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Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species
BACKGROUND: Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial vertebr...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873661/ https://www.ncbi.nlm.nih.gov/pubmed/33568213 http://dx.doi.org/10.1186/s13071-021-04592-x |
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author | Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Fit, Nicodim Mihalca, Andrei Daniel |
author_facet | Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Fit, Nicodim Mihalca, Andrei Daniel |
author_sort | Matei, Ioana A. |
collection | PubMed |
description | BACKGROUND: Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial vertebrate groups. While the reservoir competence of many different vertebrate species is well known (e.g. birds, rodents and dogs), studies on insectivorous bats have been rarely performed despite their high species diversity, ubiquitous urban presence and importance in harboring zoonotic disease agents. Romania has a high diversity and ubiquity of bats. Moreover, seven out of eight SFG rickettsiae species with zoonotic potential were previously reported in Romania. Based on this, the aim of this study was to detect Rickettsia species in tissue samples in bats. METHODS: Here we report a large-scale study (322 bats belonging to 20 species) on the presence of Rickettsia spp. in Romanian bat species. Tissue samples from insectivorous bats were tested for the presence of Rickettsia DNA using PCR detection amplifying a 381 bp fragment of the gltA gene. Positive results were sequenced to confirm the results. The obtained results were statistically analyzed by chi-squared independence test. RESULTS: Positive results were obtained in 14.6% of bat samples. Sequence analysis confirmed the presence of R. monacensis in two bat species (Nyctalus noctula and Pipistrellus pipistrellus) in two locations. CONCLUSION: This study provides the first evidence of a possible involvement of these bat species in the epidemiology of Rickettsia spp., highlighting the importance of bats in natural cycles of these vector-borne pathogens. [Image: see text] |
format | Online Article Text |
id | pubmed-7873661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-78736612021-02-10 Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Fit, Nicodim Mihalca, Andrei Daniel Parasit Vectors Research BACKGROUND: Spotted fever group rickettsiae represent one of the most diverse groups of vector-borne bacteria, with several human pathogenic species showing an emerging trend worldwide. Most species are vectored by ticks (Ixodidae), with many zoonotic reservoir species among most terrestrial vertebrate groups. While the reservoir competence of many different vertebrate species is well known (e.g. birds, rodents and dogs), studies on insectivorous bats have been rarely performed despite their high species diversity, ubiquitous urban presence and importance in harboring zoonotic disease agents. Romania has a high diversity and ubiquity of bats. Moreover, seven out of eight SFG rickettsiae species with zoonotic potential were previously reported in Romania. Based on this, the aim of this study was to detect Rickettsia species in tissue samples in bats. METHODS: Here we report a large-scale study (322 bats belonging to 20 species) on the presence of Rickettsia spp. in Romanian bat species. Tissue samples from insectivorous bats were tested for the presence of Rickettsia DNA using PCR detection amplifying a 381 bp fragment of the gltA gene. Positive results were sequenced to confirm the results. The obtained results were statistically analyzed by chi-squared independence test. RESULTS: Positive results were obtained in 14.6% of bat samples. Sequence analysis confirmed the presence of R. monacensis in two bat species (Nyctalus noctula and Pipistrellus pipistrellus) in two locations. CONCLUSION: This study provides the first evidence of a possible involvement of these bat species in the epidemiology of Rickettsia spp., highlighting the importance of bats in natural cycles of these vector-borne pathogens. [Image: see text] BioMed Central 2021-02-10 /pmc/articles/PMC7873661/ /pubmed/33568213 http://dx.doi.org/10.1186/s13071-021-04592-x Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Matei, Ioana A. Corduneanu, Alexandra Sándor, Attila D. Ionică, Angela Monica Panait, Luciana Kalmár, Zsuzsa Ivan, Talida Papuc, Ionel Bouari, Cosmina Fit, Nicodim Mihalca, Andrei Daniel Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title | Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_full | Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_fullStr | Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_full_unstemmed | Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_short | Rickettsia spp. in bats of Romania: high prevalence of Rickettsia monacensis in two insectivorous bat species |
title_sort | rickettsia spp. in bats of romania: high prevalence of rickettsia monacensis in two insectivorous bat species |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873661/ https://www.ncbi.nlm.nih.gov/pubmed/33568213 http://dx.doi.org/10.1186/s13071-021-04592-x |
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