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Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice
Given the aggressive spread of COVID-19-related deaths, there is an urgent public health need to support the development of vaccine candidates to rapidly improve the available control measures against SARS-CoV-2. To meet this need, we are leveraging our existing vaccine platform to target SARS-CoV-2...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873992/ https://www.ncbi.nlm.nih.gov/pubmed/33584668 http://dx.doi.org/10.3389/fimmu.2020.602254 |
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author | Fisher, Eva Padula, Laura Podack, Kristin O’Neill, Katelyn Seavey, Matthew M. Jayaraman, Padmini Jasuja, Rahul Strbo, Natasa |
author_facet | Fisher, Eva Padula, Laura Podack, Kristin O’Neill, Katelyn Seavey, Matthew M. Jayaraman, Padmini Jasuja, Rahul Strbo, Natasa |
author_sort | Fisher, Eva |
collection | PubMed |
description | Given the aggressive spread of COVID-19-related deaths, there is an urgent public health need to support the development of vaccine candidates to rapidly improve the available control measures against SARS-CoV-2. To meet this need, we are leveraging our existing vaccine platform to target SARS-CoV-2. Here, we generated cellular heat shock chaperone protein, glycoprotein 96 (gp96), to deliver SARS-CoV-2 protein S (spike) to the immune system and to induce cell-mediated immune responses. We showed that our vaccine platform effectively stimulates a robust cellular immune response against protein S. Moreover, we confirmed that gp96-Ig, secreted from allogeneic cells expressing full-length protein S, generates powerful, protein S polyepitope-specific CD4+ and CD8+ T cell responses in both lung interstitium and airways. These findings were further strengthened by the observation that protein-S -specific CD8+ T cells were induced in human leukocyte antigen HLA-A2.1 transgenic mice thus providing encouraging translational data that the vaccine is likely to work in humans, in the context of SARS-CoV-2 antigen presentation. |
format | Online Article Text |
id | pubmed-7873992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78739922021-02-11 Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice Fisher, Eva Padula, Laura Podack, Kristin O’Neill, Katelyn Seavey, Matthew M. Jayaraman, Padmini Jasuja, Rahul Strbo, Natasa Front Immunol Immunology Given the aggressive spread of COVID-19-related deaths, there is an urgent public health need to support the development of vaccine candidates to rapidly improve the available control measures against SARS-CoV-2. To meet this need, we are leveraging our existing vaccine platform to target SARS-CoV-2. Here, we generated cellular heat shock chaperone protein, glycoprotein 96 (gp96), to deliver SARS-CoV-2 protein S (spike) to the immune system and to induce cell-mediated immune responses. We showed that our vaccine platform effectively stimulates a robust cellular immune response against protein S. Moreover, we confirmed that gp96-Ig, secreted from allogeneic cells expressing full-length protein S, generates powerful, protein S polyepitope-specific CD4+ and CD8+ T cell responses in both lung interstitium and airways. These findings were further strengthened by the observation that protein-S -specific CD8+ T cells were induced in human leukocyte antigen HLA-A2.1 transgenic mice thus providing encouraging translational data that the vaccine is likely to work in humans, in the context of SARS-CoV-2 antigen presentation. Frontiers Media S.A. 2021-01-26 /pmc/articles/PMC7873992/ /pubmed/33584668 http://dx.doi.org/10.3389/fimmu.2020.602254 Text en Copyright © 2021 Fisher, Padula, Podack, O’Neill, Seavey, Jayaraman, Jasuja and Strbo http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Fisher, Eva Padula, Laura Podack, Kristin O’Neill, Katelyn Seavey, Matthew M. Jayaraman, Padmini Jasuja, Rahul Strbo, Natasa Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice |
title | Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice |
title_full | Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice |
title_fullStr | Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice |
title_full_unstemmed | Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice |
title_short | Induction of SARS-CoV-2 Protein S-Specific CD8+ T Cells in the Lungs of gp96-Ig-S Vaccinated Mice |
title_sort | induction of sars-cov-2 protein s-specific cd8+ t cells in the lungs of gp96-ig-s vaccinated mice |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873992/ https://www.ncbi.nlm.nih.gov/pubmed/33584668 http://dx.doi.org/10.3389/fimmu.2020.602254 |
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