Biochemical detection of capsid in the nucleus during HIV-1 infection

To understand the role of the HIV-1 capsid in viral replication, we developed a protocol to biochemically track capsid in the nucleus during infection. To this end, we separated HIV-1-infected cells into nuclear and cytosolic fractions. Fractions were analyzed by western blotting for HIV-1 capsid co...

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Detalles Bibliográficos
Autores principales: Selyutina, Anastasia, Diaz-Griffero, Felipe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873705/
https://www.ncbi.nlm.nih.gov/pubmed/33604582
http://dx.doi.org/10.1016/j.xpro.2021.100323
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author Selyutina, Anastasia
Diaz-Griffero, Felipe
author_facet Selyutina, Anastasia
Diaz-Griffero, Felipe
author_sort Selyutina, Anastasia
collection PubMed
description To understand the role of the HIV-1 capsid in viral replication, we developed a protocol to biochemically track capsid in the nucleus during infection. To this end, we separated HIV-1-infected cells into nuclear and cytosolic fractions. Fractions were analyzed by western blotting for HIV-1 capsid content as well as for nuclear and cytosolic markers to assess the bona fide origin of the fractions. This protocol can be applied in both cycling and non-cycling human cells. For complete details on the use and execution of this protocol, please refer to Selyutina et al. (2020a).
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spelling pubmed-78737052021-02-17 Biochemical detection of capsid in the nucleus during HIV-1 infection Selyutina, Anastasia Diaz-Griffero, Felipe STAR Protoc Protocol To understand the role of the HIV-1 capsid in viral replication, we developed a protocol to biochemically track capsid in the nucleus during infection. To this end, we separated HIV-1-infected cells into nuclear and cytosolic fractions. Fractions were analyzed by western blotting for HIV-1 capsid content as well as for nuclear and cytosolic markers to assess the bona fide origin of the fractions. This protocol can be applied in both cycling and non-cycling human cells. For complete details on the use and execution of this protocol, please refer to Selyutina et al. (2020a). Elsevier 2021-02-03 /pmc/articles/PMC7873705/ /pubmed/33604582 http://dx.doi.org/10.1016/j.xpro.2021.100323 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Selyutina, Anastasia
Diaz-Griffero, Felipe
Biochemical detection of capsid in the nucleus during HIV-1 infection
title Biochemical detection of capsid in the nucleus during HIV-1 infection
title_full Biochemical detection of capsid in the nucleus during HIV-1 infection
title_fullStr Biochemical detection of capsid in the nucleus during HIV-1 infection
title_full_unstemmed Biochemical detection of capsid in the nucleus during HIV-1 infection
title_short Biochemical detection of capsid in the nucleus during HIV-1 infection
title_sort biochemical detection of capsid in the nucleus during hiv-1 infection
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873705/
https://www.ncbi.nlm.nih.gov/pubmed/33604582
http://dx.doi.org/10.1016/j.xpro.2021.100323
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