, Kévin, Fernandez, Céline, Boyer-Suavet, Sonia, Benzaken, Sylvia, Demonchy, Elisa, Dellamonica, Jean, Ichai, Carole, Esnault, Vincent, Brglez, Vesna, Seitz-Polski, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873459/
https://www.ncbi.nlm.nih.gov/pubmed/33585509
http://dx.doi.org/10.3389/fmed.2020.608804
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author Cremoni, Marion
Ruetsch, Caroline
Zorzi, Kévin
Fernandez, Céline
Boyer-Suavet, Sonia
Benzaken, Sylvia
Demonchy, Elisa
Dellamonica, Jean
Ichai, Carole
Esnault, Vincent
Brglez, Vesna
Seitz-Polski, Barbara
author_facet Cremoni, Marion
Ruetsch, Caroline
Zorzi, Kévin
Fernandez, Céline
Boyer-Suavet, Sonia
Benzaken, Sylvia
Demonchy, Elisa
Dellamonica, Jean
Ichai, Carole
Esnault, Vincent
Brglez, Vesna
Seitz-Polski, Barbara
author_sort Cremoni, Marion
collection PubMed
description Frontline health care workers (HCWs) have been particularly exposed to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) since the start of the pandemic but the clinical features and immune responses of those infected with SARS-CoV-2 have not been well described. In a prospective single center cohort study, we enrolled 196 frontline HCWs exposed to the SARS-Cov-2 and 60 patients with moderate and severe forms of the coronavirus disease 2019 (COVID-19). Serological tests and cytokines assay were performed to analyze SARS-CoV-2-specific humoral and cellular immunity. Of the 196 HCWs tested, 15% had specific antibodies against SARS-CoV-2 and 45% of seropositive HCWs were strictly asymptomatic. However, in comparison to moderate and severe forms, HCWs with mild or asymptomatic forms of COVID-19 showed lower specific IgA and IgG peaks, consistent with their mild symptoms, and a robust immune cellular response, illustrated by a high production of type I and II interferons. Further studies are needed to evaluate whether this interferon functional immune assay, routinely applicable, can be useful in predicting the risk of severe forms of COVID-19.
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spelling pubmed-78734592021-02-11 Humoral and Cellular Response of Frontline Health Care Workers Infected by SARS-CoV-2 in Nice, France: A Prospective Single-Center Cohort Study Cremoni, Marion Ruetsch, Caroline Zorzi, Kévin Fernandez, Céline Boyer-Suavet, Sonia Benzaken, Sylvia Demonchy, Elisa Dellamonica, Jean Ichai, Carole Esnault, Vincent Brglez, Vesna Seitz-Polski, Barbara Front Med (Lausanne) Medicine Frontline health care workers (HCWs) have been particularly exposed to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) since the start of the pandemic but the clinical features and immune responses of those infected with SARS-CoV-2 have not been well described. In a prospective single center cohort study, we enrolled 196 frontline HCWs exposed to the SARS-Cov-2 and 60 patients with moderate and severe forms of the coronavirus disease 2019 (COVID-19). Serological tests and cytokines assay were performed to analyze SARS-CoV-2-specific humoral and cellular immunity. Of the 196 HCWs tested, 15% had specific antibodies against SARS-CoV-2 and 45% of seropositive HCWs were strictly asymptomatic. However, in comparison to moderate and severe forms, HCWs with mild or asymptomatic forms of COVID-19 showed lower specific IgA and IgG peaks, consistent with their mild symptoms, and a robust immune cellular response, illustrated by a high production of type I and II interferons. Further studies are needed to evaluate whether this interferon functional immune assay, routinely applicable, can be useful in predicting the risk of severe forms of COVID-19. Frontiers Media S.A. 2021-01-27 /pmc/articles/PMC7873459/ /pubmed/33585509 http://dx.doi.org/10.3389/fmed.2020.608804 Text en Copyright © 2021 Cremoni, Ruetsch, Zorzi, Fernandez, Boyer-Suavet, Benzaken, Demonchy, Dellamonica, Ichai, Esnault, Brglez and Seitz-Polski. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Cremoni, Marion
Ruetsch, Caroline
Zorzi, Kévin
Fernandez, Céline
Boyer-Suavet, Sonia
Benzaken, Sylvia
Demonchy, Elisa
Dellamonica, Jean
Ichai, Carole
Esnault, Vincent
Brglez, Vesna
Seitz-Polski, Barbara
Humoral and Cellular Response of Frontline Health Care Workers Infected by SARS-CoV-2 in Nice, France: A Prospective Single-Center Cohort Study
title Humoral and Cellular Response of Frontline Health Care Workers Infected by SARS-CoV-2 in Nice, France: A Prospective Single-Center Cohort Study
title_full Humoral and Cellular Response of Frontline Health Care Workers Infected by SARS-CoV-2 in Nice, France: A Prospective Single-Center Cohort Study
title_fullStr Humoral and Cellular Response of Frontline Health Care Workers Infected by SARS-CoV-2 in Nice, France: A Prospective Single-Center Cohort Study
title_full_unstemmed Humoral and Cellular Response of Frontline Health Care Workers Infected by SARS-CoV-2 in Nice, France: A Prospective Single-Center Cohort Study
title_short Humoral and Cellular Response of Frontline Health Care Workers Infected by SARS-CoV-2 in Nice, France: A Prospective Single-Center Cohort Study
title_sort humoral and cellular response of frontline health care workers infected by sars-cov-2 in nice, france: a prospective single-center cohort study
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873459/
https://www.ncbi.nlm.nih.gov/pubmed/33585509
http://dx.doi.org/10.3389/fmed.2020.608804
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